LOCUS       sequence-447322-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT3G09300.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT3G09300-mKOk
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2074..2098)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3079..3097)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3080..3097)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3165..3184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3166..3184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3191..3208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3191..3207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3200..3222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3365..3548
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3549..3590,3591..3599,3600..4370)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3630..3649)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4240..4259
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4995..5247
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5340..5364
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5885..6676
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6922..7510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7411..7430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7664..7681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7696..7836
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7762..7781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7922..7944
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8180..8374
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8440..9513)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9942..10571)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11902..11926
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12091..12112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12127..12132,12133..12150,12151..12157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12165..12181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12170..12192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12189..12205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12189..12205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggctcctaat gatcctaaaa
      721 aagccgttgg tggcggcgga agtggtttct tcgcctctct tgcttcgtcg atctccaatt
      781 tgggatctgc tatgaccaaa tcagttaatg gtttggttcc ctatgaggga cttgaagtta
      841 tcaatcctga aggaagtaca gatgatgctg aggaggaagc aagcagagga agatggaagc
      901 aagaggatcg agatggctat tggaagatga tgcagaagta cataggatct gatgttacat
      961 caatggtgac ccttcctgtg attatttttg aaccaatgac aatgcttcag aaaatggcgg
     1021 agttgatgga atactcgcat ctgctagaca tggcagacaa aaccgaggac ccttatttgc
     1081 gcatggtgta tgcatcatcg tgggctatat ccgtgtatta tgctttccaa cgtacctgga
     1141 aaccattcaa tccaatcctt ggtgagactt atgagatggc taattacaat ggtgttaact
     1201 tcatatctga acaggtcagc catcacccac caatgagtgc tggtcatgct gaaaatgagc
     1261 attttacgta tgattgtact tcaaaactga aaacaaaatt tttgggcaat tccattgacg
     1321 tttacccagt aggaaggaca cgggtgacac ttaaaagaga tggagttgtt cttgacttgg
     1381 tacctcctct gaccaaagtt cacaacctaa tctttggacg aacttgggtc gattcgcctg
     1441 gggaaatgat catgacaaat cagaccaccg gtgacaaagt cgtgctttac tttcaaccat
     1501 gtggctggtt cggatctggt cgatatgaag tggatggata tgtctataac gcctctgagg
     1561 agcctaagat actcatgact ggcaaatgga acgagtccat gagttatcag ccgtgtgatg
     1621 gtgaaggtga acctctccca ggcaccgaac tgaaagaggt atggaaactc gctgatgtgc
     1681 caaaggatga caaatatcaa tacactcact ttgctcacaa gattaatagc ttcgacactg
     1741 ccccgaaaaa gctgttgccc tctgattcac ggttacgacc tgatagatac gcacttgaga
     1801 tgggcgacat gtccaaatca ggctatgaga agagcagcat ggaagagaga cagagagctg
     1861 aaaagagaac ccgcgaagag aaaggccaag cctttactcc aaaatggttc gatgtaacgg
     1921 aagaagtcac tgctacacca tggggtgatc tggaagttta ccaattcaat ggaaagtact
     1981 cggaacatcg tgcagctgcg gataactctg aagataacac cgaccctaag tcgatccaat
     2041 tcaacccatg gcaattccaa gatctgtcta ctcacccagc tttcttgtac aaagtggtga
     2101 tgggacgtcc gcggagatct acgcgtgtcg actcgagata tccaactagt atggtgagcg
     2161 ttatcaagcc tgagatgaag atgaggtact acatggacgg aagcgtgaac ggacacgagt
     2221 tcactattga aggcgaaggt actggaaggc cttacgaggg acatcaagag atgactctca
     2281 gagtgaccat ggctgaaggt ggacctatgc ctttcgcttt cgatctcgtg tctcacgtgt
     2341 tctgctacgg acacagagtg ttcactaagt accctgaaga gatcccggac tacttcaagc
     2401 aagctttccc tgagggactc tcttgggaga gatctttgga attcgaggac ggtggatccg
     2461 cttctgtgtc tgctcatatc tctctcaggg gcaacacctt ctaccacaag agcaagttta
     2521 ccggggtgaa cttccctgct gatggaccga tcatgcagaa ccagtctgtt gattgggagc
     2581 cgtctactga gaagatcacc gcttctgatg gtgtgctcaa gggtgatgtg accatgtacc
     2641 ttaagctcga aggtggtgga aaccacaagt gccagttcaa gactacctac aaggccgcca
     2701 aagaaatcct cgagatgcct ggtgatcact acatcggaca taggctcgtg agaaagaccg
     2761 agggaaacat cactgagcag gttgaggatg ctgtggctca ttctcaattg ttataagcgg
     2821 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     2881 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     2941 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     3001 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagctag
     3061 cttactagtg atgcatattc tatagtgtca cctaaatctg cggccgcact agtgatatcc
     3121 cgcggccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt
     3181 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     3361 taatgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
     3421 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
     3481 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caatccaaat
     3541 aatctgcaat ggcaattacc ttatccgcaa cttctttacc tatttccgcc cggatccggg
     3601 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3661 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3721 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3781 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3841 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3901 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3961 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4021 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4081 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     4141 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4201 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4261 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4321 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4381 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4441 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4501 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgatccaa cacttacgtt
     4561 tgcaacgtcc aagagcaaat agaccacgaa cgccggaagg ttgccgcagc gtgtggattg
     4621 cgtctcaatt ctctcttgca ggaatgcaat gatgaatatg atactgacta tgaaactttg
     4681 agggaatact gcctagcacc gtcacctcat aacgtgcatc atgcatgccc tgacaacatg
     4741 gaacatcgct atttttctga agaattatgc tcgttggagg atgtcgcggc aattgcagct
     4801 attgccaaaa tcgaaatacc cctcacgcat gcattcatca atattattca tgcggggaaa
     4861 ggcaagatta atccaactgg caaatcatcc agcgtgattg gtaacttcag ttccagcgac
     4921 ttgattcgtt ttggtgctac ccacgttttc aataaggacg agatggtgga gtaaagaagg
     4981 agtgcgtcga agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     5041 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     5101 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     5161 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     5221 gcgcggtgtc atctatgtta ctagatcgac cggcatgcaa gctgataatt caattcggcg
     5281 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     5341 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     5401 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     5461 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     5521 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     5581 agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt
     5641 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     5701 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     5761 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     5821 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     5881 catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     5941 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     6001 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     6061 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     6121 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     6181 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     6241 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     6301 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     6361 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     6421 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     6481 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     6541 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     6601 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     6661 ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc
     6721 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     6781 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     6841 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7681 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7741 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     7801 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7861 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7921 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     7981 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     8041 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     8101 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     8161 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     8221 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     8281 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     8341 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     8401 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     8461 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     8521 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     8581 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     8641 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     8701 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     8761 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     8821 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     8881 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     8941 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     9001 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     9061 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     9121 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     9181 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     9241 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     9301 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     9361 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     9421 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     9481 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     9541 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
     9601 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     9661 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     9721 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     9781 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     9841 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     9901 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     9961 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
    10021 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
    10081 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
    10141 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
    10201 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
    10261 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
    10321 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
    10381 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
    10441 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
    10501 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
    10561 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
    10621 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
    10681 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
    10741 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
    10801 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    10861 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    10921 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    10981 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    11041 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    11101 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    11161 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    11221 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    11281 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    11341 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    11401 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    11461 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    11521 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    11581 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    11641 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    11701 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtacttta
    11761 aagtacttta aagtacttta aagtactttg atcccgaggg gaaccctgtg gttggcatgc
    11821 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    11881 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    11941 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc caatacgcaa
    12001 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    12061 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    12121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    12181 atttcacaca ggaaacagct atgacatgat tacgaattcg agctcgg
//
