LOCUS       sequence-447284-       11687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G16830.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G16830-mKOk
FEATURES             Location/Qualifiers
     source          1..11687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1534..1558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2539..2557)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2540..2557)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2586..2595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2625..2644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2626..2644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2651..2668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2651..2667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2660..2682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2825..3008
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3009..3050,3051..3059,3060..3830)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3090..3109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3700..3719
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4455..4707
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4800..4824
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5345..6136
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6382..6970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6871..6890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7124..7141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7156..7296
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7222..7241)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7382..7404
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7640..7834
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7900..8973)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9402..10031)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11362..11386
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11551..11572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11587..11592,11593..11610,11611..11617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11625..11641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11630..11652
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11649..11665
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11649..11665
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatgag tttccaagat ctcgaagctg
      721 gtactcgatc tccggcgccg aatcgtttta ccggtgggag acaacagaga ccttcgtctc
      781 gtggggatcc atctcaggag gtcgccgccg ggattttcag gatcagcacg gctgtcaatt
      841 ccttcttccg cctcgttaat tcgatcggaa cacctaaaga tactcttgaa ttacgagata
      901 agctgcaaaa gacaaggtta cagatatccg aattggtgaa gaatacttca gctaaactta
      961 aggaagctag cgaagccgac cttcatggat cagctagtca aattaagaag atagctgatg
     1021 ctaaactggc aaaagatttc caatcagttc tcaaagagtt tcagaaagct cagagacttg
     1081 cagctgaaag agaaataaca tatactcctg tggtcacaaa agaaatacct accagttata
     1141 atgcacctga actggatact gaatctttaa ggatctccca gcagcaagct cttcttctac
     1201 aatcaagaag gcaagaagtg gtgtttttag ataacgagat aacattcaat gaagcaatta
     1261 ttgaggaaag agagcaaggg attcgagaaa ttgaagacca gattcgtgat gtgaatggca
     1321 tgttcaaaga tctagctctt atggtgaacc atcaaggaaa tattgtcgat gacatcagct
     1381 ctaaccttga caactcacat gctgcaacta cacaagccac tgttcaactc agaaaagcag
     1441 ctaaaactca aagatcaaac tcctctttga catgcctact tattctcatt tttgggattg
     1501 ttctactcat tgtcatcatc gttgtcttgg tcgacccagc tttcttgtac aaagtggtga
     1561 tgggacgtcc gcggagatct acgcgtgtcg actcgagata tccaactagt atggtgagcg
     1621 ttatcaagcc tgagatgaag atgaggtact acatggacgg aagcgtgaac ggacacgagt
     1681 tcactattga aggcgaaggt actggaaggc cttacgaggg acatcaagag atgactctca
     1741 gagtgaccat ggctgaaggt ggacctatgc ctttcgcttt cgatctcgtg tctcacgtgt
     1801 tctgctacgg acacagagtg ttcactaagt accctgaaga gatcccggac tacttcaagc
     1861 aagctttccc tgagggactc tcttgggaga gatctttgga attcgaggac ggtggatccg
     1921 cttctgtgtc tgctcatatc tctctcaggg gcaacacctt ctaccacaag agcaagttta
     1981 ccggggtgaa cttccctgct gatggaccga tcatgcagaa ccagtctgtt gattgggagc
     2041 cgtctactga gaagatcacc gcttctgatg gtgtgctcaa gggtgatgtg accatgtacc
     2101 ttaagctcga aggtggtgga aaccacaagt gccagttcaa gactacctac aaggccgcca
     2161 aagaaatcct cgagatgcct ggtgatcact acatcggaca taggctcgtg agaaagaccg
     2221 agggaaacat cactgagcag gttgaggatg ctgtggctca ttctcaattg ttataagcgg
     2281 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     2341 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     2401 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     2461 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagctag
     2521 cttactagtg atgcatattc tatagtgtca cctaaatctg cggccgcact agtgatatcc
     2581 cgcggccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt
     2641 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     2701 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     2761 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     2821 taatgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
     2881 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
     2941 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caatccaaat
     3001 aatctgcaat ggcaattacc ttatccgcaa cttctttacc tatttccgcc cggatccggg
     3061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3181 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3541 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3841 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     3901 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     3961 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgatccaa cacttacgtt
     4021 tgcaacgtcc aagagcaaat agaccacgaa cgccggaagg ttgccgcagc gtgtggattg
     4081 cgtctcaatt ctctcttgca ggaatgcaat gatgaatatg atactgacta tgaaactttg
     4141 agggaatact gcctagcacc gtcacctcat aacgtgcatc atgcatgccc tgacaacatg
     4201 gaacatcgct atttttctga agaattatgc tcgttggagg atgtcgcggc aattgcagct
     4261 attgccaaaa tcgaaatacc cctcacgcat gcattcatca atattattca tgcggggaaa
     4321 ggcaagatta atccaactgg caaatcatcc agcgtgattg gtaacttcag ttccagcgac
     4381 ttgattcgtt ttggtgctac ccacgttttc aataaggacg agatggtgga gtaaagaagg
     4441 agtgcgtcga agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     4501 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     4561 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     4621 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     4681 gcgcggtgtc atctatgtta ctagatcgac cggcatgcaa gctgataatt caattcggcg
     4741 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     4801 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     4861 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     4921 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     4981 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     5041 agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt
     5101 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     5161 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     5221 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     5281 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     5341 catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     5401 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     5461 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     5521 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     5581 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     5641 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     5701 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     5761 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     5821 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     5881 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     5941 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     6001 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     6061 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     6121 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc
     6181 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     6241 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     6301 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7141 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7201 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     7261 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7321 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7381 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     7441 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     7501 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     7561 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     7621 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     7681 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     7741 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     7801 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     7861 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     7921 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     7981 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     8041 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     8101 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     8161 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     8221 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     8281 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     8341 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     8401 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     8461 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     8521 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     8581 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     8641 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     8701 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     8761 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     8821 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     8881 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     8941 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     9001 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
     9061 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     9121 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     9181 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     9241 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     9301 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     9361 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     9421 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     9481 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     9541 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     9601 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     9661 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     9721 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     9781 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     9841 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     9901 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     9961 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    10021 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    10081 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    10141 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    10201 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    10261 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    10321 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    10381 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    10441 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    10501 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    10561 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    10621 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    10681 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    10741 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    10801 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    10861 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    10921 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    10981 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    11041 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    11101 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    11161 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtacttta
    11221 aagtacttta aagtacttta aagtactttg atcccgaggg gaaccctgtg gttggcatgc
    11281 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    11341 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    11401 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc caatacgcaa
    11461 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11521 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11581 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    11641 atttcacaca ggaaacagct atgacatgat tacgaattcg agctcgg
//
