LOCUS       sequence-447503-        2130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-pET-RBS
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..82
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(86..129)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    132..151
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      235..292
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(316..333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(487..1075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(567..586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1258..1352)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1373..2032)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1947..1966
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
       61 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      121 gatgatttct ggaattcgcg gccgcttcta gaatctagag acgcataggt ctcagtactt
      181 taactttaag aaggagatat acataagaga cccatgccgt ctcattagct gcagtccggc
      241 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      301 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      481 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      901 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1141 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1201 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     1261 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1321 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1381 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1441 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1501 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1561 aatcaaaact ggtgaaactc acccagggat tggctgacac gaaaaacata ttctcaataa
     1621 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1681 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1741 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1801 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1861 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1921 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1981 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2041 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2101 tatggtgaaa gttggaacct cttacgtgcc
//
