LOCUS       sequence-447448-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide and lanthipeptide synthetase from 
            LanII-2A (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanII-2A
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7311..7358
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7399..7854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7486..7505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7696..7717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7946..8761)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7946..8761)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8669..8688
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8753..8772
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgtcaaacat tgatatcatt cgcgcctgga
     3601 aagacgagga ataccgtaac tcgttgtctg aggaacagct gtcacagttg ccagaaaatc
     3661 ccgcgggtat ggtcgaactg tccgacaagg aaatggagac gtttatgggt ggcggggatg
     3721 ttcatatcgc tatgtcgtgt tgtggcacta aagcgactaa ccagtcaaag aaactgccta
     3781 ttcaggatag tagcattctg gcagaagtgt cgtcattgga aaagggctaa gcgccattca
     3841 ggatcacgtt accgcgaaaa aaagggacca ggcggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttgccctta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatgtc aaaattttcg ttccataact cggtttggta
     4021 caactccatt acgctgactg aacgcatcat tagtttacag aacacccaga aagaaaccta
     4081 cattaccaat attgacacga aacttgccaa gaagaaaatg caacagtgga agagtcaatt
     4141 accgtttacc aatgattcgt tctttgcaca gcgtctgaca attgatcgca ttactgagga
     4201 cgatttcttc aatattctcg gcgaaccgat tgaaggcgtg aagaatcgtc tgccagacct
     4261 gccgcactgg ctgaaacagc tggcacaagc tttcttccac cctacccact ctcaggctaa
     4321 aattctgctg gccccggaga aactgggcga aggtaaggaa gctggcttcc tgtatatcat
     4381 cgagcccctt ctgagccagg aaattgatcg cctgaaacag ggaattcagg caatgtgcca
     4441 agcgcatagc gatttgccgt ttgaaccgag cacggtggtt tcgctcctct ttaccagcct
     4501 gcaggaacag ctggttaaca ttctgagccg taccatgatt cttgaactta atgtggctcg
     4561 tttacaggga ctgttacagg gcaacattcc gcaggaacgc tttcagtcct tcctgcaacg
     4621 cctgcgccag cgcgaaattg cgatttcgtt cctgcaggaa tatcccgtgt tggcgcgtca
     4681 ggtggttatt agcattaacc attgggtgaa tttcagcctg gaatttctcc agcacctcta
     4741 taacgattgg aacgtcattt gtgcaaaatt ctcgcccgaa accgacccgg gtgtgttggt
     4801 gcagatcgat agctcaattg ctgataccca ctgtgatgga cgtactgtaa ttattgccaa
     4861 attttccacc ggcttccagg tggtgtataa accccgttca ctggcgacgg acgaacattt
     4921 ccagcagctc ttattatgga ttaaccagcg cggcaaccac ccgcctttcc gcactctgaa
     4981 aatgcttaac cgcgataagt atggctgggt cgaatttgta gtggcgcaca gttgcaccac
     5041 cccggaagag gtgcaacatt tctaccagcg ccagggttca tatctggccc tgttgtatgc
     5101 tattaaagcg actgatttcc atatggaaaa tatcattgcc gccggcgagc acccaatcct
     5161 ggtggatctg gagagcttgt ttcatagcca aattgaagtc aatcacttaa acaagaaatc
     5221 agagcacctt gtggttaaaa cgtttaatta tagcgtgctc agcgtaggta ttctgcccca
     5281 gcgtctgtgg gagaataacg aatcgaaagg cgtagagatg tcaggcttgg gcggcgaaga
     5341 aggtcagtta acgcctagcc gtgtgcaata tctgaccggt attggaacgg acgaaatgcg
     5401 tgtggagctg aaacagatgc cgatgagcgg ttcccataac cgtccaacgc ttaattcggc
     5461 gccggtcaac atcctggatt acattgatgc cattaccgcc ggttttacta gcatgtacca
     5521 actgctgatc cagcaccgcc acgacctgct tagcgaaaag tccccgctgg cccgcttttg
     5581 cgaagatgaa gtacgcttta ttccccgttt cactgaggag tattcgctgc tgctgaatga
     5641 gagctaccat ccgaacctgc tccgtaatgc actggatcgt gatcgtttct ttgatctcct
     5701 ctggctgggc atcgaacacc agccgcatct ggccaaaatt atccatgccg agcgcgacga
     5761 tttgtggaaa ggcgacatcc cccgctttac aacccgcccg aactctaccg acctgtggac
     5821 cggctctaac gaaaagatta ctgatttctt cgacgaaacc ggaatggatc tcgttcgtcg
     5881 cagtctccat aacctgaacg acgcggattt acgtcagcag ctgtggttca ttcgcgcgag
     5941 ctttaccacg tttattatcc acaaggaaga aagtaaatgg cagggctggc cgattcacca
     6001 tttgccggag cccaagaatg actttagccg tgagcagctg gtagccgcgg cgcaagcagt
     6061 cggcgatcgc attgaggagc ttgccctgca agaagataac gacgtgtcct ggattgggct
     6121 gaacttcgat gagaaacgta attactggac cttcacgcct ctgggtatcg aattatacaa
     6181 cggtatgccg ggcattaccc tgttcttagc ttaccttggg actattacgc atcaaaagaa
     6241 ttatacagaa ttagcgaaac tgacgttagc caaattccag catcagattg atcgcaataa
     6301 aagcagcttc acttccattg gcggttttac ggggtggggt ggaattattt acactctgac
     6361 ccagctcggc gtgctgtgga acgaaccctc tctgctcctg gaagcagaat caattgttcc
     6421 tttattgccg gatctgatta gccaggacaa gtattttgat attattggcg gagcggccgg
     6481 ttgcttatca agccttatca gcctgtacca ctgtcgccca tcccagcgca ccttggatgt
     6541 tgcgattcag tgcggtgttc atatcattag ccacatccag ccgatgaaga aaggcgttgg
     6601 ctggatccca ggattgaaaa gcttatcaga gaaaccttta ccgggcttca gccacggtgc
     6661 agcgggtatc gcattagaac tgctggaact tgcaagttta acgggcgaga aacgctttca
     6721 caccacggcc ttggcggcaa ttgaatatga acgtagtctt ttctgtcgcc agtttggcac
     6781 ctggtgggat atttcgaatt tcagcgacat ggtccttgcg aactcatatc cgtgcatgac
     6841 cgcgtggtgc catggagcgc ctggcgtggg cctggcacgc ctgaaatgtt taccgtattt
     6901 ggacaacgcg gaaatccgtg acgagattta catcgcgctt aaaagcaccc ttgaacacgg
     6961 gtttggcctg aatcattccc tgtgtcacgg cgatctggga aacctggaac tgctcctgca
     7021 ggcttccttg attctgaaag acccccagtg gaagaaccag gttgatcgcc atgcagctat
     7081 tattctgagt tctattgaca aatacggctg gttatgtggc gttccgctgg aagtagaggt
     7141 accgggcctg atgacaggcc tcgccggtat tggatacgaa ttactgcgtt tggcggtgcc
     7201 ggaacgcatc cctagcgtgc tgatgctgga gcccccgcgc ttcaacaaaa cgagcgaaaa
     7261 gactcaggag attttctcac acaaaagcgc gattgaaatg gtgtaacggt ctagcataac
     7321 cccttggggc ctctaaacgg gtcttgaggg gttttttgag cgctgaaagg aggaactata
     7381 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7441 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7561 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7741 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7801 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7921 tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca
     7981 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8041 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8101 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8161 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     8221 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8281 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     8341 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8401 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     8461 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8521 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8581 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     8641 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     8701 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
     8761 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc
     8821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8881 tc
//
