LOCUS       sequence-447450-        8852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide and lanthipeptide synthetase from 
            LanII-2C (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanII-2C
FEATURES             Location/Qualifiers
     source          1..8852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7281..7328
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7369..7824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7456..7475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7666..7687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7916..8731)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7916..8731)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8639..8658
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8723..8742
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgtctaacct ggacattatc cgcgcctgga
     3601 aagacgaaga atatcgcaac tcgctctccg atgaacagcg cgcgcagctg ccggagaatc
     3661 cggcgggtat gattgagctg tccgatgaag atatgggtgc cattagtggc ggatttgcgg
     3721 cgtctcgttc ggtgtctcgt caaggtggct gttcatgcag ttgtggcggc aattgttgtt
     3781 ccaaaccgca aatccaaaac attgccgcgt tactgtaaga aatactaggt aactacaggg
     3841 actccgacgt tatgtacgtt gctccgtcag agcggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttgccctta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatgca tcagaccagt ttccagagta gcgcgtggta
     4021 caatgccatc ccgttgaccg aacgtattac ctttcttcgc actcaacagt cgaaaacgcc
     4081 gaaagtggaa gtcaacgtgg ccttggccca acgtcgcatg caacgttgga aaagccagtt
     4141 cccgtttacc aatgattcgt attttgatca gcgtctgacc atggacgcta ttacggaaaa
     4201 ggaattcatc tacctcttag gtgaacctac cgatgttatg aaaacacgct tttcggctat
     4261 cccggactgg ctgacagatt tggctcaagc attcttccac cctacttatt ctaacgcaac
     4321 cattatcctg ccgccacaaa agcttggcga gcagaaggag tcaggtttcc tgtatgccat
     4381 tgaacctctg atttcgcagg gtctggatcg tctgcacgaa ggcatccaga aacttattca
     4441 aacttatcag aacttacctt tcgatccgac gacgatcaaa gcactgctgt tcgcaaactt
     4501 acccggcgaa ctgttatgga tgctgaaccg caccatgatc ttggaattga atgtggcacg
     4561 cttacaggga ctgctggttg gcgatctgcc ggaagatcgt tttcagtcgt ttctggagcg
     4621 tctgcatcag cacgaggtgg tgatcttcat tctgaaagaa tatccggtcc tggcacgcca
     4681 acttgtgatt agcatcaatc agtgggtgtc ctactcgctt gaattcttgc agcatctttg
     4741 cactgattgg agtgatatcc gtacaacctt tagcccggaa gcggaaaccg gcgttatggt
     4801 gcaaatcgac ggcggtgtta gtgacacgca ccgtggcggt cgcagcgtgc tcattgccaa
     4861 gttctcgtcg ggattccaga ttgtgtataa accgaaatcg ctggccgttg atgttcactt
     4921 ccaacagctg ctggaatggc tgaaccagcg tggcaattat ccacctttcc gtaccattaa
     4981 aatcattaat cgtggcactt acggctgggt tgaattcgtc acggcaaaag gatgcaatga
     5041 tccggaatgt ttacagaatt tctacaagcg tcagggtggc tatctggcgc tgctttatgc
     5101 attagaagcc acggattttc acctggagaa tttaattgcc gttggcgaac acccggtgtt
     5161 agtggatctt gagagcttat ttcatccgcg cattgaatct atcgatatca agaagagcga
     5221 acagctggcc atcaacacca ttgataatag tgtactccgc gtgggcctgc ttccacagcg
     5281 cttttgggcg aatgcggaat cggaaggtgc ggagatctct ggcttgggtg ggaaagaggg
     5341 tcagcttacg ccgcatcgcg taagctactc tgaagagatt ggtaccgacg aaatgcgtgt
     5401 ggcccgcaaa caaatgccca tgagcggctc gcaaaaccgc ccgactttga atgatgcaga
     5461 agttaacgtt ctggactata ccgaagcgat catcacaggc tttaccaata tttatcaact
     5521 tattcttcag taccgtgacg aattgcttag tgaaaatagc ccgctggcac gcttcgccga
     5581 agatgaagtg cgctttcttc tgcgccaaac gcgcagctat ggcctcttgt tacacgagtc
     5641 atttcatccc aatctgctgc gcaatgcact ggatcgtgat cgcttctttg accgtctgtg
     5701 ggttggcatt gaaaagcaac cgtatctgac caaggtgatc gcggccgaac gcgatgattt
     5761 atggcagggc gacattccga tgtttacaac ccgcccgaac tcgcgcgcta tctggtcatc
     5821 tttcaataag aaaattgcgg atttctttga tgagacgggc atgaccctcg tccagcgtcg
     5881 tattcagcaa ctgtcaaacg cggatttgaa acagcagatt tggtttgtgc gcgcatccct
     5941 ttcaacctta gcaatggccg aagaacaggc gaaatggcct acctatcatc tgaataaacc
     6001 ccaaaacagc gcgtcttatg agcagttggt tgccgcggcg caggtcgtag gtgaccgctt
     6061 agagaaactg gcgttaaacg gcgaaaagga tatttcatgg attggtctga cactgatcga
     6121 aaagcactgg acattaaccg cactggaaat tgatttgtac gaaggcttac cgggcgttat
     6181 cttgtttctg gcgtatcttg gcgcgcttac cgaacagaag cgctacacgg ccttagcgaa
     6241 agcggcgttg gtaaccatgc aacgccaggt cgaatccagc cgcgagtaca tcaaatcact
     6301 gggcggattt agcggttggg gcggcgtgat ttacacgctg acccaattag gagtgctttg
     6361 gaacgaaccg gagttattga ccgaagcgga atctttggta gaacagttcc cgaacctcat
     6421 ttcaaaagat gagcaactgg acattttgtc tggtacggct ggtgggcttg cctcgctgat
     6481 ctccctgtat cgttgccgtc cgtcgcagcg caccctcgcc gcagccattc aatgcgggga
     6541 acgcctgatc acaaccgcaa aaccgatgga gtatggtgtg ggctggacaa cccagatcgc
     6601 ggaattaaaa ccgttaaccg gttttagcca tggcgttgcg ggtatcgcgt gggttcttct
     6661 ggagctcgcg tccctgaccg gcgaacagcg ttttaaaact accgcgcttg cagcaattga
     6721 atacgagcgt tcactgttcc gccctgaggt gggtaattgg cctgacctgc gcgatttctc
     6781 aagctccatc ttggcggaca aagatgataa ccagccgacc tgtatgacgg catggtgcca
     6841 tggagccccg ggcatcggtc ttggccgcct gcgtagtctg cctcacttag atgacgcgaa
     6901 gatccgtacc gagattgata ccgctcttaa gacgactctg gagcatggtt ttggctcgaa
     6961 ccactcgctg tgccacggcg acctgggcaa cctcgagctc ttgcttcagg caagccagac
     7021 ctttgatgat ccgcagtgga aaactcaggt ggatcgcttt gcggctatta tcttagaatc
     7081 tattgataaa tacggatggt tgtgtggcgt gccgctgggt gtcgaaaccc cgggtctgat
     7141 gaccggactg gcgggcatcg gttatggctt actccgtctg gctgcccccg atcgtgttcc
     7201 ttcagtactt gttcttgagc cacctaaatt aaatcgcgcc gtgcagaagt cgactgattg
     7261 cgcaattgcg atctaacggt ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     7321 gttttttgag cgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     7381 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7441 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7501 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7561 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7621 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7681 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7741 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7801 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     7921 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     7981 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     8041 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     8101 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     8161 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     8221 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     8281 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     8341 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     8401 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     8461 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     8521 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     8581 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     8641 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     8701 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     8761 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8821 tcagaccccg tagaaaagat caaaggatct tc
//
