LOCUS       sequence-447483-        2151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-BBa_B0012
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..82
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(86..129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    132..151
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      188..215
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      256..313
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(337..354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(508..1096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(588..607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1279..1373)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1394..2053)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1968..1987
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
       61 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      121 gatgatttct ggaattcgcg gccgcttcta gaatctagag acgcatatgg gtctcatcga
      181 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atgttagaga ccgcatgccg
      241 tctcattagc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      301 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
      361 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      421 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      481 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
      541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1261 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1321 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1381 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1441 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1501 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1561 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaca
     1621 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1681 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1741 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1801 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1861 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1921 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1981 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2041 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2101 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc c
//
