LOCUS       sequence-447620-       10401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral TMEM230(X121W) expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, 
            Walter JC, Harper JW
  TITLE     EndoMAP.v1 charts the structural landscape of human early endosome 
            complexes.
  JOURNAL   Nature. 2025 Jul;643(8070):252-261. doi: 10.1038/s41586-025-09059-y.
            Epub 2025 May 28.
  PUBMED    40437099
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09059-y"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "252-261"
COMMENT     Sequence Label: plenti-UBC-HA-TMEM230(X121W)
FEATURES             Location/Qualifiers
     source          1..10401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1069..1267
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2134..2367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2552..2596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2894..3011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3103..4314
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4151..4170
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4369..4395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4417..4441
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4827..4851)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4995..5494
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5030..5053)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5427..5446
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5512..6537
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5512..6537
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6568..6601)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6657..7245
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6710..6730)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7128..7139)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7248..7264)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7249..7265)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7454..7687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7759..7880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7796..7815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7850..7869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7920..8055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8075..8094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8076..8094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(8117..8475)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8157..8178)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8369..8388
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8502..8606
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8608..8965
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8608..8799
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8631..8651)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8816..8951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9000..9794
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9000..9794
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9054..9073)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9664..9683
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9982..10001)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10026..10073
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2041 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2101 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2161 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2221 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2281 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2341 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2401 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2461 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2521 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2581 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2641 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2701 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2761 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2821 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2881 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2941 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3001 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc
     3061 cgctctagcc ctagatttaa cccaacagaa ggctcgacgc gtggcctccg cgccgggttt
     3121 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     3181 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     3241 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     3301 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     3361 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     3421 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     3481 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     3541 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     3601 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     3661 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     3721 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     3781 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     3841 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     3901 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     3961 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     4021 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     4081 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     4141 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     4201 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     4261 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgctaga
     4321 ctgccggatc gaattgtcga cgaattcgga tccagatctg ccaccatgta cccatacgat
     4381 gttccagatt acgctggcgg agctcgaggt tatcaaacaa gtttgtacaa aaaagcaggc
     4441 tccatgatgc cgtcccgtac caacctggct actggaatcc ccagtagtaa agtgaaatat
     4501 tcaaggctct ccagcacaga cgatggctac attgaccttc agtttaagaa aacccctcct
     4561 aagatccctt ataaggccat cgcacttgcc actgtgctgt ttttgattgg cgcctttctc
     4621 attattatag gctccctcct gctgtcaggc tacatcagca aagggggggc agaccgggcc
     4681 gttccagtgc tgatcattgg cattctggtg ttcctacccg gattttacca cctgcgcatc
     4741 gcttactatg catccaaagg ctaccgtggt tactcctatg atgacattcc agactttgat
     4801 gactggcacc caccccatag ctgatgaccc agctttcttg tacaaagtgg ttcgataacg
     4861 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     4921 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggccgcgtt
     4981 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5041 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     5101 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     5161 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     5221 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     5281 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     5341 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     5401 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     5461 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atcccgccac catgaaaaag
     5521 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     5581 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     5641 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     5701 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     5761 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5821 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     5881 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5941 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     6001 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     6061 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     6121 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     6181 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     6241 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     6301 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     6361 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     6421 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     6481 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagtgt
     6541 acaagtagga attcgtcgag ggacctaata acttcgtata gcatacatta tacgaagtta
     6601 tacatgttta agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc
     6661 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6721 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6781 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6841 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6901 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6961 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7021 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7081 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7141 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7201 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7261 ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7321 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7381 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7441 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7681 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7741 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7861 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8041 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attagcatgt
     8101 gagcaaaagg ccagcataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8161 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     8221 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8281 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8341 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8401 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8461 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8581 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     8641 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8701 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8761 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8821 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8881 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8941 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     9001 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     9061 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     9121 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     9181 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     9241 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     9301 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     9361 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     9421 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     9481 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     9541 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9601 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9661 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9721 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9781 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     9841 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     9901 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     9961 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
    10021 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
    10081 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
    10141 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
    10201 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
    10261 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10381 agaaaagatc aaaggatctt c
//
