LOCUS       sequence-447381-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BioID2-FKBP-NLS fusion from ADH1 promoter; yeast BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC
  TITLE     Characterization of BioID tagging systems in budding yeast and 
            exploring the interactome of the Ccr4-Not complex.
  JOURNAL   G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221.
  PUBMED    39271111
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221."
COMMENT     Sequence Label: pRS416-ADH1p- BioID2-FKBP-NLS-HA3
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="MSFKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRK
                     WLSQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKK
                     VSGVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRIS
                     ENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIK
                     EILSGEFSLRRS"
     CDS             721..1041
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1048..1068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1069..1158
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atgtccttca agaacctgat ctggctgaag gaggtggaca gcacccagga gagactgaag
       61 gagtggaacg tgagctacgg caccgccctg gtggccgaca gacagaccaa gggcagaggc
      121 ggcctgggca gaaagtggct gagccaggag ggcggcctgt acttcagctt cctgctgaac
      181 cccaaggagt tcgagaacct gctgcagctg cccctggtgc tgggcctgag cgtgagcgag
      241 gccctggagg agatcaccga gatccccttc agcctgaagt ggcccaacga cgtgtacttc
      301 caggagaaga aggtgagcgg cgtgctgtgc gagctgagca aggacaagct gatcgtgggc
      361 atcggcatca acgtgaacca gagagagatc cccgaggaga tcaaggacag agccaccacc
      421 ctgtacgaga tcaccggcaa ggactgggac agaaaggagg tgctgctgaa ggtgctgaag
      481 agaatcagcg agaacctgaa gaagttcaag gagaagagct tcaaggagtt caagggcaag
      541 atcgagagca agatgctgta cctgggcgag gaggtgaagc tgctgggcga gggcaagatc
      601 accggcaagc tggtgggcct gagcgagaag ggcggcgccc tgatcctgac cgaggagggc
      661 atcaaggaga tcctgagcgg cgagttcagc ctgagaagaa gcggttctgg tggtgctagc
      721 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      781 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      841 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      901 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      961 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     1021 gtggagcttc taaaactgga aactccacca aaaaagaaaa gaaaagttta cccatacgat
     1081 gttcctgact atgcgggcta tccgtatgac gtcccggact atgcaggatc ttatccatat
     1141 gacgttccag attacgctgc ttga
//
