LOCUS       sequence-447328-       12182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT4G11790.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT4G11790-mKOk
FEATURES             Location/Qualifiers
     source          1..12182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2029..2053)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3034..3052)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3035..3052)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3081..3090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3120..3139)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3121..3139)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3146..3163)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3146..3162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3155..3177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3320..3503
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3504..3545,3546..3554,3555..4325)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3585..3604)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4195..4214
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4950..5202
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5295..5319
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5840..6631
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6877..7465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7366..7385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7619..7636
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7651..7791
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7717..7736)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7877..7899
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8135..8329
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8395..9468)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9897..10526)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11857..11881
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12046..12067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12082..12087,12088..12105,12106..12112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12120..12136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12125..12147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12144..12160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12144..12160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gagaggagta aaacgcgccg
      721 ccatctccga atcgaatgac tctcccttta agaatgcaaa accagtggaa ggaatctttt
      781 ttggtactca gaagaatgtg gtaccgatgc agcagagttc agtaacatca gcatcattgg
      841 ataaacagag ggctgagtta gctaggaagc atgtgagagc tcttaataac caatttgtaa
      901 gttgggtgca gttacagctg aagaatcatc ctgatgaact ttgggaagat gggatgaatg
      961 actacattac gcatgcttca aacattctgg aaaagtttaa ggatgtggtc agctggctta
     1021 aagaaaataa gggaaagggg gagaatttat cccctgaatc tcgtggagca gaaaataaat
     1081 tagtggctga agtcaagaat accaatgtta aatcattttc aaacaatatt ccttttgctt
     1141 caaacaatca acctgggatc ttctcaaaca atcaatcttc cgatttttcc agtagtcagt
     1201 ctggtttctt ttcaagccaa tctggagcat tctccagcag tccttctggt ttgatatcca
     1261 atagccagac tggatctttt agcagtggcc agtttggtac gacaaaaagc agccaaccaa
     1321 gcctattttc cggcagtcaa gccggagcaa tctctaatag ccaacctgga actttctcca
     1381 gcagtcactc tggtataaca tctaatagcc agacgggatc ttttagtagt ggccaatttg
     1441 gtcagacaaa aagcagccaa ccaagcctac tttccggcag tcaagcagga gcaatctcta
     1501 gtaaccaacc ttcttttcaa ttttctaaca gtcaatcccc ttttacatct gcagtacctc
     1561 cagtgtccat accggcaaag caggatcact cagatgatgc ggatggtgga gacgaacaat
     1621 ctcaaccgag cagcccatct gtcaaaaaga cggaagaaaa gggtattact gtggttcatg
     1681 aagtcaaatg caaactttat gtcaagtcaa gtgacccagc agataaaggg tggaaagata
     1741 aaggaactgg gaatctctac ataaaatgca aagaaggagt cgacaagggg acaaaagaat
     1801 caaaacccac aattcttgtc cgaaacgatg ttggaaaact gcttctgaat gcactactgt
     1861 acgctggaat gaagacaagc ccacagaaga acgctcttgt tgcaatattt cactcctcgg
     1921 atgattccaa cgagaatgta acaccgagaa cctttctgat aaggacaaag aacgcagaag
     1981 ctagagataa tttagcaacg gccatccaag aatacgcccc ttcttcacac ccagctttct
     2041 tgtacaaagt ggtgatggga cgtccgcgga gatctacgcg tgtcgactcg agatatccaa
     2101 ctagtatggt gagcgttatc aagcctgaga tgaagatgag gtactacatg gacggaagcg
     2161 tgaacggaca cgagttcact attgaaggcg aaggtactgg aaggccttac gagggacatc
     2221 aagagatgac tctcagagtg accatggctg aaggtggacc tatgcctttc gctttcgatc
     2281 tcgtgtctca cgtgttctgc tacggacaca gagtgttcac taagtaccct gaagagatcc
     2341 cggactactt caagcaagct ttccctgagg gactctcttg ggagagatct ttggaattcg
     2401 aggacggtgg atccgcttct gtgtctgctc atatctctct caggggcaac accttctacc
     2461 acaagagcaa gtttaccggg gtgaacttcc ctgctgatgg accgatcatg cagaaccagt
     2521 ctgttgattg ggagccgtct actgagaaga tcaccgcttc tgatggtgtg ctcaagggtg
     2581 atgtgaccat gtaccttaag ctcgaaggtg gtggaaacca caagtgccag ttcaagacta
     2641 cctacaaggc cgccaaagaa atcctcgaga tgcctggtga tcactacatc ggacataggc
     2701 tcgtgagaaa gaccgaggga aacatcactg agcaggttga ggatgctgtg gctcattctc
     2761 aattgttata agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     2821 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     2881 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2941 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     3001 aggcatgcaa gctagcttac tagtgatgca tattctatag tgtcacctaa atctgcggcc
     3061 gcactagtga tatcccgcgg ccatggcggc cgggagcatg cgacgtcggg cccaattcgc
     3121 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3301 ggaaattgta agcgttaatg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     3361 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     3421 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     3481 actctcaatc caaataatct gcaatggcaa ttaccttatc cgcaacttct ttacctattt
     3541 ccgcccggat ccgggcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3601 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3661 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3721 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3781 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3841 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3901 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3961 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4021 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4081 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4141 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4201 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4261 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4321 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4381 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4441 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
     4501 tccaacactt acgtttgcaa cgtccaagag caaatagacc acgaacgccg gaaggttgcc
     4561 gcagcgtgtg gattgcgtct caattctctc ttgcaggaat gcaatgatga atatgatact
     4621 gactatgaaa ctttgaggga atactgccta gcaccgtcac ctcataacgt gcatcatgca
     4681 tgccctgaca acatggaaca tcgctatttt tctgaagaat tatgctcgtt ggaggatgtc
     4741 gcggcaattg cagctattgc caaaatcgaa atacccctca cgcatgcatt catcaatatt
     4801 attcatgcgg ggaaaggcaa gattaatcca actggcaaat catccagcgt gattggtaac
     4861 ttcagttcca gcgacttgat tcgttttggt gctacccacg ttttcaataa ggacgagatg
     4921 gtggagtaaa gaaggagtgc gtcgaagcag atcgttcaaa catttggcaa taaagtttct
     4981 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     5041 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     5101 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     5161 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     5221 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5281 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5341 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5401 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5461 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5521 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5581 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     5641 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5701 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5761 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5821 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5881 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5941 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     6001 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     6061 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     6121 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     6181 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     6241 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6301 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6361 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6421 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6481 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6541 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6601 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6661 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6721 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6781 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7021 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7561 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7621 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7681 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7741 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7801 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7861 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7921 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7981 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     8041 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     8101 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     8161 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     8221 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     8281 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8341 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8401 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8461 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8521 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8581 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     8641 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8701 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8761 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8821 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8881 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8941 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     9001 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     9061 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     9121 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     9181 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     9241 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9301 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9361 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9421 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     9481 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9541 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9601 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9661 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9721 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9781 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9841 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9901 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9961 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    10021 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    10081 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    10141 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    10201 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    10261 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    10321 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    10381 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    10441 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    10501 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10561 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10621 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10681 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10741 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10801 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10861 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10921 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10981 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    11041 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    11101 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    11161 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    11221 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    11281 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    11341 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    11401 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    11461 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    11521 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11581 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    11641 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11701 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    11761 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11821 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
    11881 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
    11941 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    12001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12061 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    12121 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
    12181 gg
//
