LOCUS       sequence-447623-        8634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino 
            acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(154..945,946..1014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     777..796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1015..1119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1198..1217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1403..1688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          1990..2217
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2229..2238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2235..6338
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFKRPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAHWLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKMYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6393..6413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6426..6491
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6510..6566
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6567..7277
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             7286..7303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7326..7343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7332..7556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7627..7643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7627..7643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7640..7662)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7651..7667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7675..7681,7682..7699,7700..7705))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7720..7741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7858..7875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8029..8617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8109..8128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
       61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
     1201 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     1261 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     1321 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     1381 tacgcgtcta gatcagggta cccgttacat aacttacggt aaatggcccg cctggctgac
     1441 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
     1501 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1561 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
     1621 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1681 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1741 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1801 gggggggggg ggcgcgcccg gggggggggg gggggggggg gggggggggg ggggcggggc
     1861 gggggggggg ggggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1921 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1981 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     2041 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2101 ccttctcctc cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg atggttggtt
     2161 ggtggggtat taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt
     2221 ggaccggcgc caccatggac aagaagtaca gcatcggcct ggacatcggc accaactctg
     2281 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
     2341 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
     2401 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
     2461 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
     2521 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
     2581 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
     2641 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
     2701 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
     2761 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
     2821 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
     2881 tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg
     2941 gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact
     3001 tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg
     3061 acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc
     3121 tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc
     3181 ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga
     3241 aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca
     3301 agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca
     3361 tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag
     3421 aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc
     3481 tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca
     3541 accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg
     3601 ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct
     3661 ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga
     3721 ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
     3781 agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa
     3841 agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca
     3901 accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg
     3961 actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg
     4021 atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc
     4081 tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc
     4141 tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat
     4201 acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg
     4261 gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc
     4321 tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc
     4381 agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg
     4441 gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc
     4501 ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga
     4561 acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc
     4621 tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc
     4681 tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact
     4741 acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg
     4801 tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg
     4861 tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa
     4921 agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct
     4981 tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg
     5041 actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga
     5101 tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc
     5161 gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg
     5221 ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt
     5281 acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt
     5341 acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg
     5401 agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata
     5461 agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga
     5521 aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca
     5581 gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcaaga
     5641 gacccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga
     5701 aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga
     5761 agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca
     5821 tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct
     5881 ctgcccactg gctgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc
     5941 tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac
     6001 agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt
     6061 tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca
     6121 agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga
     6181 ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac cggaagatgt
     6241 acagaagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt
     6301 acgagacacg gatcgacctg tctcagctgg gaggcgacgg atccggcgga ggcggaagcg
     6361 ggaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa gtctcgagcg
     6421 gaggcgacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
     6481 acgatgacaa gggaggcggt ggaagcggcg caacaaactt ctctctgctg aaacaagccg
     6541 gagatgtcga agagaatcct ggaccgatgg tgtctaaggg cgaagagctg attaaggaga
     6601 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
     6661 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     6721 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
     6781 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
     6841 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     6901 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
     6961 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
     7021 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
     7081 gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca
     7141 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
     7201 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
     7261 tggggcacaa acttaattga ccggtcatca tcaccatcac cattgagttt aaacccgctg
     7321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7561 tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta
     7621 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7681 acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt
     7741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7801 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7861 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7921 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7981 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8041 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8101 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8161 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8221 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8281 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8341 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8401 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8461 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8521 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8581 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tctt
//
