LOCUS       sequence-447320-       11642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G63060.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G63060-mKOk
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1489..1513)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2494..2512)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2495..2512)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2541..2550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2580..2599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2581..2599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2606..2623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2606..2622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2615..2637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2780..2963
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2964..3005,3006..3014,3015..3785)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3045..3064)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3655..3674
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4410..4662
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4755..4779
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5300..6091
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6337..6925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6826..6845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7079..7096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7111..7251
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7177..7196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7337..7359
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7595..7789
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7855..8928)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9357..9986)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11317..11341
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11506..11527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11542..11547,11548..11565,11566..11572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11580..11596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11585..11607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11604..11620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11604..11620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gtcgattcgt ttcagctcaa
      721 ccatcgttgc tgcttcagtc aatttcaaaa gctcgaagaa tttgaacatt aatcgatctc
      781 ggaactgcag attctctgtc cgaagttgcg tgtctgaatc ccaacatgcg cacaagctag
      841 ttttggaagt aaaagaacgg cttgcgaaag attgtactag tctcccactt ggcaaatatg
      901 gaagagatga tgaggatatg atcctctggt ttttgaaaga tcgcagattt tctgttgatg
      961 aagccattgg aaagctaact aaagctatta aatggcgtca tgagttcaag gtagatgagc
     1021 tttctgaaga ttctattaaa gcagctactg acactggaaa agcctatgta catggttttc
     1081 tagatgttaa aggtcggcca gtggttattg tagctcctgc aaagcacatc cccgggctgc
     1141 ttgatccaat agaagatgaa aagctttgtg tgttcttact tgaaaaggct ttaagtaagt
     1201 taccagcagg acaacacaag atacttggga tatttgatct ccgtggattc ggatctcaga
     1261 acgcagatct taagttcttg actttcctgt ttgacgtatt ttactattac tatccaagcc
     1321 gattggatga agtccttttt gtagatgctc cattcatatt tcagccaatt tggcaattca
     1381 ctaaaccgtt ggtgaagcaa tacgcttctc tggtcaagtt ttgctcagca gagaccgtca
     1441 gaaaggagta ctttactgaa gaaacattac catcaaattt ccggagccac ccagctttct
     1501 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     1561 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     1621 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     1681 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     1741 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     1801 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     1861 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     1921 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     1981 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     2041 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     2101 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     2161 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     2221 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2281 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2341 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2401 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     2461 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     2521 gcactagtga tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc
     2581 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2641 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2701 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2761 ggaaattgta agcgttaatg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     2821 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     2881 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     2941 actctcaatc caaataatct gcaatggcaa ttaccttatc cgcaacttct ttacctattt
     3001 ccgcccggat ccgggcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3061 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3121 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3181 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3241 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3301 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3361 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3421 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3481 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     3541 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     3601 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     3661 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3721 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3781 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3841 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3901 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     3961 tccaacactt acgtttgcaa cgtccaagag caaatagacc acgaacgccg gaaggttgcc
     4021 gcagcgtgtg gattgcgtct caattctctc ttgcaggaat gcaatgatga atatgatact
     4081 gactatgaaa ctttgaggga atactgccta gcaccgtcac ctcataacgt gcatcatgca
     4141 tgccctgaca acatggaaca tcgctatttt tctgaagaat tatgctcgtt ggaggatgtc
     4201 gcggcaattg cagctattgc caaaatcgaa atacccctca cgcatgcatt catcaatatt
     4261 attcatgcgg ggaaaggcaa gattaatcca actggcaaat catccagcgt gattggtaac
     4321 ttcagttcca gcgacttgat tcgttttggt gctacccacg ttttcaataa ggacgagatg
     4381 gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa taaagtttct
     4441 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4501 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4561 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4621 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     4681 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     4741 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     4801 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     4861 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     4921 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     4981 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5041 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     5101 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5161 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5281 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5341 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5401 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     5461 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     5521 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     5581 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     5641 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     5701 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     5761 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     5821 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5881 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5941 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6001 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6061 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6121 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6181 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6241 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6301 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6421 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6481 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6541 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6601 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6661 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6721 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6781 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6841 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6901 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6961 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7021 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7081 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7141 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7201 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7261 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7321 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7381 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7441 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7501 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     7561 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     7621 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7681 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7741 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7801 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7861 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7921 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7981 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8041 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     8101 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8161 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8221 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8281 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8341 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8401 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     8461 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     8521 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     8581 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     8641 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8701 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     8761 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8821 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8881 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8941 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9001 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9061 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9121 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9181 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9241 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9301 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9361 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9421 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9481 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9541 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9601 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9661 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9721 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9781 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9841 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9901 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9961 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10021 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10081 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10141 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10201 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10261 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10321 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10381 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10441 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10501 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10561 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10621 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10681 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10741 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10801 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10861 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10921 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10981 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11041 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    11101 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11161 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    11221 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11281 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
    11341 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
    11401 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11581 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
    11641 gg
//
