LOCUS       sequence-447451-        8900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses precursor peptide and lanthipeptide synthetase from 
            LanII-56 (FAST-RiPPs) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Ayikpoe RS, Shi C, Battiste AJ, Eslami SM, Ramesh S, Simon MA, 
            Bothwell IR, Lee H, Rice AJ, Ren H, Tian Q, Harris LA, Sarksian R, 
            Zhu L, Frerk AM, Precord TW, van der Donk WA, Mitchell DA, Zhao H
  TITLE     A scalable platform to discover antimicrobials of ribosomal origin.
  JOURNAL   Nat Commun. 2022 Oct 17;13(1):6135. doi: 10.1038/s41467-022-33890-w.
  PUBMED    36253467
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-17"; volume: "13(1):6135. doi"; pages: " 
            10.1038/s41467-022-33890-w"
COMMENT     Sequence Label: pET28:LanII-56
FEATURES             Location/Qualifiers
     source          1..8900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3489..3507
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3508..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3549..3566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3877..3924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3929..3947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3948..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7329..7376
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7417..7872
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7504..7523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7714..7735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7964..8779)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7964..8779)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8687..8706
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8771..8790
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaatatggta atacgactca ctatagggga attgtgagcg gataacaatt ccaaggagat
     3541 atacaatgca ccaccaccac caccacgcaa tgcaaccaac ggcggaacag gtacgtgctt
     3601 taaaagatga ggattttcgt ctgactttgg ggcaaggtca ccaagtgcat cccgcaggtg
     3661 aaccggcgga tgaagtttat gttactggtg cggtgcaggc gtcgacgtgt ggcggctggt
     3721 cgtcttgtgg ctcggtgtgt agcttttaaa gagtcaactt caccacatat gctgtctctg
     3781 gcacgtggat ggtttagagg aatcagattc aagtctggtt aaccatcaaa caggtcttga
     3841 gtctaaaatt gtcgtctcct gcgtacgaga tgcggtctag cataacccct tggggcctct
     3901 aaacgggtct tgaggggttt tttgccctta atacgactca ctatagggga attgtgagcg
     3961 gataacaatt ccaaggagat atacaatggt tgacgctagt cattttcttc gtgccgccgc
     4021 tcgtgccgtt ccactgcatg cacgcggccg cgccgctcct gatggcactg gcccacatag
     4081 cgaagcggca ggattagccg aacgtgcagc acgtgctgcg cgtaaattgg aacgttggcg
     4141 ttcccaggct ccctttgatc gtgatggttg gtttgaacgt cgtcttgctg ctgatggatt
     4201 aactccggaa gcattaggag cattattggc cgaacaccct gatgcgttag ctgatcgtct
     4261 gggtccgcca ccttgggcgg aacgtttagc agaagcattt cgctcagaag ctgaacctga
     4321 tgcgcctgat gctcaagcgg aaccagcagc cgtagtagcg ccagctgccc ctgccgatcc
     4381 ggccgcgcct gcggcccgtg ccgtactgga cgtacctgaa gaacatgctt ttgcacgttt
     4441 agttgcagct ttactcgccg acgcgcgcga tcgcctctgc gcgggaattg ccgctctgga
     4501 tgcaccagcc cttggggctc cggcggaact tactgcatta gcattacctg cattaacagc
     4561 agccgcacac ggtttagcca cccgtacagt cattactgaa ctgcacgttg ctcgtttaag
     4621 cggagaattg acaggagaca ccccggaaca gcgttatcgt gattttctgg gccgtttggc
     4681 gcgcccagaa cgcgcgctgg gtttactggc ggaatatcca gtactggcgt atcgtttagc
     4741 taccgcagca gatcaatgga ctaataccac attaaccctt ttacgtcgtt tagctgcgga
     4801 tctgccggcg ttacgtgcag cgtttgcaga tggtcgtccg ttaggtcgtt taactgaact
     4861 ggcgaccggc ttaggcgatc cccatcgcgg tggtgcgacc gtttctgcat tacgttttga
     4921 acatggtgtt cgtatcgttt ataaaccacg tcctcttggg gcggaaaccc attttcgcca
     4981 attgctccaa tggctgaacc cacagctccg tcgtcctctt ttggatttag cccatgtggc
     5041 aggtgatggt tatggttggg tggcgcatct gacagcagaa cccacacgta gtgctgcaga
     5101 gttgagcgat ttctatcatc gtgcaggcac tttagcagca cttatgtatg cattagatgc
     5161 agtcgattgt catgcgcaaa atttactggc ggtacgtgac caaccggttt taattgacct
     5221 tgaagcactg ttacatcctg atttgcatga gccggctaag gatttgtcag cttcagaacg
     5281 tttggctcgt catcgtgcac gccattcagt tctccgttcg ggactgctgc ctgaacgtat
     5341 gtgggatgag ggcaatggtg gaacggattt gagcgcattg ggttgggatc ccgcaggact
     5401 cccgccgcgt ccggttccgg tactggtggg tcaaggtact gatgaattac gtctggaata
     5461 tcgtcgtagc cctgttgaag cgcctggttc acgtccagta cctgcaggtc atcctttgcg
     5521 tccagccgat catttagcgg aactggaagc cggatttact gaagcgtatc gtttattagc
     5581 ggcgggtaaa gaccgtctgc gtaccctgct ccgtgctttc gcggccgatg aaactcgtat
     5641 gctgcgtcgt gacacttttg aatatcgttc tttgttgaat gcagcatgtc atcctgattt
     5701 acttcgtgat gcgttagatc aggatcgtca tctggatcgt ctgtgggcat tagcgggttg
     5761 ggaagaacat gcattggctt ttgttgaaca cgaacgtgcc gatttatggc gtggtgatgt
     5821 tccagttttc actattcgtc cggatggtgt cgatgcacgt agcgctaccg gtgccgttat
     5881 tcctggcgca gcagatcgcc cagcgcttga cttggcactg gcaaaagcag acggtttagg
     5941 tgaggacgat ctggcacgcc agttacaatt acttcgtctc tccgtcacca ctgctgatgg
     6001 tagtcgtgaa ccccgtcgcg cagctttagc ggtcgcagga gatcaaccag cagcgagtga
     6061 tggactgccg ccagaagcgg gccaacgcat tgcggcacgt gccctcgcaa aagccgtaga
     6121 ggcaggagaa cagttactgc gtgaagccta tcgtggcgga gcagatttag cctgggcagg
     6181 accaaattgg acaccaccag gtcgttgggc cccggccgag cttggcccgg acttgtattc
     6241 cggaactgcc ggcattgctc tgttcttaca tcaactggcg gtagttactg gagatcgtga
     6301 acatgaagaa gcggccggtg cagcggaact gaccttacgt catcaagtcc gtcgtcgtgc
     6361 tgatcgtttg ggtggcggat tagcaggtgc tggtggtgtg ttatatgcgt tagcacatct
     6421 tgctgcagat cgtccgggtg cagtacaccc agcaactgat cgtagcgcgg cagttaatcg
     6481 ccgtgcagaa ctggatgcat tagccggact tgttattgat cgtgtcgcag cgacagcccc
     6541 cgaagatacg ctgcatgact tactctccgg aaatgcaggc acaattggtg gtctgctggc
     6601 atgggaagcg gttcgccctg gtaaagcggt aacggctgcg cttgtagcat gtacagaacg
     6661 tctggcccgt agtgcagcgc cgcatcctga tggcggtgtt ggttggcttc ctgcatcttt
     6721 agccggtgtt gcccgtcgtc ctcttgcagg ttttgcacat ggtggttcag gtgttgcatg
     6781 ggcgttagcc cgcgctggtc gtcgtttagg tgatcgtgaa ctctcagaat tagccttggc
     6841 cgccgttgaa tatgaacgca cacttttcga tcccgcagcg gctaattggc gcgatgttcg
     6901 tgaagatgat ggtccacatg ccccgagctt tccggcatta tggtgtcatg gtgcgagtgg
     6961 tattgcatta gcacgcacag aattagccga actcctgtcg gatgatgtgg aattgctccg
     7021 taaagaacgt gatacggcac tggataccgt tcgccgtgaa ggtttcggtc ttaacttttc
     7081 cctctgtcat ggcgattttg gaaatttgga agcattacat ttagctggag caccttggca
     7141 tgctccggca ctcgcgactc ttgatgcact cgatgcacgt ggttgggttt gtggactgcc
     7201 acacggcgtt catagcccga gccttatggt tggtttggcc ggcattggcc atggcctgct
     7261 tcgtttagcc gcgcctgaac aggtgccaag cgttctggcg ctccatccgc ctacggcttt
     7321 ttaacggtct agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgagcg
     7381 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     7441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7861 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7921 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     7981 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     8041 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     8101 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     8161 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     8221 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     8281 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     8341 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8401 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     8461 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     8521 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     8581 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     8641 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     8701 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     8761 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     8821 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8881 gaaaagatca aaggatcttc
//
