LOCUS       sequence-447377-        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC
  TITLE     Characterization of BioID tagging systems in budding yeast and 
            exploring the interactome of the Ccr4-Not complex.
  JOURNAL   G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221.
  PUBMED    39271111
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221."
COMMENT     Sequence Label: pRS416-ADH1p-BirA-FKBP-NLS-HA3
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..354,355..357,358..966)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MSKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLR
                     KLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQ
                     GWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLII
                     GDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             985..1305
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1312..1332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1333..1422
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atgtccaagg acaacaccgt gcccctgaag ctgatcgccc tgctggccaa cggcgagttc
       61 cactctggcg agcagctggg agagaccctg ggaatgagca gagccgccat caacaagcac
      121 atccagacac tgagagactg gggagtggac gtgttcaccg tgcctggcaa gggctacagc
      181 ctgcctgagc ctatccagct gctgaacgcc aagcagatcc tgggacagct ggatggcgga
      241 agcgtggccg tgctgcctgt gatcgactcc accaatcagt acctgctgga cagaatcgga
      301 gagctgaagt ccggcgacgc ctgcatcgcc gagtaccagc aggctggcag aggaggcaga
      361 ggacggaagt ggttcagccc attcggagcc aacctgtacc tgtccatgtt ctggagactg
      421 gagcagggac ctgctgctgc catcggactg agtctggtga tcggaatcgt gatggccgag
      481 gtgctgagaa agctgggagc cgacaaggtg agagtgaagt ggcctaatga cctgtacctc
      541 caggaccgca agctggctgg catcctggtg gagctgacag gcaagacagg cgatgccgct
      601 cagatcgtga tcggagccgg aatcaacatg gccatgagaa gagtggagga gagcgtggtg
      661 aaccagggct ggatcaccct gcaggaggct ggcatcaacc tggaccggaa caccctggcc
      721 gccatgctga tcagagagct gagagccgct ctggagctgt tcgagcagga gggactggct
      781 ccttacctga gcagatggga gaagctggac aacttcatca acagacctgt gaagctgatc
      841 atcggcgaca aggaaatctt cggcatctcc agaggaatcg acaagcaggg agctctgctg
      901 ctggagcagg acggaatcat caagccctgg atgggcggag aaatctccct gagaagcgca
      961 gagaagggtt ctggtggtgc tagcggagtg caggtggaaa ccatctcccc aggagacggg
     1021 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
     1081 ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     1141 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     1201 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     1261 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactcc accaaaaaag
     1321 aaaagaaaag tttacccata cgatgttcct gactatgcgg gctatccgta tgacgtcccg
     1381 gactatgcag gatcttatcc atatgacgtt ccagattacg ctgcttga
//
