LOCUS       sequence-447326-       11513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT2G38360.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT2G38360-mKOk
FEATURES             Location/Qualifiers
     source          1..11513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1360..1384)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2365..2383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2366..2383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2412..2421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2451..2470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2452..2470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2477..2494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2477..2493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2486..2508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2651..2834
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2835..2876,2877..2885,2886..3656)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2916..2935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3526..3545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4281..4533
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4626..4650
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5171..5962
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6208..6796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6697..6716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6950..6967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6982..7122
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7048..7067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7208..7230
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7466..7660
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7726..8799)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9228..9857)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11188..11212
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11377..11398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11413..11418,11419..11436,11437..11443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11451..11467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11456..11478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11475..11491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11475..11491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggcttcctca gcaccaccag
      721 tcctcccaat ctccaatccc caaaccgttc catccgccgc tccttcctcc gtcgaatctc
      781 aaccaccgat cgctactcca gctttccgca atttcatcaa tcagatcacc gaaaccgtta
      841 aaaacggtct ctctaagcgt cgtccttggg ctgagctcgc ggatcgttcc gcattatcca
      901 aacctgagtc gatctccgac gctgctgttc gtatccgcaa gaattactcg tatttcaagg
      961 ttaattacct caccgtcgct acagcgatcg tcggattctc tctcgtcact catcctttct
     1021 ctctcgtctt cctcctctgt ctcctcgcgt cgtggctctt cctctacttg ttccgtccca
     1081 cggatcagcc gatcgttcta ttcggtcgta cattctccga tcgtgagacg ttagggtgtt
     1141 tgatcttgtt cagtatcttt gtgatcttcc tcaccgatgt tggatctgtt ctggtttcgg
     1201 ctatgatgat tggtgtcgcg ttgatctgtg ctcatggtgc ttttagagct ccggaagatc
     1261 ttttccttga tgagcaagag cctgctgcta ccggattcct ctctttcctc ggtggtgccg
     1321 cctcttcagc cgcgcctgct gttatcgccg ctcgcgtgca cccagctttc ttgtacaaag
     1381 tggtgatggg acgtccgcgg agatctacgc gtgtcgactc gagatatcca actagtatgg
     1441 tgagcgttat caagcctgag atgaagatga ggtactacat ggacggaagc gtgaacggac
     1501 acgagttcac tattgaaggc gaaggtactg gaaggcctta cgagggacat caagagatga
     1561 ctctcagagt gaccatggct gaaggtggac ctatgccttt cgctttcgat ctcgtgtctc
     1621 acgtgttctg ctacggacac agagtgttca ctaagtaccc tgaagagatc ccggactact
     1681 tcaagcaagc tttccctgag ggactctctt gggagagatc tttggaattc gaggacggtg
     1741 gatccgcttc tgtgtctgct catatctctc tcaggggcaa caccttctac cacaagagca
     1801 agtttaccgg ggtgaacttc cctgctgatg gaccgatcat gcagaaccag tctgttgatt
     1861 gggagccgtc tactgagaag atcaccgctt ctgatggtgt gctcaagggt gatgtgacca
     1921 tgtaccttaa gctcgaaggt ggtggaaacc acaagtgcca gttcaagact acctacaagg
     1981 ccgccaaaga aatcctcgag atgcctggtg atcactacat cggacatagg ctcgtgagaa
     2041 agaccgaggg aaacatcact gagcaggttg aggatgctgt ggctcattct caattgttat
     2101 aagcggccat gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc
     2161 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag
     2221 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     2281 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgca
     2341 agctagctta ctagtgatgc atattctata gtgtcaccta aatctgcggc cgcactagtg
     2401 atatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg
     2461 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2521 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2581 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
     2641 aagcgttaat gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg
     2701 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
     2761 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat
     2821 ccaaataatc tgcaatggca attaccttat ccgcaacttc tttacctatt tccgcccgga
     2881 tccgggcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     2941 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     3001 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     3061 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     3121 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     3181 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     3241 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     3301 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     3361 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     3421 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     3481 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     3541 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     3601 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     3661 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     3721 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     3781 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg atccaacact
     3841 tacgtttgca acgtccaaga gcaaatagac cacgaacgcc ggaaggttgc cgcagcgtgt
     3901 ggattgcgtc tcaattctct cttgcaggaa tgcaatgatg aatatgatac tgactatgaa
     3961 actttgaggg aatactgcct agcaccgtca cctcataacg tgcatcatgc atgccctgac
     4021 aacatggaac atcgctattt ttctgaagaa ttatgctcgt tggaggatgt cgcggcaatt
     4081 gcagctattg ccaaaatcga aatacccctc acgcatgcat tcatcaatat tattcatgcg
     4141 gggaaaggca agattaatcc aactggcaaa tcatccagcg tgattggtaa cttcagttcc
     4201 agcgacttga ttcgttttgg tgctacccac gttttcaata aggacgagat ggtggagtaa
     4261 agaaggagtg cgtcgaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
     4321 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     4381 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     4441 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     4501 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat
     4561 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     4621 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     4681 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     4741 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     4801 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     4861 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     4921 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     4981 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     5041 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     5101 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     5161 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     5221 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     5281 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     5341 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     5401 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     5461 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     5521 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     5581 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     5641 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     5701 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     5761 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     5821 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     5881 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     5941 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     6001 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     6061 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     6121 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6841 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6901 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6961 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7021 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7081 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     7141 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7201 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     7261 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     7321 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     7381 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     7441 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     7501 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     7561 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     7621 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     7681 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     7741 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     7801 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     7861 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     7921 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     7981 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     8041 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     8101 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     8161 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     8221 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     8281 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     8341 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     8401 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     8461 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     8521 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     8581 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     8641 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     8701 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     8761 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     8821 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     8881 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     8941 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     9001 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     9061 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     9121 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     9181 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     9241 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     9301 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     9361 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     9421 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     9481 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     9541 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     9601 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     9661 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     9721 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     9781 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     9841 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     9901 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     9961 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    10021 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    10081 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    10141 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    10201 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    10261 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    10321 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    10381 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    10441 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    10501 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    10561 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    10621 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    10681 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    10741 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    10801 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    10861 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    10921 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    10981 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    11041 actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg
    11101 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
    11161 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
    11221 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagccaat
    11281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    11341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    11401 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    11461 ataacaattt cacacaggaa acagctatga catgattacg aattcgagct cgg
//
