LOCUS       sequence-447564-        6471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mutated L. gasseri gusA from strong Ppgm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Duong T, Miller MJ, Barrangou R, Azcarate-Peril MA, Klaenhammer TR
  TITLE     Construction of vectors for inducible and constitutive gene 
            expression in Lactobacillus.
  JOURNAL   Microb Biotechnol. 2011 May;4(3):357-67. doi: 
            10.1111/j.1751-7915.2010.00200.x. Epub 2010 Sep 1.
  PUBMED    21375708
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1751-7915.2010.00200.x"; journalName: "Microb 
            Biotechnol"; date: "2011-05"; volume: "4"; issue: "3"; pages: 
            "357-67"
COMMENT     Sequence Label: pTRK892
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2924..2935)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgataaaatc ttgctcaata atcacccaat ttatttaaaa ggctttggca agcacgaaga
       61 ttttaatgtt ttaggcaaag cagttaacga aagcattatc aaacgcgact acgaatgcat
      121 gaaatggatt ggcgctaact gttttagaag cagtcactat ccttacgccg aagaatggta
      181 tcaatatgcc gataaatatg gctttttaat tattgatgaa gtacccgctg ttggtcttaa
      241 tcgttcaata actaactttc ttaatgtaac taattctaat cagtcgcact tttttgcttc
      301 gaaaactgtg cctgaattaa aaaaggtcca tgaacaagaa ataaaagaaa tgatcgatcg
      361 cgaccagcgt cacccttcag tgattgcctg gagtttattc aatgaaccag aatcaactac
      421 tcaagaatcc tatgactatt tcaaagatat ttttgccttt gcgagaaaat tggatccaca
      481 aaatcgtcct tatactggaa ctttagttat gggtagcggt ccaaaagtgg ataagcttca
      541 cccactttgt gactttgtct gcttaaaccg ttattatggt tggtacgttg ctggtggtcc
      601 tgaaatcgtt aatgctaaaa agatgctgga agatgaacta gacggctggc aaaacttaaa
      661 gcttaataaa ccatttgtct ttactgagtt tggcgctgat acattatctt cttctcatcg
      721 ccttccagat gaaatgtgga gccaagaata tcaaaatgaa tattatcaaa tgtattttga
      781 tatatttaag aaatatccat ttatttgtgg cgaattagtt tggaactttg ctgactttaa
      841 gacgagtgaa ggaatcatgc gtgttggtgg taacgctaaa ggaattttta ctcgcgatcg
      901 tgaacctaaa gatattgcct ttaccttgaa aaagagatgg caacaattaa attaatattt
      961 tagtttttac aaataatcgt gctcaattaa aaataatcga tatcatttta gttcatttga
     1021 tatcgattat ttgattatgg gcgcgatttt ttattgattt tgataataat ttctaactaa
     1081 gaaatgtttc attaatttat gaaactaata tcttgtttct taaacaaatc atatacaact
     1141 aagtctgatg aatccatcta gagcggccgc tctagaagct tatacactga taataacatc
     1201 aagacgcaat tttatacaac gttaaaaata tcctttttcg cactttaagt ttgttatttt
     1261 aatctaaaga aaaaagagac tagtatacta gcctcctttt tcttcagatt atttttttcc
     1321 atgctctcta gcatatttgg ccatctgaat acctgcttgt cgaccaaaaa tcacagtttc
     1381 agcaatagaa ttatctagat tcaggaattg tcagataggc ctaatgactg gcttttataa
     1441 tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc
     1501 gttagttgaa gaaggttttt atattacagc tccagatcga ttcacaaaaa ataggcacac
     1561 gaaaaacaag ttaagggatg cagtttatgc atcccttaac ttacttatta aataatttat
     1621 agctattgaa aagagataag aattgttcaa agctaatatt gtttaaatcg tcaattcctg
     1681 catgttttaa ggaattgtta aattgatttt ttgtaaatat tttcttgtat tctttgttaa
     1741 cccatttcat aacgaaataa ttatactttt gtttatcttt gtgtgatatt cttgattttt
     1801 ttctacttaa tctgataagt gagctattca ctttaggttt aggatgaaaa tattctcttg
     1861 gaaccatact taatatagaa atatcaactt ctgccattaa aagtaatgcc aatgagcgtt
     1921 ttgtatttaa taatctttta gcaaacccgt attccacgat taaataaatc tcattagcta
     1981 tactatcaaa aacaattttg cgtattatat ccgtacttat gttataaggt atattaccat
     2041 atattttata ggattggttt ttaggaaatt taaactgcaa tatatccttg tttaaaactt
     2101 ggaaattatc gtgatcaaca agtttatttt ctgtagtttt gcataattta tggtctattt
     2161 caatggcagt tacgaaatta cacctcttta ctaattcaag ggtaaaatgg ccttttcctg
     2221 agccgatttc aaagatatta tcatgttcat ttaatcttat atttgtcatt attttatcta
     2281 tattatgttt tgaagtaata aagttttgac tgtgttttat atttttctcg ttcattataa
     2341 ccctctttaa tttggttata tgaattttgc ttattaacga ttcattataa ccacttattt
     2401 tttgtttggt tgataatgaa ctgtgctgat tacaaaaata ctaaaaatgc ccatattttt
     2461 tcctccttat aaaattagta taattatagc acgagctctg ataaatatga acatgatgag
     2521 tgatcgttaa atttatactg caatcggatg cgattattga ataaaagata tgagagattt
     2581 atctaatttc ttttttcttg taaaaaaaga aagttcttaa aggttttata gttttggtcg
     2641 tagagcacac ggtttaacga cttaattacg aagtaaataa gtctagtgtg ttagacttta
     2701 tgaaatctat atacgtttat atatatttat tatccgattt tttattaaaa cgtctcaaaa
     2761 tcgtttctga gacgttttag cgtttatttc gtttagttat cggcataatc gttaaaacag
     2821 gcgttatcgt agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa gatgttgtct
     2881 gttagattat gaaagccgat gactgaatga aataataagc gcagcgccct tctatttcgg
     2941 ttggaggagg ctcaagggag tatgagggaa tgaaattccc tcatgggttt gattttaaaa
     3001 attgcttgca attttgccga gcggtagcgc tggaaaattt ttgaaaaaaa tttggaattt
     3061 ggaaaaaaat ggggggaaag gaagcgaatt ttgcttccgt actacgaccc ccccattaag
     3121 tgccgagtgc caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttaagg
     3181 ggtttttcaa tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat
     3241 ttaagtagct ttattgttgt ttttatgatt acaaagtgat acactaactt tataaaatta
     3301 tttgattgga gttttttaaa tggtgatttc agaatcgaaa aaaagagtta tgatttctct
     3361 gacaaaagag caagataaaa aattaacaga tatggcgaaa caaaaaggtt tttcaaaatc
     3421 tgcggttgcg gcgttagcta tagaagaata tgcaagaaag gaatcagaac aaaaaaaata
     3481 agcgaaagct cgcgttttta gaaggatacg agttttcgct acttgttttt gataaggtaa
     3541 ttatatcatg gctattaaaa atactaaagc tagaaatttt ggatttttat tatatcctga
     3601 ctcaattcct aatgattgga aagaaaaatt agagagtttg ggcgtatcta tggctgtcag
     3661 tcctttacac gatatggacg aaaaaaaaga taaagataca tggaatagta gtgatgttat
     3721 acgaaatgga aagcactata aaaaaccaca ctatcacgtt atatatattg cacgaaatcc
     3781 tgtaacaata gaaagcgtta ggaacaagat taagcgaaaa ttggggaata gttcagttgc
     3841 tcatgttgag atacttgatt atatcaaagg ttcatatgaa tatttgactc atgaatcaaa
     3901 ggacgctatt gctaagaata aacatatata cgacaaaaaa gatattttga acattaatga
     3961 ttttgatatt gaccgctata taacacttga tgaaagccaa aaaagagaat tgaagaattt
     4021 acttttagat atagtggatg actataattt ggtaaataca aaagatttaa tggcttttat
     4081 tcgccttagg ggagcggagt ttggaatttt aaatacgaat gatgtaaaag atattgtttc
     4141 aacaaactct agcgccttta gattatggtt tgagggcaat tatcagtgtg gatatagagc
     4201 aagttatgca aaggttcttg atgctgaaac gggggaaata aaatgacaaa caaagaaaaa
     4261 gagttatttg ctgaaaatga ggaattaaaa aaagaaatta aggacttaaa agagcgtatt
     4321 gaaagataca gagaaatgga agttgaatta agtacaacaa tagatttatt gagaggaggg
     4381 attattgaat aaataaaagc cccctgacga aagtcgaagg gggtttttat tttggtttga
     4441 tgttgcgatt aatagcaata caattgcaat aaacaaaatg atcttccttc aggttatgac
     4501 catctgtgcc agttcgtaat gtctggtcaa ctttccgact ctgagaaact tctggaatcg
     4561 ctagagaatt tctggaatgg gattcaggag tggacagaac gacacggata tatagtggat
     4621 gtgtcaaaac gcataccatt ttgaacgatg acctctaata attgttaatc atgttggtta
     4681 cgtatttatt aacttctcct agtattagta attatcatgg ctgtcatggc gcattaacgg
     4741 aataaagggt gtgcttaaat cgggccattt tgcgtaataa gaaaaaggat taattatgag
     4801 cgaattgaat taataataag gtaatagatt tacattagaa aatgaaaggg gattttatgc
     4861 gtgagaatgt tacagtctat cccggcaata gttaccctta ttatcaagat aagaaagaaa
     4921 aggatttttc gctacgctca aatcctttaa aaaaacacaa aagaccacat tttttaatgt
     4981 ggtcttttat tcttcaacta aagcacccat tagtagatct tattaagatt accgttatcc
     5041 gtgaaaaacg agtggtagca attgctaaat aacaaaaaga gtatgagttt ttgctcatac
     5101 tctttttgtt atttgtgcaa ataccgctct acttgtataa ttagaacaag tatataagga
     5161 aagtagccga atatgtttaa aattattgtt gaattattct tattagtaat tatttcagct
     5221 gcaattacgc catttattag agatctatcg atgcatgcca tggtgcgaca agtaataaac
     5281 taaacaaaac aactacaaaa tatttctttt tgtttttcat gatttttaca cttctcttag
     5341 tatgcttttg ttataagtta gcacaaaaaa gcagaaaata aaaagtagaa ataaaaaaag
     5401 atgttttttt gcccatatct ctatgaaaaa aactgtgaaa tgtgtaaaat atggatgaaa
     5461 cattgaattt aaaaggagat atttcatgtc aaaattagtt ttaatccgtc acggtcaaag
     5521 tgaatggaac ctttcaaacc aatttactgg ttgggttgac gttaaccttt cagaaaaagg
     5581 tgttgaagaa gctaagaagg ctgaattcta ctagaaagga aaatcatcta tggaatctgc
     5641 actatatcca attcaaaata aatatcggtt taacacttta atgaatggca cttggcaatt
     5701 tgaaactgat cctaactctg ttggtcttga cgagggatgg aataaagagt tgcctgatcc
     5761 tgaagaaatg cctgtaccag gtacgtttgc agaattaact actaagcgag accgtaaata
     5821 ctatactgga gacttttggt atcaaaaaga cttctttatt ccttcatttc taaagaagaa
     5881 agaactttat atccgttttg gttcggttac tcatcgcgca aaagtattta ttaatggaca
     5941 tgaagtcggt caacatgaag gtggtttttt accatttcaa gtaaaaattt caaattatat
     6001 taattacgac caaactaatc gtgtaactgt tttagtcaat aacgaattat ctgaaaaagc
     6061 tattccttgc ggcaccgaag aaatcttaga taacggtcaa aaacttgctc aaccttattt
     6121 tgatttcttc aattattctg gcattatgcg gaatgtctgg ctcttagcac ttcctcaaag
     6181 ccaaatcact aattttaaac taaattatca attagcaaat aataaggcaa caattaccta
     6241 caatatcgag gcaaataata atgctgaatt taaagtaaca cttttcgata atcaaaaaga
     6301 agtagcgtgt gctacttcta aaaatactag tagtttaaca attaagaatc cgcacctttg
     6361 gagtccaaac gatccgtatt catacaaaat aaagattgaa atgctcgaag acggaaaaac
     6421 agttgacgaa tacacagata aaattggtat ccgcacagtt aaaattgtga a
//
