LOCUS       sequence-447303-       10859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT1G22850.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(b)-AT1G22850-mKOk
FEATURES             Location/Qualifiers
     source          1..10859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1729..1753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2835..3014
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3056..3607
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3627..3879
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3972..3996
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4517..5308
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5554..6142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6043..6062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6296..6313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6328..6468
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6394..6413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6554..6576
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6812..7006
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7072..8145)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8574..9203)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10534..10558
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    10723..10744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10759..10764,10765..10782,10783..10789)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10797..10813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10802..10824
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10821..10837
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10821..10837
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gcgcagcctc actctaggtc
      721 tgactctcag atcctcctcc tcctcttctc ttccattcac ctgcaactcc cgcttctgct
      781 ctcctcctcc tccttcttcg tcctttagac tctacaaacg attccatttc cttaaaccat
      841 gctcttccct aaagcaaacc aagaagaaga agcaacagtc tctcccatct accgctccgc
      901 ctcctcagag tctccggtgg ttcttcaatt ctaaatccac taatgatgaa aacgacgaag
      961 atgatgttaa gtctgagagc gatgatgatg gcggatcgga aggtgatgct gctattaagg
     1021 gtactatctt agccggagtt ttgttgattg gtactgttgg tggattcgcc ggcgttggat
     1081 atgtctacag ggatcagatc aatactttcc ttactcaatt ctccacttac atcgaaggtt
     1141 atggtacggc tgggtatgcg ttattcattg ccgtctatgc tgggcttgag atactagcca
     1201 ttccagctct cccattgacg atgtcggctg gtcttctctt cggtccactc attggcacta
     1261 tcatagtttc tatcagtgga actatggctg ctagtgttgc tttcttaatt gctagatatt
     1321 ttgcccgaga gcgcatcctt aagttagttg aagacaacaa gaagttcctc gccattgata
     1381 aagcgattgg tgaaaatggg tttagagttg ttactctcct tcggctaagc cctctacttc
     1441 ctttctctct tggcaattac ctgtacgggt tgacgtctgt gaagtttgtg ccttatgtat
     1501 taggaagctg gttgggtatg cttccaggct cttgggctta tgtcagcgcc ggggcttttg
     1561 gacgagcaat cattcaagaa gaatctaatg ttggcttgcc tggaggaaac ggccagcttt
     1621 taactctcgg ggtgggtctg ttggttactg cattggctgg aacatatgtg acaagccttg
     1681 caaaggatgc tataaaggat attgatgatg atgagaagag agatgcacac ccagctttct
     1741 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     1801 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     1861 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     1921 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     1981 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     2041 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     2101 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     2161 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     2221 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     2281 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     2341 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     2401 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     2461 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2521 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2581 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2641 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     2701 aggcatgcga cgtcgggccc tctagaggat ccccgggtac cgcgaattat cgatcatgag
     2761 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
     2821 gtttggaact gacagaaccg caacgttgaa ggagccactg agccgcgggt ttctggagtt
     2881 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     2941 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     3001 tcggtatcca attagagtct catattcact ctcaactcga tcgaggggat ctaccatgag
     3061 cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg
     3121 caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga
     3181 accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc
     3241 cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt ccctggaagg cacgcaacgc
     3301 ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc ggacgggact
     3361 gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt
     3421 ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata
     3481 tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg
     3541 tttctggcag ctggacttca gcctgccggt gccgccccgt ccggtcctgc ccgtcaccga
     3601 aatctgatga cccctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     3661 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     3721 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     3781 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     3841 ataaattatc gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa
     3901 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     3961 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     4021 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     4081 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     4141 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     4201 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     4261 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     4321 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     4381 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     4441 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     4501 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     4561 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     4621 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     4681 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     4741 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     4801 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4861 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     4921 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     4981 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     5041 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     5101 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     5161 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     5221 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     5281 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     5341 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     5401 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     5461 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5701 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5821 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5881 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5941 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6001 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6061 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6121 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6181 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6241 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6301 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     6361 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6421 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6481 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6541 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6601 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     6661 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     6721 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     6781 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     6841 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     6901 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     6961 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     7021 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     7081 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     7141 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     7201 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     7261 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     7321 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     7381 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     7441 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     7501 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     7561 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     7621 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     7681 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     7741 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     7801 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     7861 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     7921 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     7981 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     8041 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     8101 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     8161 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     8221 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     8281 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     8341 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     8401 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     8461 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     8521 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     8581 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     8641 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     8701 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     8761 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     8821 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     8881 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     8941 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     9001 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     9061 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     9121 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     9181 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     9241 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     9301 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     9361 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     9421 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     9481 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     9541 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     9601 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     9661 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     9721 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     9781 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     9841 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     9901 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     9961 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    10021 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    10081 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    10141 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    10201 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    10261 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    10321 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    10381 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
    10441 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    10501 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
    10561 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta
    10621 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    10681 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10741 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10801 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcgg
//
