LOCUS       sequence-447333-       11237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT4G00165.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT4G00165-mKOk
FEATURES             Location/Qualifiers
     source          1..11237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1084..1108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2089..2107)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2090..2107)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2136..2145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2175..2194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2176..2194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2201..2218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2201..2217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2210..2232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2375..2558
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2559..2600,2601..2609,2610..3380)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2640..2659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3250..3269
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4005..4257
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4350..4374
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4895..5686
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5932..6520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6421..6440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6674..6691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6706..6846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6772..6791)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6932..6954
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7190..7384
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7450..8523)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8952..9581)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10912..10936
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11101..11122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11137..11142,11143..11160,11161..11167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11175..11191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11180..11202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11199..11215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11199..11215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gggtatctcg aaagctttgc
      721 gatctcttct catcttgctg ctccttaaca taacattctt cttcggccat gttacaccag
      781 gagccaccgt caagccatgc cctccccctc ccgcaaaaca agccaccacg aaatgtccaa
      841 gagacacact caaatttgga gtgtgtggga gctggttagg tctggttagt gaggtcatcg
      901 gtacaccacc gagccaggag tgttgctcgc tcatcaaagg tttggctgac tttgaagctg
      961 ccgtttgtct gtgcactgca cttaaaacca gcattctcgg agttgctcct gtcaaaattc
     1021 ccgttgctct cactctgctt ctcaactctt gcggcaaaaa tgttcctcag ggattcgtct
     1081 gccacccagc tttcttgtac aaagtggtga tgggacgtcc gcggagatct acgcgtgtcg
     1141 actcgagata tccaactagt atggtgagcg ttatcaagcc tgagatgaag atgaggtact
     1201 acatggacgg aagcgtgaac ggacacgagt tcactattga aggcgaaggt actggaaggc
     1261 cttacgaggg acatcaagag atgactctca gagtgaccat ggctgaaggt ggacctatgc
     1321 ctttcgcttt cgatctcgtg tctcacgtgt tctgctacgg acacagagtg ttcactaagt
     1381 accctgaaga gatcccggac tacttcaagc aagctttccc tgagggactc tcttgggaga
     1441 gatctttgga attcgaggac ggtggatccg cttctgtgtc tgctcatatc tctctcaggg
     1501 gcaacacctt ctaccacaag agcaagttta ccggggtgaa cttccctgct gatggaccga
     1561 tcatgcagaa ccagtctgtt gattgggagc cgtctactga gaagatcacc gcttctgatg
     1621 gtgtgctcaa gggtgatgtg accatgtacc ttaagctcga aggtggtgga aaccacaagt
     1681 gccagttcaa gactacctac aaggccgcca aagaaatcct cgagatgcct ggtgatcact
     1741 acatcggaca taggctcgtg agaaagaccg agggaaacat cactgagcag gttgaggatg
     1801 ctgtggctca ttctcaattg ttataagcgg ccatgctaga gtccgcaaaa atcaccagtc
     1861 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     1921 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     1981 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     2041 aatccagtga cctgcaggca tgcaagctag cttactagtg atgcatattc tatagtgtca
     2101 cctaaatctg cggccgcact agtgatatcc cgcggccatg gcggccggga gcatgcgacg
     2161 tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     2221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2341 gcctgaatgg cgaatggaaa ttgtaagcgt taatgggttt ctggagttta atgagctaag
     2401 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     2461 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     2521 tagagtctca tattcactct caatccaaat aatctgcaat ggcaattacc ttatccgcaa
     2581 cttctttacc tatttccgcc cggatccggg caggttctcc ggccgcttgg gtggagaggc
     2641 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2701 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     2761 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     2821 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     2881 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2941 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3001 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     3061 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     3121 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     3181 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     3241 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     3301 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     3361 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     3421 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3481 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3541 cttcgcccac cccgatccaa cacttacgtt tgcaacgtcc aagagcaaat agaccacgaa
     3601 cgccggaagg ttgccgcagc gtgtggattg cgtctcaatt ctctcttgca ggaatgcaat
     3661 gatgaatatg atactgacta tgaaactttg agggaatact gcctagcacc gtcacctcat
     3721 aacgtgcatc atgcatgccc tgacaacatg gaacatcgct atttttctga agaattatgc
     3781 tcgttggagg atgtcgcggc aattgcagct attgccaaaa tcgaaatacc cctcacgcat
     3841 gcattcatca atattattca tgcggggaaa ggcaagatta atccaactgg caaatcatcc
     3901 agcgtgattg gtaacttcag ttccagcgac ttgattcgtt ttggtgctac ccacgttttc
     3961 aataaggacg agatggtgga gtaaagaagg agtgcgtcga agcagatcgt tcaaacattt
     4021 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     4081 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     4141 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     4201 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac
     4261 cggcatgcaa gctgataatt caattcggcg ttaattcagt acattaaaaa cgtccgcaat
     4321 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     4381 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     4441 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     4501 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     4561 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc
     4621 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4681 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4741 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4801 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4861 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     4921 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4981 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     5041 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     5101 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     5161 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     5221 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     5281 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     5341 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     5401 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5461 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5521 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5581 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5641 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     5701 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc
     5761 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     5821 ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga
     5881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6061 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     6721 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     6781 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     6841 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6961 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     7021 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     7081 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     7141 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     7201 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     7261 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     7321 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     7381 cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     7441 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     7501 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     7561 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     7621 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     7681 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     7741 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     7801 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     7861 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     7921 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     7981 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     8041 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     8101 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     8161 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     8221 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     8281 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
     8341 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     8401 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     8461 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     8521 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     8581 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     8641 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     8701 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     8761 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     8821 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     8881 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     8941 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     9001 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     9061 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     9121 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     9181 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     9241 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     9301 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     9361 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     9421 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     9481 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     9541 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     9601 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     9661 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     9721 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
     9781 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
     9841 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
     9901 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
     9961 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
    10021 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
    10081 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
    10141 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
    10201 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
    10261 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
    10321 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
    10381 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
    10441 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
    10501 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    10561 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    10621 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    10681 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    10741 gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta aagtactttg
    10801 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    10861 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
    10921 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
    10981 caagctcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11041 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11101 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11161 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    11221 tacgaattcg agctcgg
//
