LOCUS       sequence-44757-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Beach JR, Licate LS, Crish JF, Egelhoff TT
  TITLE     Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II 
            assembly and function in live cells.
  JOURNAL   BMC Cell Biol. 2011 Dec 2;12:52.
  PUBMED    22136066
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2011-12-2"; volume: "12"; pages: "52"
COMMENT     Sequence Label: pEGFP-MRLC1 T18D, S19D
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(584..787)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(613..633)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctacc atggtggcga ccggtcggtc gtctttatcc ttggcgccat gtttgaggat
       61 gcgggtgaac tccacgtagt tgaagttgcc tttcttatca atgggtgcct cccggtacat
      121 ctcgtccact tcctcatctg tgaagcggtc acccatggtg gtgagcagct cccggaggtg
      181 gtcctcatgg atgaaacctg aggcttcctc gtcgaagcag gcaaaggcgt tgcgaatcac
      241 atcctcgggg tccgtgccgt tcagcttctc cccaaacatg gtgaggaaca tggtgaagtt
      301 gatgggcccc ggggcctcgc tcatcatgcc ctccaggtat tcgtctgtgg ggttcttccc
      361 cagcgaggcc agcatgtcgt gcaggtcctc cttgtcaatg aagccatcac ggttctggtc
      421 aatcatgttg aaagcttcct taaactcctg gatctgggac tggtcaaaca ttgcgaagac
      481 attgtcgtcg gcccgctgtg gccgcttctt ggtggtcttg gctttggccc gcttgctgga
      541 catggtggcg gatccgctag cggatctgac ggttcactaa accagctctg cttatataga
      601 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      661 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      721 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
      781 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaa
//
