LOCUS       sequence-447335-       12221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G59420.1 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G59420-mKOk
FEATURES             Location/Qualifiers
     source          1..12221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2068..2092)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3073..3091)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3074..3091)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3120..3129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3159..3178)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3160..3178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3185..3202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3185..3201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3194..3216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        3359..3542
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3543..3584,3585..3593,3594..4364)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3624..3643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4234..4253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4989..5241
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5334..5358
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5879..6670
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6916..7504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7405..7424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7658..7675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7690..7830
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7756..7775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7916..7938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8174..8368
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8434..9507)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9936..10565)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11896..11920
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12085..12106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12121..12126,12127..12144,12145..12151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12159..12175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12164..12186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12183..12199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12183..12199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcatggg aagtccaaag aagaacgaga
      721 acaaaggctt cttcgccgcc atgacttctg gcttctccat gttcggtacc gccgtgtcga
      781 gatccgttaa cggcgtgcaa ggtaatgaag gagttgaggt cataaatcca gaaggtggca
      841 aggaagatgc tgaagaggaa gctcagaaag gaaggtggaa ggacgaggaa cgagatagtt
      901 actggaagat gatgcagaaa tatataggtt cggatattac gtcaatggtg actcttcctg
      961 ttgttatatt tgagcctatg actatgctcc agaagatggc tgagataatg gagtattctc
     1021 atttgttgga tcaagcagat gaatgcgaag atccatactt gcgtttagta tatgcttcat
     1081 catgggctat atctgtttac tatgccttcc aacgaacttg gaagcctttc aatcctattc
     1141 ttggggagac atatgagatg gtcaaccatg gtgggatttc ttttatttct gagcaggtta
     1201 gccatcatcc accaatgagt gctggtcatg ccgagaacga gcacttcatt tacgacatca
     1261 catcaaagtt gaaaactaaa cttttgggta actctgttga tgtttaccct gtgggaagaa
     1321 cgcgtgtaac cctcaagaaa gatggtgtgg ttctggattt ggtgccgcct ctcactaaga
     1381 ttcacaatct aatatttgga cgaacctggg ttgactcacc tggggaaatg gtcatgacaa
     1441 atttaaccac tggagacaaa gttgtgcttt atttccagcc atgtggctgg ttcggttctg
     1501 gccgctatga agttgatggc tacgtttaca gcgcagctga agaaccgaaa atcatgatga
     1561 caggaaaatg gaatgagaaa atgagctacc aaccttgtga tgccgaaggg gaaccccttc
     1621 caggaacaga gctgaaagag gtgtggcatt tggctgatgt ccccaaaaac gacaactttc
     1681 agtacactca ctttgctcac aagataaaca gcttcgacac agcgcctgct aagctcttgg
     1741 cttcagactc acgtatccgt cctgatagat attcccttga gcagggtgac ctttctaaag
     1801 ctggttccga gaaacacagc cttgaggaga gacaaagggc cgaaaagagg accagagaga
     1861 caaagggaca aaagttcact ccaagatggt tcgatctaac ggatgagatc acacctactc
     1921 catggggaga tattgaagta taccaataca acgggaagta caatgaacac cgagacacgg
     1981 cagagagctc aagtagtgcc tccaacgaaa cggacctcaa atcgatcgag tttaatcctt
     2041 ggcaatatgg taatatctca accgaagacc cagctttctt gtacaaagtg gtgatgggac
     2101 gtccgcggag atctacgcgt gtcgactcga gatatccaac tagtatggtg agcgttatca
     2161 agcctgagat gaagatgagg tactacatgg acggaagcgt gaacggacac gagttcacta
     2221 ttgaaggcga aggtactgga aggccttacg agggacatca agagatgact ctcagagtga
     2281 ccatggctga aggtggacct atgcctttcg ctttcgatct cgtgtctcac gtgttctgct
     2341 acggacacag agtgttcact aagtaccctg aagagatccc ggactacttc aagcaagctt
     2401 tccctgaggg actctcttgg gagagatctt tggaattcga ggacggtgga tccgcttctg
     2461 tgtctgctca tatctctctc aggggcaaca ccttctacca caagagcaag tttaccgggg
     2521 tgaacttccc tgctgatgga ccgatcatgc agaaccagtc tgttgattgg gagccgtcta
     2581 ctgagaagat caccgcttct gatggtgtgc tcaagggtga tgtgaccatg taccttaagc
     2641 tcgaaggtgg tggaaaccac aagtgccagt tcaagactac ctacaaggcc gccaaagaaa
     2701 tcctcgagat gcctggtgat cactacatcg gacataggct cgtgagaaag accgagggaa
     2761 acatcactga gcaggttgag gatgctgtgg ctcattctca attgttataa gcggccatgc
     2821 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     2881 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     2941 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3001 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag ctagcttact
     3061 agtgatgcat attctatagt gtcacctaaa tctgcggccg cactagtgat atcccgcggc
     3121 catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca
     3181 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3241 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3301 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatgg
     3361 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     3421 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     3481 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg
     3541 caatggcaat taccttatcc gcaacttctt tacctatttc cgcccggatc cgggcaggtt
     3601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3721 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4081 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     4321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     4381 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4441 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4501 gcgcggggat ctcatgctgg agttcttcgc ccaccccgat ccaacactta cgtttgcaac
     4561 gtccaagagc aaatagacca cgaacgccgg aaggttgccg cagcgtgtgg attgcgtctc
     4621 aattctctct tgcaggaatg caatgatgaa tatgatactg actatgaaac tttgagggaa
     4681 tactgcctag caccgtcacc tcataacgtg catcatgcat gccctgacaa catggaacat
     4741 cgctattttt ctgaagaatt atgctcgttg gaggatgtcg cggcaattgc agctattgcc
     4801 aaaatcgaaa tacccctcac gcatgcattc atcaatatta ttcatgcggg gaaaggcaag
     4861 attaatccaa ctggcaaatc atccagcgtg attggtaact tcagttccag cgacttgatt
     4921 cgttttggtg ctacccacgt tttcaataag gacgagatgg tggagtaaag aaggagtgcg
     4981 tcgaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     5041 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     5101 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     5161 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     5221 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     5281 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     5341 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     5401 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     5461 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     5521 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     5581 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     5641 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     5701 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     5761 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     5821 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     5881 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     5941 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     6001 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     6061 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     6121 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     6181 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     6241 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     6301 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     6361 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     6421 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     6481 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     6541 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     6601 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     6661 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     6721 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     6781 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     6841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7021 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7681 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     7801 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     7861 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7921 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     7981 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     8041 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     8101 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     8161 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     8221 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     8281 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     8341 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     8401 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8461 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8521 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8581 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     8641 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     8701 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8761 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     8821 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     8881 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     8941 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     9001 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     9061 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     9121 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     9181 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     9241 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     9301 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     9361 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9421 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9481 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     9541 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9601 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     9661 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     9721 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     9781 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     9841 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     9901 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     9961 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    10021 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    10081 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    10141 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    10201 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    10261 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    10321 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    10381 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10441 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10501 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10561 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10621 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    10681 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    10741 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    10801 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    10861 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    10921 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    10981 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    11041 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    11101 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    11161 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    11221 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    11281 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    11341 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    11401 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11461 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11521 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11581 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    11641 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    11701 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
    11761 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    11821 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
    11881 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
    11941 aaggcgggaa acgacaatct gatccaagct caagctgctc tagccaatac gcaaaccgcc
    12001 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    12061 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12121 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12181 cacaggaaac agctatgaca tgattacgaa ttcgagctcg g
//
