LOCUS       sequence-44735-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS
  TITLE     Golgi maturation visualized in living yeast.
  JOURNAL   Nature. 2006 Jun 22. 441(7096):1002-6.
  PUBMED    16699524
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 22. 441(7096):1002-6."
COMMENT     Sequence Label: pSSec7-mGFPx3
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      579..588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(630..651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgcaatgtgt tttacagctt ttgatgatag agctgctcaa cgaattattc gagaacgaag
       61 attttgccca ctgtatccct tacaaggaag ccattagaat tacaagattg ttggagaaat
      121 cgtacgaatt ttctcgtgac tttaatgaag attatgggtt aagaacaaga ctagtagagg
      181 ctcgtgtagt tgataaaata ccaaacctgt tgaaacaaga aacgagtgct gctgcagttc
      241 tccttgacat tatgtttcag ttgtatctga acgatgatga gaagaaggct gatttaataa
      301 ctcgtctgat caccatttgc atccaggtcg tagagggtta tgtttctttg gatgatagaa
      361 ccatggaacg tagtatcaat gcctggcgtt cagtgatagt tgaaatatta caaggttact
      421 acgaatttga tgatgaagac tttagactat actgtcctgc tatgtatgct ctggtgattc
      481 aaatattaga taagagcgtt ccaacggagt tgagacatgc tataaaacaa tttctaagca
      541 gagttggtga attatacctt tctacggatc caccggtcgc caccatggtg agcaagggcg
      601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat g
//
