LOCUS       sequence-447497-        2171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L3S2P21 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S2P21
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..82
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(86..129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    132..151
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      276..333
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(357..374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(528..1116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(608..627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1299..1393)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1414..2073)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1988..2007
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
       61 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      121 gatgatttct ggaattcgcg gccgcttcta gaatctagag acgcatatgg gtctcatcga
      181 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
      241 ctgttagaga ccgcatgccg tctcattagc tgcagtccgg caaaaaaggg caaggtgtca
      301 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1261 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
     1321 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1381 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1441 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1501 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1561 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1621 cacccaggga ttggctgaca cgaaaaacat attctcaata aaccctttag ggaaataggc
     1681 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1741 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1801 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1861 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1921 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1981 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2041 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2101 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2161 tcttacgtgc c
//
