LOCUS       sequence-447308-       13277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT4G33650.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13277)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 13277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT4G33650-mKOk
FEATURES             Location/Qualifiers
     source          1..13277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3124..3148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4129..4147)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4130..4147)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      4176..4185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4215..4234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4216..4234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4241..4258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4241..4257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4250..4272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        4415..4598
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4599..4640,4641..4649,4650..5420)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4680..4699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5290..5309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6045..6297
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6390..6414
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6935..7726
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7972..8560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8461..8480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8714..8731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8746..8886
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8812..8831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8972..8994
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9230..9424
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9490..10563)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10992..11621)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12952..12976
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13141..13162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13177..13182,13183..13200,13201..13207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13215..13231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13220..13242
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13239..13255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13239..13255
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gactattgaa gaagtttccg
      721 gtgagactcc tccgtctacg cctccttctt cttctacgcc ttctccgtct tcctccacca
      781 ccaacgccgc gccgttggga tcctctgtga ttccgattgt aaacaagcta caagacatat
      841 tcgctcagct cggaagccag tctacgatcg cgcttcctca ggttgttgtt gttggaagcc
      901 aaagcagtgg caagtctagc gtccttgaag cactcgtcgg ccgtgacttt ctccctcgtg
      961 gtaatgatat ctgcacgcgt cgtcctcttg ttctccagct cctccagact aaaagccgcg
     1021 ctaatggcgg atccgatgat gagtgggggg agtttcgtca ccttcctgaa actcgtttct
     1081 atgatttctc tgagattcgt cgagaaattg aggctgagac gaatagatta gttggagaga
     1141 acaaaggtgt agcagataca cagattcgtc ttaaaatttc ttcacctaat gtattgaaca
     1201 tcacgcttgt ggatctgcct ggtattacca aggtgccagt tggtgaccag ccatccgaca
     1261 ttgaagcacg tataagaacg atgatcttgt cttacatcaa gcaagatact tgcttgatat
     1321 tggctgttac ccctgctaat accgatctag caaactctga tgcccttcaa attgctagca
     1381 ttgtagatcc tgatggtcac agaacgatag gtgtaatcac aaagttggat attatggaca
     1441 aaggtactga tgctcgaaaa ctccttcttg gaaatgttgt tcctcttcga cttggatacg
     1501 tgggagttgt aaatcgttgc caggaggata ttttgctaaa ccgcacagtc aaggaagcac
     1561 ttctcgcaga ggagaagttc ttccggagtc acccggttta ccatggtctt gccgatcgtt
     1621 tgggtgttcc tcagctagca aagaagttaa atcagatcct tgttcaacat atcaaggtct
     1681 tgcttccgga tttgaagtcg cggataagta atgctttggt tgctacagca aaagagcatc
     1741 agagctatgg tgaattaaca gaatcaaggg ctgggcaagg agctcttctc ctcaactttc
     1801 tctccaaata ctgtgaagca tactcttcat tgctggaagg gaaaagtgaa gaaatgtcta
     1861 cgtcagagct ctctggagga gcaagaattc actatatttt ccagtcaatc tttgttaaga
     1921 gtttggagga ggttgatcca tgcgaggact tgacagatga tgatattcgg actgcaattc
     1981 agaatgcaac tggtccaaga tctgcattat ttgttccaga tgttccattt gaagttcttg
     2041 ttaggaggca gatatctcgt ttgttagatc ctagccttca gtgtgcccgg ttcatttttg
     2101 aagagctaat aaagattagc catagatgta tgatgaatga gttacaacga tttccagtcc
     2161 tacgaaagcg catggatgag gttatcgggg atttcctgcg agaaggtctt gaaccctcag
     2221 aagcaatgat cggggatatc attgatatgg agatggatta cataaacact tcacatccaa
     2281 attttattgg tggaaccaag gccgtggaag ctgcaatgca tcaagtgaag tcttcgagga
     2341 ttccccatcc tgtggcacga ccaaaggaca ctgtggagcc tgatagaaca tcttcttcca
     2401 cgagtcaagt gaaatctaga tcgtttctcg gcaggcaagc taatggaatt gttactgatc
     2461 agggagttgt atctgcagat gctgaaaaag ctcaacctgc tgcaaatgcg agtgatacaa
     2521 ggtggggtat cccttcaatt ttccgagggg gtgatactag agcagttact aaagatagct
     2581 tattaaacaa accattcagt gaagctgttg aggatatgtc tcacaactta tccatgatct
     2641 atctaaagga gcccccagct gtcttgaggc caaccgaaac ccattcagaa caggaagcag
     2701 ttgagattca gataacaaag ctgttactaa gatcatacta tgacattgta aggaagaata
     2761 ttgaggactc ggtaccaaaa gcaatcatgc atttcctggt aaaccacaca aaacgtgagc
     2821 tgcataacgt cttcatcaag aagctttaca gggagaactt gtttgaagaa atgttgcaag
     2881 agccagatga gatagcagtt aagaggaaac gcacacaaga gactctccac gttcttcagc
     2941 aagcttacag gacactagat gagttgccac tggaagcaga ttcggtgtca gcaggaatgt
     3001 caaaacatca ggagttactg acatcttcaa agtattcgac aagcagctca tactcggcat
     3061 ctccaagtac gacaagaaga tcgagaagag caggagatca acaccaaaat ggatacggat
     3121 tccacccagc tttcttgtac aaagtggtga tgggacgtcc gcggagatct acgcgtgtcg
     3181 actcgagata tccaactagt atggtgagcg ttatcaagcc tgagatgaag atgaggtact
     3241 acatggacgg aagcgtgaac ggacacgagt tcactattga aggcgaaggt actggaaggc
     3301 cttacgaggg acatcaagag atgactctca gagtgaccat ggctgaaggt ggacctatgc
     3361 ctttcgcttt cgatctcgtg tctcacgtgt tctgctacgg acacagagtg ttcactaagt
     3421 accctgaaga gatcccggac tacttcaagc aagctttccc tgagggactc tcttgggaga
     3481 gatctttgga attcgaggac ggtggatccg cttctgtgtc tgctcatatc tctctcaggg
     3541 gcaacacctt ctaccacaag agcaagttta ccggggtgaa cttccctgct gatggaccga
     3601 tcatgcagaa ccagtctgtt gattgggagc cgtctactga gaagatcacc gcttctgatg
     3661 gtgtgctcaa gggtgatgtg accatgtacc ttaagctcga aggtggtgga aaccacaagt
     3721 gccagttcaa gactacctac aaggccgcca aagaaatcct cgagatgcct ggtgatcact
     3781 acatcggaca taggctcgtg agaaagaccg agggaaacat cactgagcag gttgaggatg
     3841 ctgtggctca ttctcaattg ttataagcgg ccatgctaga gtccgcaaaa atcaccagtc
     3901 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     3961 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     4021 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     4081 aatccagtga cctgcaggca tgcaagctag cttactagtg atgcatattc tatagtgtca
     4141 cctaaatctg cggccgcact agtgatatcc cgcggccatg gcggccggga gcatgcgacg
     4201 tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     4261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4381 gcctgaatgg cgaatggaaa ttgtaagcgt taatgggttt ctggagttta atgagctaag
     4441 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     4501 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     4561 tagagtctca tattcactct caatccaaat aatctgcaat ggcaattacc ttatccgcaa
     4621 cttctttacc tatttccgcc cggatccggg caggttctcc ggccgcttgg gtggagaggc
     4681 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4741 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4801 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4861 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4921 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4981 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5041 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5101 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5161 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5221 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5281 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5341 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5401 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5461 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5521 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5581 cttcgcccac cccgatccaa cacttacgtt tgcaacgtcc aagagcaaat agaccacgaa
     5641 cgccggaagg ttgccgcagc gtgtggattg cgtctcaatt ctctcttgca ggaatgcaat
     5701 gatgaatatg atactgacta tgaaactttg agggaatact gcctagcacc gtcacctcat
     5761 aacgtgcatc atgcatgccc tgacaacatg gaacatcgct atttttctga agaattatgc
     5821 tcgttggagg atgtcgcggc aattgcagct attgccaaaa tcgaaatacc cctcacgcat
     5881 gcattcatca atattattca tgcggggaaa ggcaagatta atccaactgg caaatcatcc
     5941 agcgtgattg gtaacttcag ttccagcgac ttgattcgtt ttggtgctac ccacgttttc
     6001 aataaggacg agatggtgga gtaaagaagg agtgcgtcga agcagatcgt tcaaacattt
     6061 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     6121 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     6181 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     6241 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac
     6301 cggcatgcaa gctgataatt caattcggcg ttaattcagt acattaaaaa cgtccgcaat
     6361 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     6421 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     6481 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     6541 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     6601 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc
     6661 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     6721 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     6781 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     6841 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     6901 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     6961 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     7021 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     7081 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     7141 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     7201 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     7261 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     7321 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     7381 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     7441 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     7501 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     7561 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     7621 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     7681 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     7741 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc
     7801 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     7861 ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga
     7921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8101 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     8761 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     8821 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     8881 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8941 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9001 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     9061 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     9121 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     9181 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     9241 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     9301 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     9361 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     9421 cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     9481 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     9541 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     9601 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     9661 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     9721 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     9781 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     9841 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     9901 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     9961 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
    10021 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
    10081 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
    10141 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
    10201 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
    10261 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
    10321 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
    10381 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
    10441 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
    10501 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
    10561 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
    10621 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
    10681 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
    10741 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
    10801 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
    10861 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
    10921 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
    10981 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
    11041 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
    11101 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
    11161 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
    11221 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
    11281 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
    11341 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
    11401 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
    11461 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
    11521 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
    11581 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
    11641 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
    11701 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
    11761 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
    11821 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
    11881 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
    11941 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
    12001 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
    12061 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
    12121 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
    12181 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
    12241 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
    12301 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
    12361 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
    12421 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
    12481 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
    12541 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    12601 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    12661 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    12721 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    12781 gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta aagtactttg
    12841 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    12901 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
    12961 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
    13021 caagctcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    13081 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    13141 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    13201 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    13261 tacgaattcg agctcgg
//
