LOCUS       sequence-447379-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC
  TITLE     Characterization of BioID tagging systems in budding yeast and 
            exploring the interactome of the Ccr4-Not complex.
  JOURNAL   G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221.
  PUBMED    39271111
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221."
COMMENT     Sequence Label: pRS416-ADH1p-BioID2-FRB-NLS-HA3
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="MSFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRK
                     WLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKK
                     VSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRIS
                     ENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIK
                     EILSGEFSLRRS"
     CDS             721..999
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             1009..1029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1030..1119
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atgtccttca agaacctgat ctggctgaag gaggtggaca gcacccagga gagactgaag
       61 gagtggaacg tgagctacgg caccgccctg gtggccgaca gacagaccaa gggcagaggc
      121 ggcctgggca gaaagtggct gagccaggag ggcggcctgt acttcagctt cctgctgaac
      181 cccaaggagt tcgagaacct gctgcagctg cccctggtgc tgggcctgag cgtgagcgag
      241 gccctggagg agatcaccga gatccccttc agcctgaagt ggcccaacga cgtgtacttc
      301 caggagaaga aggtgagcgg cgtgctgtgc gagctgagca aggacaagct gatcgtgggc
      361 atcggcatca acgtgaacca gagagagatc cccgaggaga tcaaggacag agccaccacc
      421 ctgtacgaga tcaccggcaa ggactgggac agaaaggagg tgctgctgaa ggtgctgaag
      481 agaatcagcg agaacctgaa gaagttcaag gagaagagct tcaaggagtt caagggcaag
      541 atcgagagca agatgctgta cctgggcgag gaggtgaagc tgctgggcga gggcaagatc
      601 accggcaagc tggtgggcct gagcgagaag ggcggcgccc tgatcctgac cgaggagggc
      661 atcaaggaga tcctgagcgg cgagttcagc ctgagaagaa gcggttctgg tggtgctagc
      721 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
      781 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
      841 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
      901 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
      961 tgggacctct attatcatgt gttccgacga atctcaaagc tcgagccacc aaaaaagaaa
     1021 agaaaagttt acccatacga tgttcctgac tatgcgggct atccgtatga cgtcccggac
     1081 tatgcaggat cttatccata tgacgttcca gattacgctg cttga
//
