LOCUS       sequence-447291-       14321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS of CPH from N. nambi codon optimized for N.benthamiana. This 
            gene is the final step and recycling of the bioluminiscente pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 14321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB3774
FEATURES             Location/Qualifiers
     source          1..14321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             364..750
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             757..777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             781..801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             805..825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             826..975
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             976..996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1000..1020
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1021..1377
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1402..1971
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      1998..2250
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             2839..2859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2860..2883,2884..2886,2887..5370,5371..5376,5377..5442,
                     5443..5445,5446..6960)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2860..2883,2884..2886,2887..5373,5374..5376,
                     5377..6960)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2860..6960
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6979..6999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7003..7023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7027..7047
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7146..7398
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7636..7660
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8961..9590
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10019..11092
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11158..11352
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11588..11610)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11696..11836
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11751..11770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11851..11868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12022..12610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12102..12121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12698..13489)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    14275..14299
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaatg
      361 atggcttcta attttactca atttgttctt gttgataatg gtggtactgg tgatgttact
      421 gttgctcctt ctaattttgc taatggtgtt gctgaatgga tttcttctaa ttctagatct
      481 caagcttata aggttacttg ttctgttaga caatcttctg ctcaaaatag aaagtatact
      541 attaaggttg aagttcctaa ggttgctact caaactgttg gtggtgttga acttcctgtt
      601 gctgcttgga gatcttatct taatatggaa cttactattc ctatttttgc tactaattct
      661 gattgtgaac ttattgttaa ggctatgcaa ggtcttctta aggatggtaa tcctattcct
      721 tctgctattg ctgctaattc tggtatttat tcggacccaa aaaagaagcg taaggtcgat
      781 cccaagaaga agagaaaggt agatcctaag aagaagagaa aggtagacgc attggatgac
      841 ttcgacttag acatgcttgg gtcagatgct ttggacgact ttgacctcga catgttgggt
      901 tctgacgcgc tagatgactt cgacttggac atgttgggct ccgacgcttt ggacgacttt
      961 gatctggaca tgttaccgaa aaagaaacgc aaagttgatc ctaagaagaa gagaaaggta
     1021 cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca gttcgacgac
     1081 gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac cgacctggcc
     1141 agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt ggcccctcac
     1201 accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt gacaggcgct
     1261 cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc taatggactg
     1321 ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc cttgctgatt
     1381 aactctagaa gttccggatc tcgggattcc agggaaggga tgtttttgcc gaagcctgag
     1441 gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc aaaacgaatc
     1501 cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc cagcctcgca
     1561 ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc accagtccct
     1621 cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt ggaggatccc
     1681 gatgaagaaa cgagccaggc tgtcaaagcc cttcgggaga tggccgatac tgtgattccc
     1741 cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc cccaaggggc
     1801 catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa cctggactca
     1861 cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga gtgcctcttg
     1921 catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt ttgagcttgg
     1981 aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     2041 ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     2101 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     2161 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     2221 cgcgcgcggt gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga
     2281 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg
     2461 gaccgactcg cgctgtcagg agagtggtct tgggagagcg atgcgtcttt tccgatgaac
     2521 cgttccagca aaaaagacta agcgatgcgt cttttccgct gaaccgttcc agcaaaaaag
     2581 actaagcgat gcgtcttttc cgctgaaccg ttccagcaaa aaagactagg agagcgatgc
     2641 gtcttttccg ctgaaccgtt ccagcaaaaa agactatccc gtcctaatac ctctacgtgc
     2701 aagcagtata aaaacaaaat acataaacat aactaaaatc tcatatcgtt gtaattgtca
     2761 tatactagtg gtaattaagt ccagttacac ttaaggttaa atttgctgac tcttttttac
     2821 ctttcatttc tgaaaatgcc caagaagaag aggaaggtgg acaagaagta ctccattggg
     2881 ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
     2941 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
     3001 ggcgccctcc tgttcgactc cggggaaacg gccgaagcca cgcggctcaa aagaacagca
     3061 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
     3121 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
     3181 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
     3241 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
     3301 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
     3361 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
     3421 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
     3481 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
     3541 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
     3601 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
     3661 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
     3721 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
     3781 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
     3841 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
     3901 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
     3961 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
     4021 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
     4081 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
     4141 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
     4201 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
     4261 tcagaagaga ctatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
     4321 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
     4381 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
     4441 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
     4501 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagattat
     4561 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
     4621 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
     4681 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
     4741 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
     4801 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
     4861 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
     4921 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
     4981 cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca
     5041 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
     5101 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
     5161 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
     5221 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
     5281 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
     5341 gacatcaatc ggctctccga ctacgacgtg gatgccatcg tgccccagtc ttttctcaaa
     5401 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat
     5461 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
     5521 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
     5581 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
     5641 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
     5701 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtttcaga tttcagaaag
     5761 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
     5821 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
     5881 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
     5941 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
     6001 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
     6061 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
     6121 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
     6181 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
     6241 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
     6301 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
     6361 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
     6421 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
     6481 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
     6541 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaaggatct
     6601 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
     6661 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
     6721 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
     6781 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
     6841 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
     6901 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
     6961 agcagggctg attcggatcc taaaaagaag cgaaaggtag acccgaagaa aaagaggaag
     7021 gtagatccaa agaagaagcg caaggtagaa gtgtttgaat ttgaatatct ggatgataaa
     7081 gtgctggaag aactgctgga tagcgaagaa cgcaaacgct gagcttggaa tggatcttcg
     7141 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     7201 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     7261 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     7321 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     7381 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
     7441 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7501 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7561 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
     7621 cttgagaccg atatctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     7681 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     7741 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     7801 ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca
     7861 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     7921 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     7981 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     8041 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     8101 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     8161 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     8221 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     8281 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     8341 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     8401 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     8461 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     8521 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     8581 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     8641 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     8701 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     8761 ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     8821 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     8881 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     8941 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     9001 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     9061 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     9121 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     9181 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     9241 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     9301 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     9361 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     9421 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     9481 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     9541 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     9601 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     9661 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     9721 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     9781 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     9841 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     9901 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     9961 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
    10021 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
    10081 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
    10141 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
    10201 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
    10261 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
    10321 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
    10381 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
    10441 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
    10501 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
    10561 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
    10621 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
    10681 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
    10741 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
    10801 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
    10861 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
    10921 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
    10981 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
    11041 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
    11101 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt
    11161 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
    11221 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
    11281 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
    11341 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
    11401 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
    11461 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
    11521 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
    11581 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg
    11641 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
    11701 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
    11761 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11821 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    12181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    12241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    12301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    12361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    12421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    12481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12661 ctcacgttaa gggattttgg tcatgcattc taggtgatta tttgccgact accttggtga
    12721 tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat
    12781 cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg
    12841 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg
    12901 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcacca gcccagtcgg
    12961 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa
    13021 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt
    13081 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa
    13141 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa
    13201 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag
    13261 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc
    13321 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg
    13381 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa
    13441 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata atgtttaact
    13501 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
    13561 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa
    13621 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
    13681 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac
    13741 aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
    13801 ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggaacagaac ttattatttc
    13861 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    13921 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    13981 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    14041 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    14101 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    14161 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    14221 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    14281 gatatattgt ggtgtaaaca taacgatatc ggtctcagtc a
//
