LOCUS       sequence-447358-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses two precursor peptides and the lanthipeptide synthetase 
            from LanII-57 (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanII-57
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3989..4006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4293..4340
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4345..4363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4364..4388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      4440..4449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      7769..7816
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7857..8312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7944..7963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8154..8175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8404..9219)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8404..9219)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9127..9146
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9211..9230
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgtcggataa tagtacagct cgtgcatgga
     3601 aagacccaga agatgcagca cgcgcagtcg caccggcgca tccgagcggt gaggttgatc
     3661 ttggattatt tgttggcggt ttgtcagatg aagaaggcgg cagcgaagca ttgggtacag
     3721 gcggctgttg tccgacctgg gcaacaggta cccattgtta actagaagcc cggcagagtt
     3781 agttcgaacc taatgccaca aatgagacag gacgccaatg ggtaccggac attaggtcga
     3841 gctcagttcg gtaacggaga gaccctgcgg cgcggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttgccctta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatgca tcaccaccac caccatgcca tgtcgaatga
     4021 agatatcgtt cgctcctgga aagatccgga gacggctcgt accgctccgg gccacccagt
     4081 gggtgaaccg gacctgaccg ctctgtccgg tggtgaggaa cagattaccg tatatgatat
     4141 ttgtacgacg aatcagaccg gatgtgggac ttgcggtagc ttcagttttg gctgttgttg
     4201 actagaagcc cggcagagtt agttcgaacc taatgccaca aatgagacag gacgccaatg
     4261 ggtaccggac attaggtcga gctcagttcg gtctagcata accccttggg gcctctaaac
     4321 gggtcttgag gggttttttg gctctaatac gactcactat aggggaattg tgagcggata
     4381 acaattccaa ggagatatac aatgccgtca aatactccac cggatgccgc agccgcagcg
     4441 ccaccatggt cacgtgtgaa tcgtagtcct ttgacgggtt catggtgggc acgtggcctg
     4501 tggctggcgg aacgcttatc aggtgaggga gatccgcctg caccagtagc agataatcca
     4561 gcggaaggtt cattaggatg gcgtgcggat cgtcgtctgg cacgttggcg ttcggccttt
     4621 accgatcatg cccctgatac agccgaagca caagcccgtg cactcgggat tgatccacct
     4681 ctgcttcgcc gtttgcttgc agaacatcct gattcattag ccgctcgtac agcgaaaccg
     4741 gcttgggcac gcttcgttga agatgttgtt gcacgtgctc ctcgtctgcc gccggcccct
     4801 gatggtgaag atactacaga acatgatgaa gaacatgatg gaacacatcg tcatggtttt
     4861 gctgactttg cggcagttgt agaacctttt ctggctgaag cattacgttt acttgaagcg
     4921 cccgcccgta ccgctgatgc aacggaaccc agtgcagccg ttcttgcagc ggtgcgccgt
     4981 tcgctgaccg cgggtctgcg tgatcaatta gttagtgtac ttggtcgcac cttagtgtta
     5041 gaacttcatg ttgctcgttt gtctggtcag ttaagtggtg atacaccaca ggaacgtttc
     5101 catgcattta ttgcgtggca acgtgaaccg gaacgcttag cggcactgct tgatgagtat
     5161 gctgttctgg cgcgtttatt ggcatgtgcg acccgtaatg cggtacatgc gcatcgtgaa
     5221 tttctggatc gttttactgc agatcgtcct gcactcgttg aacgtctctt tgctggacgt
     5281 gatcctgggg agctgactga agtccgtaca ggtcgcggtg acggtcatga agctggacgt
     5341 tcagttgcat tgctgacatt ttcatctggg gcgcgtcttg tttataaacc tcgtccactt
     5401 gcagtacatg aacattttaa tgcgttagta gattggtatg acactgtatt acctgttccg
     5461 ggtccgggta ccgtagcagt gtgggatcgt ggtggttatg gatggattga atatgttgaa
     5521 cccgcacctt gtgccgatca tgcagccgca gaacgtttct accatcgtct cggtgcactg
     5581 ctcgcattgt tatatgccac cgcagcaacc gattttcatt ttgaaaatgt gattgccgtg
     5641 ggtgaacatc ctgtggccgt agatttggaa tctttgttcc atgtgggtgt tccgcatctc
     5701 accggttcac cactgattga tgatgacccc gctcagcacc tgcttgcgca ttctgttcaa
     5761 cgtgtgggtc tgctcccgtc ggtgcttgta ggcgatggcg gcgcagtcct ggatgtaggt
     5821 ggtatgggtg ctgacaaagg agcagtgatg ccttttaaaa gcccaacatg ggaaggagat
     5881 ggtacagatg aaatgcgttt gacacgtgtt caacgtgcat ttgaaggaag cgcaaatcgt
     5941 cctcgtttag atgggcgtga tgtagatccg gcggatcatc tggcgtcctt acgtgccggc
     6001 ttcgaagcgg gttatcgtgc gattgcggca caccgtgaag cccttcttgc tcctgatggc
     6061 cccctggaac gctttcgcga agatacttgt cgtaccattg tacgcgctac gcgcgtttat
     6121 gcccatcttc tgggcgaatc gactcatccg gatgtaactc gcgatgcatt ggatcgtgat
     6181 cgcgtattag gattaatttt cgcacaaagt gcagggaacc cggcgcaacg tccattgctc
     6241 cgtttagaag ccgaagattt atggtcaggt gatgtcccgt tatttcgtag cgttgtgggt
     6301 tcacgtgatc ttcgtacaca tcgtggccgt agtttgccgc gtgcactccc agaaagcggg
     6361 ttggatcgtg cacgtcgcat tgtagctgcc atggatgaac atgatttagc tactcaggat
     6421 tggattattc gtgcagcttt tgcggcacgt gcaaccactg gtggtgcaga tggtgcggca
     6481 ccagcaggtt caggtccacg tcctgccggc cctgcaactg gtccggaccg tagtcccgga
     6541 gaacgcgcac tgcgtttggc gcgttccgtt gcggaccgct tattggccga tgcgcgccgc
     6601 gatggtggtc gcgcgggttg gctcggtctg gatttaagtc gcgatcaatg gcgtgtaaca
     6661 gcactgcgtg atgatttata tggtggttat cctggtacag ctctgttctt cgcacaactt
     6721 gcagcggtca caggtgaaga acgttatgca cgctgggcac gccgtattct ggcacctttt
     6781 ccggaacatt tagctcacgt attacgtcat acgccgaacc gctcctgggg tgcatttggt
     6841 ggcctggcag gtacggccta tgctttagac tgcgccgctg tactgctgga cgatcctgcg
     6901 ctggcggaac cggtaccagg tttattcact gccgctgctg gagctttaga tgatgatcgt
     6961 cagttagact taattggtgg cgcagcgggc tgtttggcgg ttgccgaagc gcttgcgcat
     7021 cgttatggtg ctgctgcgca tgattgtgct cgccgctgtg cacgtttatt ggttgagaaa
     7081 gcggaagctc atccgggtgg tactgtagca tggcgtacgg ctatgccagc ggcacgtcct
     7141 ttactgggta tgtcgcatgg cgcgggtggt attggttggg ctctgttacg tcatgcagct
     7201 cgtaccggag atccagccgc gctgcgcacg ggtcaagcgg ctttagcata cgaagaagcg
     7261 ggttatgatc cagaagtgga caactggcct gattaccgtg aacaggctcc ccgtccatgg
     7321 cgtgcgacac caccacccgg tacatttgca gccgtacatg cgtggtgtca tggtgcacct
     7381 ggcattggtc tgcttcgcgc agatttacct gcgggagttc gtactccatc cagcgatcgc
     7441 gctctcctgc gtgctgtccg ctcgaccgcc gatgcgggcc aaattggtga tgatagcctg
     7501 tgtcatggta ctctgggtaa tttagaattg tttgctgctg cagcgcgtgc aggcgtagcg
     7561 cgtgctcgtg atgatcacag ccgtacgcgt gatgcagtcc tggccgctat tgaagagcgt
     7621 ggaccggttt gtggaacgcc tggtggcatt gtgactcctg gtctgatgac aggcttagca
     7681 ggcattggtc atggtttgct gcgtattgca gcccctgaac gtgtggcacc tgtcctcttg
     7741 ctggccccgc aagaagcccc gtaacggtct agcataaccc cttggggcct ctaaacgggt
     7801 cttgaggggt tttttgagcg ctgaaaggag gaactatatc cggattggcg aatgggacgc
     7861 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7921 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7981 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8041 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8101 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8161 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8221 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8281 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     8401 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     8461 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     8521 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     8581 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     8641 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     8701 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     8761 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     8821 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     8881 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     8941 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     9001 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     9061 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     9121 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     9181 cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     9241 tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9301 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
