LOCUS       sequence-447584-        9561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant protein for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, 
            Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Uoselis L, 
            Lazarou M, Hummer G, Hurley JH, Martens S
  TITLE     Reconstitution of BNIP3/NIX-mitophagy initiation reveals 
            hierarchical flexibility of the autophagy machinery.
  JOURNAL   Nat Cell Biol. 2025 Jul 25. doi: 10.1038/s41556-025-01712-y.
  PUBMED    40715440
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Jul 25. doi: 10.1038/s41556-025-01712-y."
COMMENT     Sequence Label: pBIG1a_GST-TEV-CK2alpha_CK2beta
FEATURES             Location/Qualifiers
     source          1..9561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             442..1233
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1361..1380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1754..1858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1859..1927,1928..2719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2077..2096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2890..3478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3379..3398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3632..3649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3783..4007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4074..4607)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4796..4801,4802..4818,4819..4824))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5220..5253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5299..5318)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5343..5391
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5486..5577
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5663..6316
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     6274..6296
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             6332..6352
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7664..7798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7954..8045
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8865..8999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  9314..9479
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc
      121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
      181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg
      241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
      361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
      421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg
     1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3001 aactggcttc agcagagcgc agataccaaa tactattctt ctagtgtagc cgtagttagg
     3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca
     4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4801 acgaaccgaa caggcttatg tcaactgggt ttgtgccttc atccgtttcc acggtgtgcg
     4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg
     5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg
     5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca
     5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa
     5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa
     5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca
     5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
     5581 ccggattatt cataccgtcc caccatcggg cgcggatccc ggtccgacat atgagcgcgc
     5641 ggaattcatg tcagccctgc agatgtcccc tatactaggt tattggaaaa ttaagggcct
     5701 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
     5761 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
     5821 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
     5881 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
     5941 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
     6001 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
     6061 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
     6121 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
     6181 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     6241 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     6301 cgaccatcct ccaaaagtcg acggctccgg tgaaaacctg tatttccagg gaggtggcat
     6361 gtcgggaccc gtgccaagca gggccagagt ttacacagat gttaatacac acagacctcg
     6421 agaatactgg gattacgagt cacatgtggt ggaatgggga aatcaagatg actaccagct
     6481 ggttcgaaaa ttaggccgag gtaaatacag tgaagtattt gaagccatca acatcacaaa
     6541 taatgaaaaa gttgttgtta aaattctcaa gccagtaaaa aagaagaaaa ttaagcgtga
     6601 aataaagatt ttggagaatt tgagaggagg tcccaacatc atcacactgg cagacattgt
     6661 aaaagaccct gtgtcacgaa cccccgcctt ggtttttgaa cacgtaaaca acacagactt
     6721 caagcaattg taccagacgt taacagacta tgatattcga ttttacatgt atgagattct
     6781 gaaggccctg gattattgtc acagcatggg aattatgcac agagatgtca agccccataa
     6841 tgtcatgatt gatcatgagc acagaaagct acgactaata gactggggtt tggctgagtt
     6901 ttatcatcct ggccaagaat ataatgtccg agttgcttcc cgatacttca aaggtcctga
     6961 gctacttgta gactatcaga tgtacgatta tagtttggat atgtggagtt tgggttgtat
     7021 gctggcaagt atgatctttc ggaaggagcc atttttccat ggacatgaca attatgatca
     7081 gttggtgagg atagccaagg ttctggggac agaagattta tatgactata ttgacaaata
     7141 caacattgaa ttagatccac gtttcaatga tatcttgggc agacactctc gaaagcgatg
     7201 ggaacgcttt gtccacagtg aaaatcagca ccttgtcagc cctgaggcct tggatttcct
     7261 ggacaaactg ctgcgatatg accaccagtc acggcttact gcaagagagg caatggagca
     7321 cccctatttc tacactgttg tgaaggacca ggctcgaatg ggttcatcta gcatgccagg
     7381 gggcagtacg cccgtcagca gcgccaatat gatgtcaggg atttcttcag tgccaacccc
     7441 ttcacccctt ggacctctgg caggctcacc agtgattgct gctgccaacc cccttgggat
     7501 gcctgttcca gctgccgctg gcgctcagca gtaaaagctt gtcgagaagt actagaggat
     7561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     7801 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     7861 ctgctgccac cgctgagcaa taactatcat aaccatttaa ataaactgga tactattgca
     7921 cgtttaaatc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     7981 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     8041 taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg agcagctcag
     8101 aggaggtgtc ctggatttcc tggttctgtg ggctccgtgg caatgaattc ttctgtgaag
     8161 tggatgaaga ctacatccag gacaaattta atcttactgg actcaatgag caggtccctc
     8221 actatcgaca agctctagac atgatcttgg acctggagcc tgatgaagaa ctggaagaca
     8281 accccaacca gagtgacctg attgagcagg cagccgagat gctttatgga ttgatccacg
     8341 cccgctacat ccttaccaac cgtggcatcg cccagatgtt ggaaaagtac cagcaaggag
     8401 actttggtta ctgtcctcgt gtgtactgtg agaaccagcc aatgcttccc attggccttt
     8461 cagacatccc aggtgaagcc atggtgaagc tctactgccc caagtgcatg gatgtgtaca
     8521 cacccaagtc atcaagacac catcacacgg atggcgccta cttcggcact ggtttccctc
     8581 acatgctctt catggtgcat cccgagtacc ggcccaagag acctgccaac cagtttgtgc
     8641 ccaggctcta cggtttcaag atccatccga tggcctacca gctgcagctc caagccgcca
     8701 gcaacttcaa gagcccagtc aagacgattc gctgaaagct tgtcgagaag tactagagga
     8761 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     8821 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     8881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     9001 gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg
     9061 gctgctgcca ccgctgagca ataactatca taaccattta aataaatcta tatctcaatc
     9121 ggggttacgt cgatacccca ccgagacccc attgggacca atacgcccgc gtttcttcct
     9181 tttccccacc ccaaccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     9241 cggcaagccc tgccatagcc actacgggtt aattaaaaac tatatctcaa tcggggttta
     9301 aacaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     9361 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     9421 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
     9481 cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa
     9541 accgtcatct tcggctactt t
//
