LOCUS       sequence-447631-        8614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino 
            acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)
FEATURES             Location/Qualifiers
     source          1..8614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1420..1705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1707..1986
                     /label=chicken beta-actin promoter
     intron          1987..2214
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2226..2235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2232..6335
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFMRPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARWLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKYYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6390..6410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6423..6488
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6507..6563
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6564..7274
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             7283..7300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7323..7340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7329..7553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7624..7640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7624..7640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7637..7659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7648..7664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7672..7678,7679..7696,7697..7702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7717..7738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7855..7872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8026..8614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8106..8125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgtctagat cagggtaccc gttacataac ttacggtaaa
     1441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
     1501 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1561 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1621 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1681 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     1741 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     1801 agcgatgggg gcgggggggg gggggggggg cgcgcgccgg gggggggggg gsggggsgrg
     1861 gggsggggsg gggsgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     1921 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     1981 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     2041 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     2101 tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg gttggttggt
     2161 ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga
     2221 ccggcgccac catggacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
     2281 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     2341 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     2401 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     2461 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     2521 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     2581 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     2641 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     2701 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     2761 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     2821 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     2881 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     2941 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     3001 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     3061 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     3121 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     3181 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     3241 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     3301 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     3361 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     3421 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     3481 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     3541 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     3601 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     3661 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     3721 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     3781 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     3841 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     3901 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     3961 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     4021 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     4081 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     4141 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     4201 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     4261 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     4321 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     4381 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     4441 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     4501 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     4561 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     4621 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     4681 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     4741 atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     4801 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     4861 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     4921 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     4981 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     5041 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     5101 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     5161 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     5221 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     5281 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     5341 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     5401 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     5461 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     5521 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     5581 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcatgagac
     5641 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     5701 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     5761 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     5821 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     5881 ccagatggct gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     5941 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     6001 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     6061 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     6121 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     6181 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagtactaca
     6241 gaagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     6301 agacacggat cgacctgtct cagctgggag gcgacggatc cggcggaggc ggaagcggga
     6361 aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc tcgagcggag
     6421 gcgactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     6481 atgacaaggg aggcggtgga agcggcgcaa caaacttctc tctgctgaaa caagccggag
     6541 atgtcgaaga gaatcctgga ccgatggtgt ctaagggcga agagctgatt aaggagaaca
     6601 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     6661 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     6721 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     6781 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     6841 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     6901 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     6961 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     7021 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     7081 atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga
     7141 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     7201 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     7261 ggcacaaact taattgaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     7321 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7381 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7441 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7501 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7561 ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc
     7621 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7681 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     7741 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8461 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8581 gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
