LOCUS       sequence-447573-        3027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase 
            (dsbC) constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Gent-EM7-Erv1p-DsbC
FEATURES             Location/Qualifiers
     source          1..3027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1009..1056
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     terminator      2347..2374
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2380..2470
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgt tgacaattaa
     1021 tcatcggcat agtatatcgg catagtataa tacgacgtgt aggaggataa tctatgaagg
     1081 caattgataa gatgaccgac aatcctcctc aggaaggctt gtccgggcgc aaaatcatct
     1141 atgatgagga cgggaagccg tgccgttcct gcaatacttt gctggacttc caatacgtca
     1201 cgggtaaaat ctcgaatgga ttgaagaatt tgtcttcaaa tggaaaactg gcagggactg
     1261 gggctcttac gggggaggca tcggaactta tgcctggttc ccgcacttat cgcaaagtag
     1321 atccacctga tgttgagcag cttggccgtt cttcgtggac cttgttgcac tctgtcgccg
     1381 cgtcgtatcc agctcagcct acggaccaac agaagggcga gatgaaacag ttcctgaaca
     1441 tcttctcgca catttaccct tgcaactggt gcgccaagga cttcgagaag tacattcgcg
     1501 agaatgcgcc gcaagtagaa tcccgcgaag aattgggacg ttggatgtgt gaagcacata
     1561 ataaggtgaa taagaagtta cgcaaaccaa agttcgactg taatttctgg gaaaaacgct
     1621 ggaaagacgg gtgggacgag taatgatcta gagattaaag aggagaatac tagatggatg
     1681 acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa aagcagcgat attcagcccg
     1741 cgcctgtagc tggcatgaag acagttctga ctaacagcgg cgtgttgtac atcaccgatg
     1801 atggtaaaca tatcattcag gggccaatgt atgacgttag tggcacggct ccggtcaatg
     1861 tcaccaataa gatgctgtta aagcagttga atgcgcttga aaaagagatg atcgtttata
     1921 aagcgccgca ggaaaaacac gtcatcaccg tgtttactga tattacctgt ggttactgcc
     1981 acaaactgca tgagcaaatg gcagactaca acgcgctggg gatcaccgtg cgttatcttg
     2041 ctttcccgcg ccaggggctg gacagcgatg cagagaaaga aatgaaagct atctggtgtg
     2101 cgaaagataa aaacaaagcg tttgatgatg tgatggcagg taaaagcgtc gcaccagcca
     2161 gttgcgacgt ggatattgcc gaccattacg cacttggcgt ccagcttggc gttagcggta
     2221 ctccggcagt tgtgctgagc aatggcacac ttgttccggg ttaccagccg ccgaaagaga
     2281 tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg taaataatga ggatccgagt
     2341 cacactggct caccttcggg tgggcctttc tgcgtttatt gatcggcacg taagaggttc
     2401 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     2461 ttcaggagct ggaaggaagt aaaaatgttg cgtagctcta acgatgtgac gcaacaaggt
     2521 tcgcgtccaa agacaaaatt gggaggcagt agcatgggga tcattcgcac ttgtcgcctg
     2581 gggccagacc aggtgaagtc aatgcgtgcg gctctggact tattcgggcg cgaatttgga
     2641 gatgtagcca cttactcaca gcaccaaccg gacagtgatt acttggggaa tttacttcgc
     2701 agtaaaactt ttatcgcttt ggccgctttc gaccaggagg ctgtagtagg tgcgttggca
     2761 gcctatgttc ttcctaaatt cgagcaaccg cgtagcgaaa tttacatcta tgatcttgca
     2821 gtctccggcg aacatcgccg tcaggggatc gccacagctt taatcaacct tttgaagcat
     2881 gaggctaatg cacttggagc gtacgtgatt tatgtgcagg ctgactacgg tgatgatcct
     2941 gcagtcgctc tgtacaccaa actgggtatc cgcgaggagg tcatgcactt tgatattgac
     3001 ccgtctacgg ctacctaata gaagctt
//
