LOCUS       sequence-447306-        2332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the native promoter from the tetL tetracycline resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2332)
  TITLE     Cultivarium plasmids
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGLU_63
FEATURES             Location/Qualifiers
     source          1..2332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(376..392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(389..411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(564..1152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(644..663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1285..1379)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1400..2059)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1974..1993
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2060..2162)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2249..2271
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2263..2280
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2264..2280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgactcagg tctcacgtaa gtatctgcta cggctagccg ttccgcagcc acacgccgtg
       61 tgaagctggg gattttatga ccgatgatga agaaaagaat ctgaaactta gtttatatgt
      121 ggtaaaatgt tttaatcaag tttaggagga attaattatg aagtgtaatt aatgtaacag
      181 ggttcaatta aaagagggaa gcgtatcatt aaccctataa actacgtctg ccctcattat
      241 tggagggtga aataatgtga gaccgggatt cacattggat acgagatccg tagattgata
      301 agaggacacg gaatatccca ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
      361 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
      421 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
      481 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      541 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      601 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      661 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      721 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      781 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      841 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      901 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      961 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1021 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1081 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1141 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     1201 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg
     1261 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1321 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1381 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1441 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1501 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1561 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1621 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1681 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1741 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1801 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1861 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1921 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1981 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2041 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2101 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     2161 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     2221 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     2281 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac ct
//
