LOCUS       sequence-447325-       14975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be 
            cloned in BsaI site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Karmakar S, Panda D, Panda S, Dash M, Saha R, Das P, Avinash SP, 
            Shih J, Yang Y, Nayak AK, Baig MJ, Molla KA
  TITLE     A miniature alternative to Cas9 and Cas12: Transposon-associated 
            TnpB mediates targeted genome editing in plants.
  JOURNAL   Plant Biotechnol J. 2024 Jun 28. doi: 10.1111/pbi.14416.
  PUBMED    38940895
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2024 Jun 28. doi: 10.1111/pbi.14416."
COMMENT     Sequence Label: pKb-TnpB2
FEATURES             Location/Qualifiers
     source          1..14975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..264
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(273..290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(273..289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(282..304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    492..516
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1817..2446
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2875..3948
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4014..4208
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4444..4466)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4552..4692
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4607..4626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4707..4724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4878..5466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4958..4977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5553..6347)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6772..6796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6874..7048
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7089..8114)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8181..8857)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8224..8244)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9048..9069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9084..9089,9090..9107,9108..9114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9122..9138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9127..9149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9146..9162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9146..9162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9186..11178
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     ncRNA           11481..11548
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      11549..11801
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11817..13526
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          12565..13526
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    13537..13561
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      13567..13576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13579..13599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14848..14895
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(14907..14931)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             14942..14965
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
       61 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
      121 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
      181 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
      241 cggtgtcatc tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac
      301 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      421 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
      481 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
      541 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
      601 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
      661 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      721 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      781 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      841 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      901 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      961 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     1021 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     1081 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     1141 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     1201 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     1261 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     1321 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     1381 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     1441 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     1501 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     1561 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1621 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1681 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1741 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1801 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1861 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1921 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1981 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     2041 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     2101 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     2161 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     2221 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     2281 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     2341 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     2401 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     2461 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     2521 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     2581 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2641 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2701 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2761 cgacgccgtg gaatgcccca tgtgttgagg aacgggcggt tggccaggcg taagcggctg
     2821 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     2881 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     2941 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     3001 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     3061 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     3121 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     3181 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     3241 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     3301 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     3361 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     3421 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     3481 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     3541 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3601 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3661 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3721 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3781 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     3841 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     3901 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     3961 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagcactctt tcctgtgaat
     4021 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     4081 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     4141 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     4201 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     4261 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     4321 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     4381 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     4441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     4561 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4621 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4681 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4741 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4801 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4861 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4921 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4981 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5041 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5101 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5161 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5221 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5281 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5341 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5401 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5461 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5521 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     5581 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5641 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     5701 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     5761 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     5821 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     5881 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     5941 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     6001 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     6061 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     6121 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     6181 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     6241 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     6301 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     6361 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     6421 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     6481 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     6541 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     6601 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     6661 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     6721 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     6781 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     6841 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     6901 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     6961 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     7021 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
     7081 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     7141 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     7201 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     7261 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     7321 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     7381 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     7441 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     7501 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     7561 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     7621 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     7681 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     7741 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
     7801 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
     7861 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     7921 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     7981 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     8041 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     8101 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
     8161 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
     8221 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
     8281 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     8341 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     8401 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     8461 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     8521 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     8581 acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg
     8641 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     8701 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     8761 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     8821 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     8881 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
     8941 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9001 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9061 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9121 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gccaagcttg
     9181 catgcctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat
     9241 gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt
     9301 atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct atagtactac
     9361 aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca
     9421 attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc
     9481 tttttttttg caaatagctt cacctatata atacttcatc cattttatta gtacatccat
     9541 ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct attttattct
     9601 attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt
     9661 agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt
     9721 aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc
     9781 gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
     9841 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
     9901 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
     9961 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
    10021 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
    10081 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
    10141 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
    10201 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
    10261 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
    10321 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
    10381 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
    10441 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
    10501 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
    10561 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat
    10621 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
    10681 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
    10741 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
    10801 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
    10861 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
    10921 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
    10981 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
    11041 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
    11101 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
    11161 ttggtgttac ttctgcagcc ctaggattct tgaatcctga tgagtccgtg aggacgaaac
    11221 gagctagctc gtcgattcaa gaatcccgaa gtgaagaatc ttgccgtccg tacatggact
    11281 tgcccgaact gtggggaaac ccatgaccga gacgagaacg ctgcgctgaa cattcggcgt
    11341 gaagcgttgg tggctgcggg aatctcagac accttaaacg ctcatggagg ctatgtcaga
    11401 cctgcttcgg cgggcaatgg tctgcgaagt gagaatcacg cgactttagt cgtgtgaggt
    11461 tcaaggagac cgaggtctcg ggccggcatg gtcccagcct cctcgctggc gccggctggg
    11521 caacatgctt cggcatggcg aatgggacga tcgttcaaac atttggcaat aaagtttctt
    11581 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
    11641 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
    11701 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
    11761 ggataaatta tcgcgcgcgg tgtcatctat gttactagat ccctgcaggt ccacaaattc
    11821 gggtcaaggc ggaagccagc gcgccacccc acgtcagcaa atacggaggc gcggggttga
    11881 cggcgtcacc cggtcctaac ggcgaccaac aaaccagcca gaagaaatta cagtaaaaaa
    11941 aaagtaaatt gcactttgat ccacctttta ttacctaagt ctcaatttgg atcaccctta
    12001 aacctatctt ttcaatttgg gccgggttgt ggtttggact accatgaaca acttttcgtc
    12061 atgtctaact tccctttcag caaacatatg aaccatatat agaggagatc ggccgtatac
    12121 tagagctgat gtgtttaagg tcgttgattg cacgagaaaa aaaaatccaa atcgcaacaa
    12181 tagcaaattt atctggttca aagtgaaaag atatgtttaa aggtagtcca aagtaaaact
    12241 tatagataat aaaatgtggt ccaaagcgta attcactcaa aaaaaatcaa cgagacgtgt
    12301 accaaacgga gacaaacggc atcttctcga aatttcccaa ccgctcgctc gcccgcctcg
    12361 tcttcccgga aaccgcggtg gtttcagcgt ggcggattct ccaagcagac ggagacgtca
    12421 cggcacggga ctcctcccac cacccaaccg ccataaatac cagccccctc atctcctctc
    12481 ctcgcatcag ctccaccccc gaaaaatttc tccccaatct cgcgaggctc tcgtcgtcga
    12541 atcgaatcct ctcgcgtcct caaggtacgc tgcttctcct ctcctcgctt cgtttcgatt
    12601 cgatttcgga cgggtgaggt tgttttgttg ctagatccga ttggtggtta gggttgtcga
    12661 tgtgattatc gtgagatgtt taggggttgt agatctgatg gttgtgattt gggcacggtt
    12721 ggttcgatag gtggaatcgt ggttaggttt tgggattgga tgttggttct gatgattggg
    12781 gggaattttt acggttagat gaattgttgg atgattcgat tggggaaatc ggtgtagatc
    12841 tgttggggaa ttgtggaact agtcatgcct gagtgattgg tgcgatttgt agcgtgttcc
    12901 atcttgtagg ccttgttgcg agcatgttca gatctactgt tccgctcttg attgagttat
    12961 tggtgccatg ggttggtgca aacacaggct ttaatatgtt atatctgttt tgtgtttgat
    13021 gtagatctgt agggtagttc ttcttagaca tggttcaatt atgtagcttg tgcgtttcga
    13081 tttgatttca tatgttcaca gattagataa tgatgaactc ttttaattaa ttgtcaatgg
    13141 taaataggaa gtcttgtcgc tatatctgtc ataatgatct catgttacta tctgccagta
    13201 atttatgcta agaactatat tagaatatca tgttacaatc tgtagtaata tcatgttaca
    13261 atctgtagtt catctatata atctattgtg gtaatttctt tttactatct gtgtgaagat
    13321 tattgccact agttcattct acttatttct gaagttcagg atacgtgtgc tgttactacc
    13381 tatctgaata catgtgtgat gtgcctgtta ctatcttttt gaatacatgt atgttctgtt
    13441 ggaatatgtt tgctgtttga tccgttgttg tgtccttaat cttgtgctag ttcttaccct
    13501 atctgtttgg tgattatttc ttgcagatag ttatcaacaa gtttgtacaa aaaagcaggc
    13561 ttcgaagcca ccatggctcc taagaagaag cggaaggttg gtattcacgg ggtgcctgcg
    13621 gctatgatta ggaacaaggc cttcgtcgtc agattgtacc ctaatgcagc gcagactgaa
    13681 cttatcaaca gaacgttagg aagcgcgcgt tttgtttaca accacttcct ggcacgacgc
    13741 attgccgcct ataaggagag tggaaaggga cttacttatg ggcagacgtc atcagaatta
    13801 accctcctca agcaggcgga ggagacgtcc tggctgagcg aagtagacaa gttcgccctc
    13861 caaaattccc tgaagaatct cgaaaccgcg tacaagaact tcttccgcac tgttaagcaa
    13921 tctggcaaga aagtgggttt tccgcggttc aggaagaagc gtacgggtga gtcctacaga
    13981 acccagttta ctaacaataa cattcagatc ggagagggtc gtctaaagct acccaagttg
    14041 ggttgggtca aaacgaaagg ccaacaagat attcaaggga agattctcaa tgtcacagta
    14101 cggcggattc atgaaggcca ctacgaggcc agcgtccttt gtgaggtcga gatcccgtat
    14161 ctccctgcgg cccccaaatt tgctgccggc gtggatgtgg gcatcaagga ttttgcgata
    14221 gttaccgatg gcgtcaggtt caagcacgag caaaatccaa agtactaccg ctccaccctt
    14281 aagcgcttga gaaaagctca gcagactttg tccaggagga aaaagggctc ggcgcgttat
    14341 ggaaaagcca agacaaagct ggcaaggata cacaaaagga tcgtgaacaa acggcaggac
    14401 ttcctccaca agctaacaac atctttggtt agagaatatg aaataatcgg gactgagcac
    14461 ctgaaaccag acaacatgcg caaaaatcgc cgcctcgcgc tgtcgatctc tgatgcaggg
    14521 tggggcgagt tcattcgaca gctggagtac aaagcagctt ggtacggcag gctggtatcg
    14581 aaggtttctc catactttcc gagttcacag ttgtgccatg attgcggctt caaaaatccg
    14641 gaggtgaaga atcttgccgt gcggacatgg acatgtccca actgcggtga aacacatgac
    14701 agagacgaga acgctgccct gaacataagg cgggaagcac tcgtggcggc cggaatcagt
    14761 gacaccctca atgctcatgg gggctatgtg aggccggcta gcgctgggaa tggtcttcgc
    14821 tcagagaatc atgctacctt ggttgtgaag cgtcctgctg ccaccaaaaa ggccggacag
    14881 gctaagaaaa agaagtgaat atctagaccc agctttcttg tacaaagtgg ttgataacag
    14941 cgactacaag gatgacgatg acaaggctta gagct
//
