LOCUS       sequence-447338-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of AT5G65020.2 coding sequence tagged with 
            mKOk in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis 
            A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL
  TITLE     Lipid Metabolism and Contact Site Homologs Are Enriched at the 
            Chloroplast Envelope-Thylakoid Interface
  JOURNAL   bioRxiv 2025.06.20.660776; doi: 
            https://doi.org/10.1101/2025.06.20.660776
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776"
COMMENT     Sequence Label: pUBC(k)-AT5G65020.2-mKOk
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1606..1630)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2611..2629)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2612..2629)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2658..2667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2697..2716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2698..2716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2723..2740)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2723..2739)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2754)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        2897..3080
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(3081..3122,3123..3131,3132..3902)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3162..3181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3772..3791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4527..4779
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4872..4896
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5417..6208
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6454..7042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6943..6962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7196..7213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7228..7368
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7294..7313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7454..7476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7712..7906
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7972..9045)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9474..10103)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11434..11458
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11623..11644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11659..11664,11665..11682,11683..11689)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11697..11713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11702..11724
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11721..11737
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11721..11737
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tacccgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt
       61 gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa
      121 aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt
      181 caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa
      241 aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa
      301 aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct
      361 caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat
      421 tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt
      481 ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat
      541 aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat
      601 cgaatttgta gattaatctg agtttttctg attaacactc gagtgcggga tcctctagag
      661 ggcccatcac aagtttgtac aaaaaagcag gcttcaccat gtttgttttt tgggtttggt
      721 ctgaaacagg atggggtacc aacgagaagc tgatcatatc aatactagct cacaggaacg
      781 cagcacaacg cagcttgatc cgcagcgttt atgcagctac ctacaatgag gatcttctca
      841 aagcattaga caaagagctt tctagcgact ttgagagagc tgtgatgttg tggactcttg
      901 atccaccaga gagagatgct tatttggcta aagaatccac caagatgttc accaagaaca
      961 attgggttct tgttgaaatc gcttgcacaa ggcctgctct tgagcttatc aaggtcaagc
     1021 aagcttacca agctcgatac aagaaatcaa tcgaggaaga tgtcgcgcaa cacacatctg
     1081 gtgaccttcg taagctcttg cttcctcttg tgagcacttt caggtatgaa ggagatgatg
     1141 tgaacatgat gcttgcaaga tctgaagcta agatacttca cgagaaggtc tcagagaaat
     1201 cttacagtga cgatgacttc atcagaatct tgacaacaag aagcaaagca cagctcggtg
     1261 caacactcaa ccactacaac aacgagtatg gaaacgccat taacaagaac ttgaaggaag
     1321 agtcggacga caatgactac atgaaactac taagagctgt aatcacatgt ttgacatacc
     1381 ctgagaagca ttttgagaag gttcttcgtc tatcaatcaa caaaatggga acagacgaat
     1441 ggggactaac ccgagtcgtg actacacgaa ctgaagttga catggaacgc atcaaagagg
     1501 aatatcagcg aagaaacagc attcctttgg accgtgctat cgccaaagac acttctggtg
     1561 actatgagga catgcttgtt gctcttctcg gacatggcga tgctcaccca gctttcttgt
     1621 acaaagtggt gatgggacgt ccgcggagat ctacgcgtgt cgactcgaga tatccaacta
     1681 gtatggtgag cgttatcaag cctgagatga agatgaggta ctacatggac ggaagcgtga
     1741 acggacacga gttcactatt gaaggcgaag gtactggaag gccttacgag ggacatcaag
     1801 agatgactct cagagtgacc atggctgaag gtggacctat gcctttcgct ttcgatctcg
     1861 tgtctcacgt gttctgctac ggacacagag tgttcactaa gtaccctgaa gagatcccgg
     1921 actacttcaa gcaagctttc cctgagggac tctcttggga gagatctttg gaattcgagg
     1981 acggtggatc cgcttctgtg tctgctcata tctctctcag gggcaacacc ttctaccaca
     2041 agagcaagtt taccggggtg aacttccctg ctgatggacc gatcatgcag aaccagtctg
     2101 ttgattggga gccgtctact gagaagatca ccgcttctga tggtgtgctc aagggtgatg
     2161 tgaccatgta ccttaagctc gaaggtggtg gaaaccacaa gtgccagttc aagactacct
     2221 acaaggccgc caaagaaatc ctcgagatgc ctggtgatca ctacatcgga cataggctcg
     2281 tgagaaagac cgagggaaac atcactgagc aggttgagga tgctgtggct cattctcaat
     2341 tgttataagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     2401 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     2461 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     2521 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg
     2581 catgcaagct agcttactag tgatgcatat tctatagtgt cacctaaatc tgcggccgca
     2641 ctagtgatat cccgcggcca tggcggccgg gagcatgcga cgtcgggccc aattcgccct
     2701 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2761 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2821 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     2881 aattgtaagc gttaatgggt ttctggagtt taatgagcta agcacatacg tcagaaacca
     2941 ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa
     3001 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
     3061 ctcaatccaa ataatctgca atggcaatta ccttatccgc aacttcttta cctatttccg
     3121 cccggatccg ggcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3181 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3241 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3301 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3361 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3421 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3481 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3541 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3601 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3661 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3721 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3901 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     3961 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4021 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccgatcc
     4081 aacacttacg tttgcaacgt ccaagagcaa atagaccacg aacgccggaa ggttgccgca
     4141 gcgtgtggat tgcgtctcaa ttctctcttg caggaatgca atgatgaata tgatactgac
     4201 tatgaaactt tgagggaata ctgcctagca ccgtcacctc ataacgtgca tcatgcatgc
     4261 cctgacaaca tggaacatcg ctatttttct gaagaattat gctcgttgga ggatgtcgcg
     4321 gcaattgcag ctattgccaa aatcgaaata cccctcacgc atgcattcat caatattatt
     4381 catgcgggga aaggcaagat taatccaact ggcaaatcat ccagcgtgat tggtaacttc
     4441 agttccagcg acttgattcg ttttggtgct acccacgttt tcaataagga cgagatggtg
     4501 gagtaaagaa ggagtgcgtc gaagcagatc gttcaaacat ttggcaataa agtttcttaa
     4561 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     4621 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4681 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4741 ataaattatc gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa
     4801 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     4861 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     4921 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     4981 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     5041 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     5101 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     5161 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     5221 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     5281 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5341 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5401 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5461 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5521 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     5581 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     5641 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     5701 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     5761 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     5821 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     5881 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     5941 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6001 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6061 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6121 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6181 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     6241 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     6301 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     6361 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6601 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7201 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7261 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7321 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     7381 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7441 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7501 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     7561 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     7621 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     7681 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     7741 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     7801 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     7861 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     7921 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     7981 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     8041 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     8101 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8161 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     8221 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     8281 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     8341 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     8401 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     8461 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     8521 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     8581 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     8641 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     8701 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     8761 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     8821 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     8881 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     8941 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     9001 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     9061 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9121 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9181 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     9241 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     9301 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     9361 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     9421 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     9481 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     9541 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     9601 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     9661 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     9721 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     9781 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     9841 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     9901 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     9961 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10021 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10081 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10141 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10201 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    10261 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    10321 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    10381 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    10441 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    10501 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    10561 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    10621 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    10681 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    10741 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    10801 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    10861 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    10921 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    10981 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    11041 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    11101 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    11161 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    11221 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    11281 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
    11341 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    11401 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
    11461 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta
    11521 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    11581 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    11641 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    11701 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcgg
//
