LOCUS       sequence-447626-        8639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino 
            acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)
FEATURES             Location/Qualifiers
     source          1..8639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(180..971,972..1040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     803..822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1041..1145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1224..1243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1429..1714
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          2021..2248
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2260..2269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2266..6369
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFKRPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASATMLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6424..6444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6457..6522
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6541..6597
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6598..7308
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             7317..7334
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7357..7374)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7363..7587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7658..7674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7658..7674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7671..7693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7682..7698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7706..7712,7713..7730,7731..7736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7751..7772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7889..7906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8060..8639,1..9))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8140..8159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      181 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      241 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      301 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      361 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      421 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      481 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      541 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      601 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      661 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      721 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      781 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      841 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      901 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      961 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1021 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1081 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1141 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc
     1201 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     1261 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     1321 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     1381 ctgcttcgcg atgtacgggc cagatatacg cgtctagatc agggtacccg ttacataact
     1441 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt
     1501 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1561 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1621 taaatggccc gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca
     1681 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1741 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1801 attttgtgca gcgatggggg cggggggggg gggggggggc gccccaggcg gggggggggg
     1861 gggggggggg gggggggggg gggggggggg gggggggggg ggcggggcga ggcggagagg
     1921 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1981 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     2041 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     2101 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag
     2161 caagaggtaa gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga
     2221 gcacctgcct gaaatcactt tttttcaggt tggaccggcg ccaccatgga caagaagtac
     2281 agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     2341 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     2401 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     2461 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     2521 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     2581 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     2641 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     2701 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     2761 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     2821 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     2881 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     2941 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     3001 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     3061 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     3121 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     3181 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     3241 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     3301 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     3361 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     3421 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     3481 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     3541 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     3601 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     3661 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     3721 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     3781 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     3841 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     3901 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     3961 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     4021 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     4081 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     4141 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     4201 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     4261 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     4321 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     4381 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     4441 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     4501 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     4561 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     4621 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     4681 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     4741 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     4801 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     4861 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     4921 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     4981 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     5041 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     5101 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     5161 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     5221 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     5281 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     5341 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     5401 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     5461 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     5521 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     5581 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     5641 tgggacccta agaagtacgg cggcttcaag agacccaccg tggcctattc tgtgctggtg
     5701 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     5761 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     5821 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     5881 ctggaaaacg gccggaagag aatgctggcc tctgccacca tgctgcagaa gggaaacgaa
     5941 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     6001 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     6061 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     6121 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     6181 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     6241 tactttgaca ccaccatcga ccggaagcag tacagaagca ccaaagaggt gctggacgcc
     6301 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     6361 ggaggcgacg gatccggcgg aggcggaagc gggaaaagaa ccgccgacgg cagcgaattc
     6421 gagcccaaga agaagaggaa agtctcgagc ggaggcgact acaaagacca tgacggtgat
     6481 tataaagatc atgacatcga ttacaaggat gacgatgaca agggaggcgg tggaagcggc
     6541 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     6601 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
     6661 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     6721 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     6781 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     6841 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     6901 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     6961 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     7021 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     7081 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca
     7141 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     7201 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     7261 gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaattg accggtcatc
     7321 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7381 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7441 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7501 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7561 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     7621 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7681 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7741 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7801 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7861 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8041 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8461 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaa
//
