LOCUS       sequence-447327-       13202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT1G73430.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT1G73430-mKOk
FEATURES             Location/Qualifiers
     source          1..13202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3049..3073)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4054..4072)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4055..4072)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      4101..4110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4140..4159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4141..4159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4166..4183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4166..4182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4175..4197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        4340..4523
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(4524..4565,4566..4574,4575..5345)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4605..4624)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5215..5234
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5970..6222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6315..6339
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6860..7651
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7897..8485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8386..8405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8639..8656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8671..8811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8737..8756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8897..8919
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9155..9349
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9415..10488)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10917..11546)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12877..12901
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13066..13087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13102..13107,13108..13125,13126..13132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13140..13156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13145..13167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13164..13180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13164..13180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatggc gactaaggcg gcttcgtctt
      721 ccagcctccc caaatctgga gccatttcca aaggctacaa tttcgcttcg acttgggaac
      781 agagcgctcc tttgacagag cagcaacaag cagctattgt ttctttatcc cacgcagtcg
      841 ctgagcggcc atttcctgcc aatcttgtac atgagcatgt tcacaggcct gagaatggct
      901 tgtctgcctc cgtggaggac acacatttgg gagattctgg agcaattgaa gctgttttgg
      961 ttaatacaaa tcagttctac aaatggttta ctgatcttga atcagcgatg aagtctgaga
     1021 cagaggaaaa gtatcggcac tatgttagta ctttaactga gcgcatccag acgtgtgata
     1081 atatacttca ccaggttgat gaaacacttg acctatttaa cgaactgcag ctacaacatc
     1141 aaggagtaac aacaaagact aaaactcttc atgacgcatg tgaccgactg ttaatggaga
     1201 agcagaaatt gatggaattt gcagaagctc taaggagtaa gctcaactat tttgatgaac
     1261 tggagaatgt atcctctaat ttttattcac caaatatgaa tgtatcaaac tcaaactttc
     1321 tcccactact gaaacggctg gatgagtgca tatcgtacat cgaagataat cctcaatatg
     1381 cggagtcgag tgtttatttg ctcaaatttc gtcaactcca gtcacgggcc ttgggcatga
     1441 ttcgaactta catccttgca gtgcttaaaa cggctgcttc tcaggttcag gcagcatttc
     1501 gtgggacagg tggaaacaaa acatctgtct cggagggtgt cgaggcatct gttatatatg
     1561 ttcgcttcaa ggctgctgca aatgagctta aacctgtatt ggaggagatt gagagtaggt
     1621 cagcaagaaa ggaatatgtc caaatccttg cagaatgcca cagactatac tgtgaacaac
     1681 ggctctcgct tgtcaaaggc atagtgcacc aacgagtatc tgatttcgcc aagaaagagg
     1741 cactgccatc tttaactaga tcgggttgtg catatctgat gcaggtttgt catatggagc
     1801 atcaactgtt tactcatttc tttccagcat cgtcagaaga agtttcgagt ctggcacctt
     1861 tagtggatcc tttgtctacg tacttgtatg acattttacg cccaaaatta attcacgaag
     1921 caaatattga tcttctatgt gagctcgttc atatccttaa agttgaagtc ctgggagacc
     1981 agtcagctag acagagtgaa cccctggccg gactgaggcc tacattacaa agaattttgg
     2041 ccgatgtaaa tgagagattg actttccgtg ccagaacata tattagagat gagattgcaa
     2101 attatacccc ctctgatgaa gatctggatt atcctgcaaa gctggaagga tctcctaata
     2161 caacatctga aactgatctc agggatgatg aaaacgctga tgtgtttaag acttggtatc
     2221 cacctcttga aaagactctc tcttgtctat ctaaattata tcgttgcttg gagcaggccg
     2281 tcttcactgg attagcacag gaagctgtag aagtttgctc actgtcaatt caaaaagcca
     2341 gcaagcttat cattaagcga tcaactacaa tggatggcca gcttttcctt atcaagcatc
     2401 tccttatctt gagagaacag attgcccctt ttgacataga attttcagta acccataagg
     2461 agcttgattt ctctcattta ctggaacact tgaggaggat acttagaggt caagcgtcac
     2521 tttttgactg gtctaggtca acctctctag caaggaccct gtcacctcga gttttggaga
     2581 gtcaaataga tgccaaaaag gaactggaaa aatgtcttaa gacaacttgt gaggagttca
     2641 tcatgtcagt aactaagcta gttgtggatc ctatgctttc atttgtgacg aaggtcacag
     2701 ccataaaagt tgctctttcc tccggaactc agaatcacaa agttgattct gtcatggcta
     2761 agccgctgaa ggaacaagct ttcgcgacac cagataaggt ggttgaactt gttcaaaagg
     2821 tatacgctgc gatacaacaa gagttgcttc cgattttagc aaaaatgaaa ctctatttgc
     2881 aaaacccatc aacaaggacc atacttttca agccgatcaa gacaaatata gtggaagctc
     2941 atacgcaagt ggagtctttg ctcaaagctg agtactcagc tgaagaacaa gccaatatta
     3001 atatgatctc cattcaggat ttgcaaaccc aactcgacaa tttccttgac ccagctttct
     3061 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     3121 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     3181 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     3241 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     3301 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     3361 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     3421 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     3481 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     3541 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     3601 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     3661 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     3721 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     3781 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     3841 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     3901 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     3961 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     4021 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     4081 gcactagtga tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc
     4141 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4201 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4261 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4321 ggaaattgta agcgttaatg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     4381 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     4441 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     4501 actctcaatc caaataatct gcaatggcaa ttaccttatc cgcaacttct ttacctattt
     4561 ccgcccggat ccgggcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4621 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4681 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4741 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4801 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4861 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4921 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4981 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5041 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5101 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5161 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5221 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5281 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5341 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     5521 tccaacactt acgtttgcaa cgtccaagag caaatagacc acgaacgccg gaaggttgcc
     5581 gcagcgtgtg gattgcgtct caattctctc ttgcaggaat gcaatgatga atatgatact
     5641 gactatgaaa ctttgaggga atactgccta gcaccgtcac ctcataacgt gcatcatgca
     5701 tgccctgaca acatggaaca tcgctatttt tctgaagaat tatgctcgtt ggaggatgtc
     5761 gcggcaattg cagctattgc caaaatcgaa atacccctca cgcatgcatt catcaatatt
     5821 attcatgcgg ggaaaggcaa gattaatcca actggcaaat catccagcgt gattggtaac
     5881 ttcagttcca gcgacttgat tcgttttggt gctacccacg ttttcaataa ggacgagatg
     5941 gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa taaagtttct
     6001 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     6061 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     6121 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     6181 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     6241 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     6301 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     6361 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     6421 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     6481 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     6541 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     6601 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     6661 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     6721 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     6781 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     6841 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     6901 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6961 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     7021 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     7081 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     7141 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     7201 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     7261 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     7321 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     7381 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     7441 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     7501 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     7561 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     7621 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     7681 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     7741 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     7801 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8041 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8641 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8701 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     8761 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8821 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8881 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8941 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     9001 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     9061 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     9121 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     9181 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     9241 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     9301 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     9361 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     9421 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     9481 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     9541 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     9601 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     9661 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     9721 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     9781 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     9841 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     9901 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     9961 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    10021 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    10081 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    10141 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    10201 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    10261 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    10321 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    10381 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    10441 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    10501 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
    10561 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    10621 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    10681 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    10741 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    10801 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    10861 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    10921 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    10981 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    11041 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    11101 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    11161 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    11221 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    11281 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    11341 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    11401 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    11461 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    11521 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    11581 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    11641 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    11701 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    11761 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    11821 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    11881 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    11941 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    12001 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    12061 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    12121 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    12181 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    12241 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    12301 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    12361 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    12421 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    12481 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    12541 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    12601 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    12661 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    12721 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    12781 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    12841 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
    12901 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
    12961 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    13021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    13081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    13141 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
    13201 gg
//
