LOCUS       sequence-447330-       12209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G02100.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G02100-mKOk
FEATURES             Location/Qualifiers
     source          1..12209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2056..2080)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3061..3079)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3062..3079)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3108..3117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3147..3166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3148..3166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3173..3190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3173..3189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3182..3204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3347..3530
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3531..3572,3573..3581,3582..4352)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3612..3631)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4222..4241
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4977..5229
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5322..5346
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5867..6658
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6904..7492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7393..7412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7646..7663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7678..7818
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7744..7763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7904..7926
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8162..8356
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8422..9495)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9924..10553)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11884..11908
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12073..12094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12109..12114,12115..12132,12133..12139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12147..12163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12152..12174
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12171..12187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12171..12187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatggc ttctaacgat ccaaaaaacg
      721 gcggcggttt cttcgcctct cttgcttcat ccatcaccaa tttcggatcg gccatgagta
      781 aatcagtgaa cggtttgatg ggatatgaag ggcttgaagt gattaatcca gaaggaagta
      841 cagacgatgc agaggaagaa gcagggagag gaagatggaa gcaagaggaa cgtgatggct
      901 attggaagat gatgcagaag tatatagggt ctgatgttac atctatggtt actcttcctg
      961 tgatcatttt tgaacccatg acaatgttgc agaaaatggc tgagttgatg gaatactcat
     1021 atttgctaga catggctgac aaaactgaag atccttacat gcgtatggta tatgcatcat
     1081 catgggctat atctgtgtat tacgcctacc agcgtacctg gaagccgttc aatccaattc
     1141 tcggtgagac ttatgagatg actaatcata atgggatcaa ttttatagct gaacaggtct
     1201 gccatcaccc accaatgagt gctggtcatg ctgaaaacga gcatttcgct tacgattgca
     1261 cttcaaaact gaaaacgaaa ttcctaggca actcaattga tgtctaccca gtaggaagga
     1321 cacgagtgac acttaaaaga gatggtgtcg ttcttgatct tgtacctcca ctgacaaagg
     1381 ttcataacct tatcttcggt cgaacatggg tcgattcgcc aggagagatg gtcatgacga
     1441 acctgaccac tggtgacaaa gtggtgcttt actttcaacc atgtggctgg tttggatctg
     1501 gacgatatga agtggacggt tacgtgtata attcagctga agagcccaag atgctaatga
     1561 ccggaaaatg gaacgagtcc ttgagttatc agccatgtga tgcggaaggc gaacctcttc
     1621 caggcaccga actgaaggag gtgtggaagg ttgctgaagc tccaaagaac gataaatacc
     1681 aatacacaca ttttgctcac aagatcaata gttttgacac tgcccctaaa aagctactgt
     1741 catctgattc acgtctacgt cctgatagat acgcgctgga gatgggtgat atgtccaaat
     1801 ctggattcga aaagagcagc ctagaggata gacaaagagc tgagaagaaa agcagagaag
     1861 agaaaggcca aaaatttgct ccaaaatggt ttgatgaaac agaagaagtc actcctacac
     1921 catggggtga tctcgaagtt taccaattca atggaaagta ctcggtgcac cgcgccacag
     1981 ctgaaaactc tgaggataca accgatgtga agttgaccca attcaaccct tggcaattcc
     2041 aagatctctc tgctgaccca gctttcttgt acaaagtggt gatgggacgt ccgcggagat
     2101 ctacgcgtgt cgactcgaga tatccaacta gtatggtgag cgttatcaag cctgagatga
     2161 agatgaggta ctacatggac ggaagcgtga acggacacga gttcactatt gaaggcgaag
     2221 gtactggaag gccttacgag ggacatcaag agatgactct cagagtgacc atggctgaag
     2281 gtggacctat gcctttcgct ttcgatctcg tgtctcacgt gttctgctac ggacacagag
     2341 tgttcactaa gtaccctgaa gagatcccgg actacttcaa gcaagctttc cctgagggac
     2401 tctcttggga gagatctttg gaattcgagg acggtggatc cgcttctgtg tctgctcata
     2461 tctctctcag gggcaacacc ttctaccaca agagcaagtt taccggggtg aacttccctg
     2521 ctgatggacc gatcatgcag aaccagtctg ttgattggga gccgtctact gagaagatca
     2581 ccgcttctga tggtgtgctc aagggtgatg tgaccatgta ccttaagctc gaaggtggtg
     2641 gaaaccacaa gtgccagttc aagactacct acaaggccgc caaagaaatc ctcgagatgc
     2701 ctggtgatca ctacatcgga cataggctcg tgagaaagac cgagggaaac atcactgagc
     2761 aggttgagga tgctgtggct cattctcaat tgttataagc ggccatgcta gagtccgcaa
     2821 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     2881 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     2941 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     3001 tcctaaaacc aaaatccagt gacctgcagg catgcaagct agcttactag tgatgcatat
     3061 tctatagtgt cacctaaatc tgcggccgca ctagtgatat cccgcggcca tggcggccgg
     3121 gagcatgcga cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg
     3181 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3241 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3301 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatgggt ttctggagtt
     3361 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     3421 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     3481 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca atggcaatta
     3541 ccttatccgc aacttcttta cctatttccg cccggatccg ggcaggttct ccggccgctt
     3601 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3661 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3721 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3781 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3841 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3901 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3961 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4021 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4081 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4141 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4201 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4261 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4321 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4381 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4441 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4501 catgctggag ttcttcgccc accccgatcc aacacttacg tttgcaacgt ccaagagcaa
     4561 atagaccacg aacgccggaa ggttgccgca gcgtgtggat tgcgtctcaa ttctctcttg
     4621 caggaatgca atgatgaata tgatactgac tatgaaactt tgagggaata ctgcctagca
     4681 ccgtcacctc ataacgtgca tcatgcatgc cctgacaaca tggaacatcg ctatttttct
     4741 gaagaattat gctcgttgga ggatgtcgcg gcaattgcag ctattgccaa aatcgaaata
     4801 cccctcacgc atgcattcat caatattatt catgcgggga aaggcaagat taatccaact
     4861 ggcaaatcat ccagcgtgat tggtaacttc agttccagcg acttgattcg ttttggtgct
     4921 acccacgttt tcaataagga cgagatggtg gagtaaagaa ggagtgcgtc gaagcagatc
     4981 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     5041 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     5101 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     5161 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     5221 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa
     5281 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     5341 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     5401 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     5461 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     5521 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     5581 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     5641 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     5701 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     5761 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     5821 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     5881 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     5941 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     6001 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     6061 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     6121 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     6181 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     6241 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     6301 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     6361 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     6421 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     6481 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     6541 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     6601 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     6661 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     6721 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     6781 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     6841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7021 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     7681 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     7741 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     7801 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7861 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7921 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     7981 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     8041 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     8101 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     8161 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     8221 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     8281 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     8341 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     8401 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     8461 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     8521 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     8581 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     8641 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     8701 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     8761 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     8821 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     8881 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     8941 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     9001 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     9061 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     9121 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     9181 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     9241 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     9301 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     9361 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     9421 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     9481 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     9541 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     9601 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     9661 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     9721 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     9781 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     9841 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     9901 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     9961 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    10021 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    10081 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    10141 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    10201 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    10261 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    10321 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    10381 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    10441 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    10501 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    10561 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    10621 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    10681 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    10741 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    10801 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    10861 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    10921 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    10981 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    11041 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    11101 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    11161 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    11221 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    11281 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    11341 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    11401 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    11461 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    11521 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    11581 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    11641 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    11701 gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt taaagtactt
    11761 taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    11821 ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct
    11881 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac
    11941 gacaatctga tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
    12001 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    12061 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    12121 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    12181 ctatgacatg attacgaatt cgagctcgg
//
