LOCUS       sequence-447332-       11804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G65020.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G65020.1-mKOk
FEATURES             Location/Qualifiers
     source          1..11804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1651..1675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2656..2674)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2657..2674)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2703..2712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2742..2761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2743..2761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2768..2785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2768..2784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2777..2799)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2942..3125
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3126..3167,3168..3176,3177..3947)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3207..3226)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3817..3836
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4572..4824
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4917..4941
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5462..6253
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6499..7087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6988..7007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7241..7258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7273..7413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7339..7358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7499..7521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7757..7951
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8017..9090)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9519..10148)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11479..11503
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11668..11689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11704..11709,11710..11727,11728..11734)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11742..11758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11747..11769
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11766..11782
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11766..11782
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat ggcgtctctc aaagtcccaa
      721 gcaatgttcc tcttcccgaa gatgacgccg agcaactcca caaggctttt tcaggatggg
      781 gtaccaacga gaagctgatc atatcaatac tagctcacag gaacgcagca caacgcagct
      841 tgatccgcag cgtttatgca gctacctaca atgaggatct tctcaaagca ttagacaaag
      901 agctttctag cgactttgag agagctgtga tgttgtggac tcttgatcca ccagagagag
      961 atgcttattt ggctaaagaa tccaccaaga tgttcaccaa gaacaattgg gttcttgttg
     1021 aaatcgcttg cacaaggcct gctcttgagc ttatcaaggt caagcaagct taccaagctc
     1081 gatacaagaa atcaatcgag gaagatgtcg cgcaacacac atctggtgac cttcgtaagc
     1141 tcttgcttcc tcttgtgagc actttcaggt atgaaggaga tgatgtgaac atgatgcttg
     1201 caagatctga agctaagata cttcacgaga aggtctcaga gaaatcttac agtgacgatg
     1261 acttcatcag aatcttgaca acaagaagca aagcacagct cggtgcaaca ctcaaccact
     1321 acaacaacga gtatggaaac gccattaaca agaacttgaa ggaagagtcg gacgacaatg
     1381 actacatgaa actactaaga gctgtaatca catgtttgac ataccctgag aagcattttg
     1441 agaaggttct tcgtctatca atcaacaaaa tgggaacaga cgaatgggga ctaacccgag
     1501 tcgtgactac acgaactgaa gttgacatgg aacgcatcaa agaggaatat cagcgaagaa
     1561 acagcattcc tttggaccgt gctatcgcca aagacacttc tggtgactat gaggacatgc
     1621 ttgttgctct tctcggacat ggcgatgctc acccagcttt cttgtacaaa gtggtgatgg
     1681 gacgtccgcg gagatctacg cgtgtcgact cgagatatcc aactagtatg gtgagcgtta
     1741 tcaagcctga gatgaagatg aggtactaca tggacggaag cgtgaacgga cacgagttca
     1801 ctattgaagg cgaaggtact ggaaggcctt acgagggaca tcaagagatg actctcagag
     1861 tgaccatggc tgaaggtgga cctatgcctt tcgctttcga tctcgtgtct cacgtgttct
     1921 gctacggaca cagagtgttc actaagtacc ctgaagagat cccggactac ttcaagcaag
     1981 ctttccctga gggactctct tgggagagat ctttggaatt cgaggacggt ggatccgctt
     2041 ctgtgtctgc tcatatctct ctcaggggca acaccttcta ccacaagagc aagtttaccg
     2101 gggtgaactt ccctgctgat ggaccgatca tgcagaacca gtctgttgat tgggagccgt
     2161 ctactgagaa gatcaccgct tctgatggtg tgctcaaggg tgatgtgacc atgtacctta
     2221 agctcgaagg tggtggaaac cacaagtgcc agttcaagac tacctacaag gccgccaaag
     2281 aaatcctcga gatgcctggt gatcactaca tcggacatag gctcgtgaga aagaccgagg
     2341 gaaacatcac tgagcaggtt gaggatgctg tggctcattc tcaattgtta taagcggcca
     2401 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     2461 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     2521 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     2581 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc aagctagctt
     2641 actagtgatg catattctat agtgtcacct aaatctgcgg ccgcactagt gatatcccgc
     2701 ggccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt
     2761 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2821 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2881 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     2941 tgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     3001 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     3061 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     3121 ctgcaatggc aattacctta tccgcaactt ctttacctat ttccgcccgg atccgggcag
     3181 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3241 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3301 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3361 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3421 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3481 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3541 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3601 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3661 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3721 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3781 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3841 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3901 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3961 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4021 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4081 ccagcgcggg gatctcatgc tggagttctt cgcccacccc gatccaacac ttacgtttgc
     4141 aacgtccaag agcaaataga ccacgaacgc cggaaggttg ccgcagcgtg tggattgcgt
     4201 ctcaattctc tcttgcagga atgcaatgat gaatatgata ctgactatga aactttgagg
     4261 gaatactgcc tagcaccgtc acctcataac gtgcatcatg catgccctga caacatggaa
     4321 catcgctatt tttctgaaga attatgctcg ttggaggatg tcgcggcaat tgcagctatt
     4381 gccaaaatcg aaatacccct cacgcatgca ttcatcaata ttattcatgc ggggaaaggc
     4441 aagattaatc caactggcaa atcatccagc gtgattggta acttcagttc cagcgacttg
     4501 attcgttttg gtgctaccca cgttttcaat aaggacgaga tggtggagta aagaaggagt
     4561 gcgtcgaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4621 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4681 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4741 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4801 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     4861 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     4921 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     4981 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     5041 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     5101 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     5161 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     5221 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5281 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5341 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5401 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5461 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5521 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5581 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5641 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5701 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5761 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5821 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5881 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5941 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6001 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6061 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6121 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6181 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     6241 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     6301 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     6361 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     6421 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7261 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7321 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7381 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7441 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7501 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     7561 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     7621 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     7681 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     7741 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     7801 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     7861 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     7921 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     7981 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8041 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8101 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     8161 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     8221 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     8281 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     8341 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     8401 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     8461 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     8521 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     8581 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     8641 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     8701 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     8761 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     8821 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     8881 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     8941 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9001 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9061 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9121 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9181 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9241 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     9301 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     9361 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     9421 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     9481 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     9541 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     9601 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     9661 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     9721 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     9781 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     9841 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     9901 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     9961 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10021 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10081 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10141 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    10201 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    10261 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    10321 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    10381 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    10441 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    10501 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    10561 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    10621 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    10681 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    10741 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    10801 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    10861 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    10921 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    10981 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11041 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11101 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    11161 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    11221 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    11281 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
    11341 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    11401 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    11461 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    11521 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagccaa tacgcaaacc
    11581 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    11641 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    11701 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    11761 tcacacagga aacagctatg acatgattac gaattcgagc tcgg
//
