LOCUS       sequence-447287-       12125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Gateway DEST vector for expressing C-terminal tagged proteins 
            with mKusabiraOrangekand hygromycin selection in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(h)-DEST-mKOk
FEATURES             Location/Qualifiers
     source          1..12125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..793
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(818..823,824..841,842..848)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             902..1561
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(968..987)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1903..2208
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2087..2106
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2249..2373)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3455..3634
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3670..4695
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4893..5145
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5238..5262
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5783..6574
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6820..7408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7309..7328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7562..7579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7594..7734
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7660..7679)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7820..7842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8078..8272
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8338..9411)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9840..10469)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11800..11824
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11989..12010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12025..12030,12031..12048,12049..12055)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12063..12079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12068..12090
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12087..12103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12087..12103
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      721 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      781 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      841 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      901 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      961 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1021 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1081 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1141 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1201 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1261 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1321 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1381 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1441 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1501 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1561 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1621 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     1681 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1741 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1801 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1861 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     1921 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1981 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     2041 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     2101 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2161 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2221 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2281 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2341 cgtttctcgt tcagctttct tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg
     2401 tgtcgactcg agatatccaa ctagtatggt gagcgttatc aagcctgaga tgaagatgag
     2461 gtactacatg gacggaagcg tgaacggaca cgagttcact attgaaggcg aaggtactgg
     2521 aaggccttac gagggacatc aagagatgac tctcagagtg accatggctg aaggtggacc
     2581 tatgcctttc gctttcgatc tcgtgtctca cgtgttctgc tacggacaca gagtgttcac
     2641 taagtaccct gaagagatcc cggactactt caagcaagct ttccctgagg gactctcttg
     2701 ggagagatct ttggaattcg aggacggtgg atccgcttct gtgtctgctc atatctctct
     2761 caggggcaac accttctacc acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg
     2821 accgatcatg cagaaccagt ctgttgattg ggagccgtct actgagaaga tcaccgcttc
     2881 tgatggtgtg ctcaagggtg atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca
     2941 caagtgccag ttcaagacta cctacaaggc cgccaaagaa atcctcgaga tgcctggtga
     3001 tcactacatc ggacataggc tcgtgagaaa gaccgaggga aacatcactg agcaggttga
     3061 ggatgctgtg gctcattctc aattgttata agcggccatg ctagagtccg caaaaatcac
     3121 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     3181 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     3241 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     3301 accaaaatcc agtgacctgc aggcatgcga cgtcgggccc tctagaggat ccccgggtac
     3361 cgcgaattat cgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga
     3421 cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa ggagccactg
     3481 agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca ttattgcgcg
     3541 ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa aatgctccac
     3601 tgacgttcca taaattcccc tcggtatcca attagagtct catattcact ctcaactcga
     3661 atccccccta tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     3721 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3781 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3841 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3901 cttgacattg gggagttcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3961 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttcttcagcc ggtcgcggag
     4021 gctatggatg ctatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4081 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4141 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     4201 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     4261 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     4321 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     4381 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     4441 ggatcgccaa ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     4501 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     4561 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     4621 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     4681 agggcaaagg aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc
     4741 cagcaactcg cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca
     4801 cagttctcgt ccacagttcg ctaagctcgc tcggctgggt cgcgggaggg ccggtcgcag
     4861 tgattcagga attaattccc tagagtcaag cagatcgttc aaacatttgg caataaagtt
     4921 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     4981 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     5041 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     5101 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaccg gcatgcaagc
     5161 tgataattca attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     5221 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     5281 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     5341 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     5401 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     5461 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     5521 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     5581 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     5641 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     5701 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     5761 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     5821 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     5881 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     5941 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     6001 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     6061 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     6121 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     6181 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     6241 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     6301 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     6361 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     6421 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     6481 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     6541 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     6601 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     6661 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     6721 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     6781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6961 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7441 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7501 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7561 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     7621 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     7681 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     7741 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7801 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7861 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     7921 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     7981 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     8041 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     8101 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     8161 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     8221 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     8281 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     8341 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     8401 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     8461 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     8521 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     8581 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     8641 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     8701 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     8761 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     8821 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     8881 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     8941 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     9001 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     9061 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     9121 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     9181 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     9241 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     9301 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     9361 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     9421 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
     9481 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
     9541 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
     9601 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
     9661 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
     9721 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
     9781 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
     9841 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
     9901 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
     9961 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    10021 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    10081 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    10141 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    10201 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    10261 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    10321 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    10381 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    10441 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    10501 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    10561 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    10621 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    10681 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    10741 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    10801 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    10861 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    10921 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    10981 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    11041 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    11101 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    11161 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    11221 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    11281 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    11341 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    11401 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    11461 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    11521 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    11581 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    11641 ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat cccgagggga
    11701 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
    11761 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
    11821 gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct
    11881 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    11941 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    12001 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    12061 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag
    12121 ctcgg
//
