LOCUS       sequence-44728-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Glover JR, Lindquist S
  TITLE     Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues 
            previously aggregated proteins.
  JOURNAL   Cell 1998 Jul 10;94(1):73-82.
  PUBMED    9674429
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1998-07-10"; volume: "94(1)"; pages: "73-82"
COMMENT     Sequence Label: 6377 H10-104b
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..82
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             95..106
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggaattccct ctgaatattt tgtttacttt aagaaggaga tataccatgg gccatcatca
       61 tcatcatcat catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgaacgacca
      121 aacgcaattt acagaaaggg ctctaacgat tttgacgttg gctcaaaaat tggcttcgga
      181 tcatcaacat ccacaattac aacctataca tattctagct gccttcattg aaacgccaga
      241 agatggatca gtcccttacc tacagaatct aattgagaag ggccgttacg actatgatct
      301 tttcaagaaa gtggttaata gaaatctagt aagaattcct caacagcaac ctgcacctgc
      361 ggagataact ccaagttatg ctttggggaa agtccttcaa gacgctgcta agattcaaaa
      421 acaacagaag gactcattta tagcgcaaga ccatatattg tttgctctat tcaatgattc
      481 gtctattcag caaatattta aggaagctca agtagatatt gaggccatca agcaacaagc
      541 tcttgaactt cgtggtaaca ctagaattga ctctcgtggc gctgatacga acacaccttt
      601 ggaatattta tcaaagtacg ccattgatat gactgagcag gctcgtcaag gtaaacttga
      661 ccctgtcatc ggccgtgaag aagaaataag aagcactatt agagttttag caagaagaat
      721 taagtccaac ccatgtttaa ttggtgagcc aggtatcggt aagaccgcta ttattgaagg
      781 tgttgctcaa agaatcattg acgatgacgt tcccactatc ttacaaggcg ctaaattgtt
      841 cagtctagat ttggccgcat taaccgcagg tgctaaatac aaaggtgatt ttcgaagaaa
      901 gattcaaagg tgttttgaag gaaatcgaag aatcaaagac tcctaattgt gttattcatt
      961 ggatg
//
