LOCUS       sequence-447324-       12143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT2G20590.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT2G20590-mKOk
FEATURES             Location/Qualifiers
     source          1..12143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1990..2014)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2995..3013)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2996..3013)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3042..3051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3081..3100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3082..3100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3107..3124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3107..3123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3116..3138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3281..3464
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3465..3506,3507..3515,3516..4286)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3546..3565)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4156..4175
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4911..5163
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5256..5280
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5801..6592
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6838..7426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7327..7346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7580..7597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7612..7752
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7678..7697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7838..7860
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8096..8290
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8356..9429)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9858..10487)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11818..11842
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12007..12028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12043..12048,12049..12066,12067..12073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12081..12097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12086..12108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12105..12121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12105..12121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatgga gtctacgcct ccttatcacc
      721 gatccaacac taaatcagct tcgcgtctcc aagattcttc gaatcctcca aatctctctc
      781 ttgatttggt tctgtcgtct cccaatactc caaacccatc atcacctgtt cctcttcgcg
      841 atatcctcct cttacctcct tctccacttc gaaaatcgag gacccgttta tccgatcgac
      901 tcgaaatgac ttctgaagat gctatggcgg tggttcggaa acggggcaaa gggaaaggag
      961 ggcaaaagag tcttttagca tctccgagga atccccggag atcgaggcga agatctgaag
     1021 ctgtggaaga gaaagaagct aatttggtta tcgaagaaat cgtaaagctt cctcctagaa
     1081 agcggaagac taatggtcga cctaagaagg ataagcaaag ttcagctcct cctctttgtt
     1141 cttcctcaga tcttccaaat acatgtcaga gcgatttgaa tctgattgga gagattataa
     1201 gtgatttggt aatgtggaga gatgttgcga aatcgactct ctggtttggt tttggatgtt
     1261 tgtctttcct atcttcttgc tttgccaaag gagtcaactt tagcgttttc tcggcggttt
     1321 ccaatctcgg acttgtgtta ctttgcgggt ccttcttgtc aaacactctt tgtcaaagga
     1381 aaaatgaaga cacaaagaga gcgttccatg tgagtgaaga tgatgtcttg cgctcagcta
     1441 gacgagtgct tcctgcaacc aactttttta tctcgaagac tagtgagctt ttttcaggag
     1501 aaccatcaat gacactcaaa gtgacaccat ttctgctact tggagctgag tatggtcatc
     1561 tcataacact gtggaggcta tctgcatttg gtttcttcct cagtttcacc atcccaaagc
     1621 tgtattcatg ttacacgcat cagattagcc aaaaagttga aagagtgaaa acgagaatag
     1681 gtgaagcatg gggagtctgc tcacacaaga agatcttggc tggctctgcg gtgacagcgt
     1741 tttggaactt gacgagcata agaacccgaa tatttgcagt ttttatcatc ctggtgatat
     1801 ttcggtatag gagacaaaat ctacagctaa ctcccgagga agttgagcct gttgagaatg
     1861 agcaagaaga agaaacactc ccgcaggaag aagaaacagt tccacaagaa gaagaaacag
     1921 ttccacaaga agaagaacag acacagccat cagaagagcg agctttagtg gtggtggttg
     1981 cagaaactga cccagctttc ttgtacaaag tggtgatggg acgtccgcgg agatctacgc
     2041 gtgtcgactc gagatatcca actagtatgg tgagcgttat caagcctgag atgaagatga
     2101 ggtactacat ggacggaagc gtgaacggac acgagttcac tattgaaggc gaaggtactg
     2161 gaaggcctta cgagggacat caagagatga ctctcagagt gaccatggct gaaggtggac
     2221 ctatgccttt cgctttcgat ctcgtgtctc acgtgttctg ctacggacac agagtgttca
     2281 ctaagtaccc tgaagagatc ccggactact tcaagcaagc tttccctgag ggactctctt
     2341 gggagagatc tttggaattc gaggacggtg gatccgcttc tgtgtctgct catatctctc
     2401 tcaggggcaa caccttctac cacaagagca agtttaccgg ggtgaacttc cctgctgatg
     2461 gaccgatcat gcagaaccag tctgttgatt gggagccgtc tactgagaag atcaccgctt
     2521 ctgatggtgt gctcaagggt gatgtgacca tgtaccttaa gctcgaaggt ggtggaaacc
     2581 acaagtgcca gttcaagact acctacaagg ccgccaaaga aatcctcgag atgcctggtg
     2641 atcactacat cggacatagg ctcgtgagaa agaccgaggg aaacatcact gagcaggttg
     2701 aggatgctgt ggctcattct caattgttat aagcggccat gctagagtcc gcaaaaatca
     2761 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     2821 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     2881 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2941 aaccaaaatc cagtgacctg caggcatgca agctagctta ctagtgatgc atattctata
     3001 gtgtcaccta aatctgcggc cgcactagtg atatcccgcg gccatggcgg ccgggagcat
     3061 gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     3121 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3181 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3241 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat gggtttctgg agtttaatga
     3301 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
     3361 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
     3421 tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaatggca attaccttat
     3481 ccgcaacttc tttacctatt tccgcccgga tccgggcagg ttctccggcc gcttgggtgg
     3541 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3601 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3661 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3721 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3781 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3841 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3901 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3961 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4021 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4081 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4141 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4201 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4261 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4321 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4381 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4441 ggagttcttc gcccaccccg atccaacact tacgtttgca acgtccaaga gcaaatagac
     4501 cacgaacgcc ggaaggttgc cgcagcgtgt ggattgcgtc tcaattctct cttgcaggaa
     4561 tgcaatgatg aatatgatac tgactatgaa actttgaggg aatactgcct agcaccgtca
     4621 cctcataacg tgcatcatgc atgccctgac aacatggaac atcgctattt ttctgaagaa
     4681 ttatgctcgt tggaggatgt cgcggcaatt gcagctattg ccaaaatcga aatacccctc
     4741 acgcatgcat tcatcaatat tattcatgcg gggaaaggca agattaatcc aactggcaaa
     4801 tcatccagcg tgattggtaa cttcagttcc agcgacttga ttcgttttgg tgctacccac
     4861 gttttcaata aggacgagat ggtggagtaa agaaggagtg cgtcgaagca gatcgttcaa
     4921 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     4981 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     5041 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5101 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5161 atcgaccggc atgcaagctg ataattcaat tcggcgttaa ttcagtacat taaaaacgtc
     5221 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     5281 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     5341 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     5401 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     5461 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     5521 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     5581 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     5641 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     5701 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     5761 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     5821 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     5881 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     5941 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     6001 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     6061 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     6121 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     6181 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     6241 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     6301 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     6361 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     6421 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     6481 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     6541 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     6601 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     6661 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     6721 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     6781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6961 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7201 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7501 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7561 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     7621 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     7681 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     7741 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7801 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7861 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     7921 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     7981 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8041 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8101 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     8161 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     8221 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     8281 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     8341 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     8401 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     8461 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     8521 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     8581 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     8641 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     8701 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     8761 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     8821 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     8881 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     8941 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9001 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9061 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9121 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     9181 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     9241 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     9301 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     9361 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     9421 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
     9481 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     9541 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     9601 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     9661 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     9721 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     9781 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     9841 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     9901 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     9961 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    10021 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    10081 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    10141 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    10201 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    10261 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    10321 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    10381 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    10441 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    10501 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    10561 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    10621 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    10681 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    10741 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    10801 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    10861 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    10921 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    10981 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    11041 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    11101 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    11161 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    11221 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    11281 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    11341 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    11401 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    11461 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    11521 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    11581 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    11641 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
    11701 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    11761 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    11821 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    11881 ctgatccaag ctcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
    11941 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    12001 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    12061 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    12121 catgattacg aattcgagct cgg
//
