LOCUS       sequence-447494-        2160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L3S1P11 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S1P11
FEATURES             Location/Qualifiers
     source          1..2160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..82
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(86..129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    132..151
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      265..322
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(346..363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(517..1105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(597..616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1288..1382)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1403..2062)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1977..1996
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
       61 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      121 gatgatttct ggaattcgcg gccgcttcta gaatctagag acgcatatgg gtctcatcga
      181 gacgaacaat aaggcctccc ttcggggggg ccttttttat tgatacaaaa tgttagagac
      241 cgcatgccgt ctcattagct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc
      301 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc
      361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
      541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1261 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     1321 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1381 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1441 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1501 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1561 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1621 tggctgacac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1681 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     1741 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     1801 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     1861 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     1921 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     1981 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2041 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2101 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
//
