LOCUS       sequence-447498-        2799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eCFP"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eCFP
FEATURES             Location/Qualifiers
     source          1..2799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2..4,5..7,8..520)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      728..785
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(809..826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(980..1568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1060..1079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1751..1845)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1866..2525)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2440..2459
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2687..2705
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2709..2752)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2755..2774
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
      481 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      601 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      661 cctgctggag ttcgtgaccg ccgcctaagg atcctcgaag agacccatgc cgtctcatta
      721 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      781 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      961 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1741 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1801 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1861 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1921 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1981 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     2041 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga cacgaaaaac
     2101 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     2161 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     2221 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2281 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     2341 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2401 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2461 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2521 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     2581 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     2641 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2701 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     2761 gcggccgctt ctagaatcta gagacgcata ggtctcaca
//
