LOCUS       sequence-447311-       12683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G40280.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G42080-mKOk
FEATURES             Location/Qualifiers
     source          1..12683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2530..2554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3535..3553)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3536..3553)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3582..3591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3621..3640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3622..3640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3647..3664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3647..3663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3656..3678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3821..4004
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4005..4046,4047..4055,4056..4826)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4086..4105)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4696..4715
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5451..5703
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5796..5820
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6341..7132
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7378..7966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7867..7886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8120..8137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8152..8292
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8218..8237)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8378..8400
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8636..8830
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8896..9969)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10398..11027)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12358..12382
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12547..12568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12583..12588,12589..12606,12607..12613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12621..12637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12626..12648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12645..12661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12645..12661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggaaaatctg atctctctgg
      721 ttaacaagat acagagagct tgcacggctt taggagacca tggagactcc agcgctttac
      781 ctactctttg ggattccttg cctgcgatcg ccgtcgttgg tggtcagagc tcagggaagt
      841 cttcagtcct ggagagcatc gtgggaaagg actttttacc ccgtggatct ggcattgtta
      901 ctcgaaggcc ccttgtctta cagttgcaaa agatcgatga tggaacccgg gagtatgcag
      961 agtttcttca cctcccgagg aaaaagttta ctgattttgc tgctgtgagg aaggagattc
     1021 aagatgagac tgacagagag actggacgca gcaaggctat ttctagtgtt cccattcacc
     1081 ttagcatata ctctcccaat gttgtcaact tgacactgat agatcttcca gggcttacaa
     1141 aagttgctgt tgatggacaa tctgatagta tagtgaagga cattgaaaac atggttcggt
     1201 cctacattga aaagcccaac tgcatcattt tggcaatctc acctgcaaac caagatcttg
     1261 ctacctcaga tgcaattaaa atttcccgtg aggttgatcc atcgggggac agaacatttg
     1321 gtgtcttgac aaagattgat cttatggaca aggggacgga tgcagtggaa attctggaag
     1381 ggagatcttt taaacttaaa tatccgtggg ttggtgtcgt caaccgttcc caagcagata
     1441 ttaacaagaa tgtcgacatg attgcggctc ggaaaagaga gagggagtac ttttccaata
     1501 ctactgagta taggcacctt gctaataaaa tgggttccga gcatttggca aagatgctct
     1561 ccaagcatct agaacgtgtg atcaagtcga gaattcctgg cattcagtca cttattaaca
     1621 aaacagtatt agagctggaa actgaactaa gtcgccttgg aaagcctatt gcagctgatg
     1681 caggggggaa gttgtactca ataatggaga tatgtcggct ttttgatcaa atattcaaag
     1741 agcatcttga tggagtgcgt gctggtggtg aaaaagtgta caacgtgttt gataaccagc
     1801 ttcctgcggc tctgaagaga ctccaatttg acaagcagct agcgatggac aacatccgga
     1861 agctggtcac tgaggctgat ggttaccagc ctcacttgat tgctcctgag caaggttacc
     1921 gtcgtctcat tgagtcttct attgtctcca tcagaggccc tgctgaagca tctgttgaca
     1981 ccgttcatgc tatcttaaag gatctggttc acaagtctgt gaatgaaact gtggaactaa
     2041 aacaataccc agctctgaga gtggaggtga caaatgcggc gatagagtcg ctggataaaa
     2101 tgcgggaagg aagtaagaaa gcaacactgc agctggttga catggagtgc agttacctca
     2161 ctgttgattt cttcaggaaa cttccccagg atgttgagaa gggtggtaac cccacacact
     2221 ccattttcga ccgctacaac gattcctatc tcagacgaat cggatccaat gttttgtctt
     2281 acgtgaacat ggtctgtgct ggcctgcgga attcaatccc caagtccatc gtatactgcc
     2341 aagtccgaga agcgaagcgc agtctcctcg accatttctt tgcggagctc ggtaccatgg
     2401 atatgaagag gctctcgtcg ctattgaacg aagatccagc aatcatggag agacgcagtg
     2461 ccatctcaaa gcggctagaa ttgtatcgag cagcccaatc cgagatcgat gctgttgctt
     2521 ggtccaagca cccagctttc ttgtacaaag tggtgatggg acgtccgcgg agatctacgc
     2581 gtgtcgactc gagatatcca actagtatgg tgagcgttat caagcctgag atgaagatga
     2641 ggtactacat ggacggaagc gtgaacggac acgagttcac tattgaaggc gaaggtactg
     2701 gaaggcctta cgagggacat caagagatga ctctcagagt gaccatggct gaaggtggac
     2761 ctatgccttt cgctttcgat ctcgtgtctc acgtgttctg ctacggacac agagtgttca
     2821 ctaagtaccc tgaagagatc ccggactact tcaagcaagc tttccctgag ggactctctt
     2881 gggagagatc tttggaattc gaggacggtg gatccgcttc tgtgtctgct catatctctc
     2941 tcaggggcaa caccttctac cacaagagca agtttaccgg ggtgaacttc cctgctgatg
     3001 gaccgatcat gcagaaccag tctgttgatt gggagccgtc tactgagaag atcaccgctt
     3061 ctgatggtgt gctcaagggt gatgtgacca tgtaccttaa gctcgaaggt ggtggaaacc
     3121 acaagtgcca gttcaagact acctacaagg ccgccaaaga aatcctcgag atgcctggtg
     3181 atcactacat cggacatagg ctcgtgagaa agaccgaggg aaacatcact gagcaggttg
     3241 aggatgctgt ggctcattct caattgttat aagcggccat gctagagtcc gcaaaaatca
     3301 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     3361 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     3421 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     3481 aaccaaaatc cagtgacctg caggcatgca agctagctta ctagtgatgc atattctata
     3541 gtgtcaccta aatctgcggc cgcactagtg atatcccgcg gccatggcgg ccgggagcat
     3601 gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     3661 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3721 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3781 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat gggtttctgg agtttaatga
     3841 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
     3901 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
     3961 tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaatggca attaccttat
     4021 ccgcaacttc tttacctatt tccgcccgga tccgggcagg ttctccggcc gcttgggtgg
     4081 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4141 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4201 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4261 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4321 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4381 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4441 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4501 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4561 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4621 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4681 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4741 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4801 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4861 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4921 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4981 ggagttcttc gcccaccccg atccaacact tacgtttgca acgtccaaga gcaaatagac
     5041 cacgaacgcc ggaaggttgc cgcagcgtgt ggattgcgtc tcaattctct cttgcaggaa
     5101 tgcaatgatg aatatgatac tgactatgaa actttgaggg aatactgcct agcaccgtca
     5161 cctcataacg tgcatcatgc atgccctgac aacatggaac atcgctattt ttctgaagaa
     5221 ttatgctcgt tggaggatgt cgcggcaatt gcagctattg ccaaaatcga aatacccctc
     5281 acgcatgcat tcatcaatat tattcatgcg gggaaaggca agattaatcc aactggcaaa
     5341 tcatccagcg tgattggtaa cttcagttcc agcgacttga ttcgttttgg tgctacccac
     5401 gttttcaata aggacgagat ggtggagtaa agaaggagtg cgtcgaagca gatcgttcaa
     5461 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     5521 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     5581 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5641 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5701 atcgaccggc atgcaagctg ataattcaat tcggcgttaa ttcagtacat taaaaacgtc
     5761 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     5821 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     5881 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     5941 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     6001 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     6061 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     6121 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     6181 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     6241 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     6301 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     6361 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     6421 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     6481 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     6541 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     6601 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     6661 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     6721 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     6781 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     6841 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     6901 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     6961 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     7021 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     7081 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     7141 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     7201 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     7261 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     7321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8041 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8101 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8161 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8221 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8281 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8341 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8401 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     8461 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     8521 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8581 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8641 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     8701 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     8761 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     8821 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     8881 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     8941 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9001 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9061 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9121 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9181 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9241 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9301 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     9361 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     9421 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     9481 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9541 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9601 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9661 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     9721 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     9781 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     9841 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     9901 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     9961 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    10021 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    10081 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    10141 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    10201 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    10261 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    10321 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    10381 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    10441 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    10501 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    10561 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    10621 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    10681 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    10741 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    10801 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    10861 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    10921 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    10981 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    11041 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    11101 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    11161 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    11221 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    11281 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    11341 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    11401 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    11461 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    11521 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    11581 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    11641 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    11701 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    11761 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    11821 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    11881 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    11941 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    12001 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    12061 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    12121 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    12181 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
    12241 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    12301 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    12361 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    12421 ctgatccaag ctcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
    12481 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    12541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    12601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    12661 catgattacg aattcgagct cgg
//
