LOCUS       sequence-447449-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide, lanthipeptide cyclase, and 
            lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanI-101
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5190..5237
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5242..5260
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5261..5285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      8576..8623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        8628..8646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8647..8671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      9157..9204
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9245..9700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9332..9351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9542..9563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9792..10607)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9792..10607)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10515..10534
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10599..10618
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgaaaaagca gctgagcaaa ctgaatctga
     3601 aaaccgataa ggtagtgaac ctgtcgaaag ataactccca gggcgtggta ggcggccgtc
     3661 cccagtcaaa cccgaacagc aaagtctatt gtcttagcga tctgtgtacg tttaccggcg
     3721 ccaccttttc ctgttgacta gaagcccggc agagttagtt cgaacctaat gccacaaatg
     3781 agacaggacg ccaatgggta ccggacatta ggtcgagctc agttcggtaa cggagagacc
     3841 ctgcggcgta cttaattatg catatgaaac gccggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttggctcta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatgct gacatcggaa gattttagta ctgactgtcg
     4021 taccattttg gatcgtatct atcaccacat tgccagtcag ccgacagatg ggcacgcgag
     4081 tttattaggc ggtcagatgg gttacgcgtt gtttgaagcg tattatcatc gccacttcgg
     4141 tattaaggat gatggccgta tttgggaacg cgtaagttca agcctgaatg atattcaaga
     4201 aggtaaatta atccatagct ttgcgggcgg catcgcgggt gttgcctggg gcttcttaca
     4261 tctcttcaat gaaggcttgc tgcaggacga tgaaattgat ccacagtcaa tcgtcgagga
     4321 cctggatgaa ggcctgtttg aatcggcgat gttgctcctg gcagatggca atttcgatta
     4381 ccttcacggc gccctgggtg tatgcttata tttcctggaa cgcacaccaa gcccgaaaat
     4441 tacgagctat atcgaccaga tcgttgaaca actctcttta gtggctgttc gctttccgtc
     4501 tggctcaatc acatgggaat ttgatgattt tggacgtcgt ggagcttcgt caccgccgct
     4561 gtacaacctg gggctgtctc acgggacagc ttccattgtt gcgattttga ccctgttgta
     4621 cgagaaaggc catgccccgg cgccttgcaa ggagctgatc gcaggcacct tgcgttggat
     4681 gtggaacgta cgtaacaaat cagcgcagtc ggtgtttcct tccgtaatcc aggcgaacgc
     4741 gactgatcag gaatcacgcc tcgcctggtg ttacggcgat ttgggaattg cgaacacgtt
     4801 cttattggcc ggccagaaac tcaagaacga tttctggatt gaaattgccg agcagacgat
     4861 tatccgtgcc tcagcgcgtc tgggccgcgg cgtagcgatg aaagacgcgg cgctttgcca
     4921 tggtacgctg ggcgccgcat atttgtttgg caaatttgca gaggcgtttc cgcagcactt
     4981 attggaacct acggaaacct actggttaag caagactttt gactatgtac aaccgacgga
     5041 gtcctctgac gttttcttat cttataacgg cgaacgctac gaatctaact taagcctttt
     5101 agatggtgaa gcgtccatcg ggatggcgct cttgacatac ttgggtgcta gtcgtgcctg
     5161 ggaacgtttc ttgctgttgt cctaacggtc tagcataacc ccttggggcc tctaaacggg
     5221 tcttgagggg ttttttgcgg ttaatacgac tcactatagg ggaattgtga gcggataaca
     5281 attccaagga gatatacaat gccactgctt aaatcgaaga agcctcgctt cgcgcccctg
     5341 tttcttagcc aggccgactc ccccgccaat tttaccaaga aatcagcgac cgtcatgtcc
     5401 actacctact tcagcaccgc cttcgtgcgt gtcccgctgg ccggctcgga agcaattgaa
     5461 aatatcgatt gggagaaatt atcgcgtcaa ctgactcagc cggctttcct cgaggcgctg
     5521 tatattgctt ctcccgccct ttacgaggaa gtacagaaac tggacgtaac ccagcaaccg
     5581 gacgaaaagt ctaaacgcat tatttattcg ttaatcaaat atttggcccg ctactcaact
     5641 cgctgcactc cgtttggctt atttggcgga tttgcgacgt taccgatttc aactgcgtcg
     5701 acagcggtgc gcattgacaa catcggcact agccgtaagg tgacccgcct cgatatgaac
     5761 tacctgtgcg ccctgaccca agatttagaa aagcaccctg acatcaagcc ctttctgcgt
     5821 ttctatccca atacgtcgtt atattcgatc aacggccaat atcgttatgt cgaatatcgt
     5881 tacaatggag gaggtatgcg cgtacaccac ttatctagcg ccgaacaatc agagtacttg
     5941 gaagcagtgc tcgcgcgtgc gcagaaaggc gcgccgctgt acgcgctggc agaagtgtta
     6001 gtgtcggaag aaattacgtt acaggaagcg ctggaatacg tagaagaggt agtggcgagt
     6061 cagctgctcg tcagcgagtt ggaaccggcc ctgaccggtg gtgatttcct tgcccagatt
     6121 attggtaccc tgcagaaaat tgcagacatt gaaacttcgg agcatttggc ggctattctg
     6181 tccttgctcg gagaagtcca gcaagcgctt ctcgtgattg agcagtcggg ttctgactat
     6241 ggttcagcaa aatttgaaaa ggtcgaggcc ttgttgagcc aactgccgac cccgtttgac
     6301 cgcaaagttc tcttccagat tgatacctac ctgccggaac tgagcggcca actgaaccgt
     6361 ggtctgctga acaaactggc gtccaagatt ccgactctgc tgaaattgag tccggcgggt
     6421 aaccctatgt tagacgaatt ccgcaaccgt ttctatgaaa agtatgagga cgaggaagta
     6481 ccgttcgtgt tagccttaga tcccgaagtg ggcattggct atccgggtgg tcaggccaag
     6541 aatgatatct cgccgctggt ggaaggcgtc tgggtgggca gtaaccagcc aagtaaccag
     6601 accttaacca ttccacccga acactcttat ctgttacaga aagtggccga ggcgcagctt
     6661 accggcgcgt atgaaatctg cttgaaggaa gaagatctgc gtgcggtgga agtccgccag
     6721 ccgcagctcc cgttgaccaa tacggcaatg ttctccgtaa ttcgcgaaga aggccgtgag
     6781 aagatcgtgc tcgatacttt cggcggtacc accggtacgt tcttagtcgg gcgttttggc
     6841 cataccgacg cttcagtgct ggcgttgctg caggaaatca gtgccgctga ggaccgttct
     6901 gcaccggaag tgatcttcgc cgatattgtt catctccctg aggcacgcac cggtaatgtg
     6961 attattcgcc cgcaattgaa gaaataccag attccgttcc tgtcaaaagc ctcaacggcg
     7021 ccggaatttg aaattgctct ttctgatttg atgctgtcgg tccgcggtgg caccatcttt
     7081 ctgcgctcca agtcattaaa caaaatcatc attcctcgct tgagcaacgc tcataatttt
     7141 agcgggaccc aatccttaga cacataccac tttctgtgcg ccctgcagag tcaacatgtt
     7201 cgctcagggc tctcaaactt caccggcagc ttttccagtt tgttcatgtt tattcctcgc
     7261 ttggttctcg ataacattgt tctcgccgaa gcgcagtgga atttcaagga acagcatgtg
     7321 aagagtctgg ttacagcttt taagaataac cactggccgg cccttaaagc tgaaatcgag
     7381 gcttggcgcg cgcagtacag cgtgcctcgc catatttgct taacccagtt cgacaatgag
     7441 ctgtacgtcg acctggaaaa ccagtggctg gcggaaacat ttctgaacga aatcaaaagc
     7501 ctggaacagt tcaacattaa agaattcctg catcgcgcgg actcgagcgt tgttcagagt
     7561 ggccgcgggt ggcacaccaa ccaatttgtt gtagcctttg ataacacagc tagctataaa
     7621 accgaaggtt tggcggctcc cgcactgcgt tttccaaaag cgaatcaagc aacacgcaaa
     7681 tttatcactg gttcagagtg gttatattat aaagtttaca cggggattaa aacagccgat
     7741 cgtttgttaa ccgaagtgct gtacccgctt accgagcgct ttaaggcaaa cggatggacc
     7801 gataagttct tctttattcg ctacgcggat ccgaatctcc attttcgcct tcgtttccac
     7861 ctggcaaacc cagcccacat cggccaagtc gtacaggaat tacacgatgc actggcaccg
     7921 tacatcgcga acaaaacgat cacctcggtc gttaatgaca cctataatcg tgagctggaa
     7981 cgctatggta gcaatagcat tgatgccatt gaggactatt tccatctcga tagtgataca
     8041 gtgttgcgtt ttctgacgat gattgaaggt tctgagggcg aggagtgtcg ctggcgtttc
     8101 ggcatgaagt tgacggatga cctgcttact gcatttggct ttgatatgaa acagaaaatg
     8161 gaatttacag aaaagcgcgc cgccgatttt ggccgcgaat tcggctataa cgctggtttg
     8221 aagaagcagc tggatgcgcg ctataaagac gtggaaccag ccctgcaaga gctgtttgcc
     8281 ctggctaatc cagaacacga attcttctat gagttatcga atcagcgcac cgaaaacttg
     8341 cagctcatga tcgaatatgt gcgcatgctg catgcgcaga gtgaactgca gattagcttg
     8401 gaagatcttc tggcctcact ggtccacatg aatgtgaatc gtttgtttcg ctcacagcag
     8461 cgtctggtag aatacatggt gttctatcat ctgcacaaat actatcgcat caattacggc
     8521 cgtaatgttc tggcgaagaa aacggcggac agcaacgtca tcgcgcagta acggtctagc
     8581 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttggtgctaa tacgactcac
     8641 tataggggaa ttgtgagcgg ataacaattc caaggagata tacaatggag aaactggatt
     8701 gccttaacca agtggccgag ttccatcgca cctttaaaca tccgattctt tcaatcccgc
     8761 aaatcccgtc tgaggaccgc tgcaatttgc gcgtggccct tatcgccgag gaactgaaag
     8821 aattagaggt ggcgatcctg gagaaagata tcgtgggcgt ggcagatgca ctggcagata
     8881 ttcagtacgt gttgagcggt gccgtgttgg agtttgggtt aggcgaacag tttaaagccc
     8941 tgttcgacga ggttcaacgc agcaatatgt ctaaagcatg taataccgaa gaagaggccc
     9001 aggccactgt caaacatttc caggataaag gtacggaatg ctactacgtt gaggatgccg
     9061 gtaaatattt agtctatcgt acagcggata ataaaactct gaaaagcatc ttttattcgc
     9121 cagcggatct ggaaggcgtg ttgaagaagt gacggtctag cataacccct tggggcctct
     9181 aaacgggtct tgaggggttt tttgagcgct gaaaggagga actatatccg gattggcgaa
     9241 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9301 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9361 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9421 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9481 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9541 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9601 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9661 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9781 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     9841 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     9901 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     9961 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
    10021 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
    10081 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
    10141 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
    10201 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
    10261 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
    10321 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
    10381 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
    10441 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
    10501 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
    10561 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta
    10621 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt
    10681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
