LOCUS       sequence-447644-       10587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV and pT7 Human expression plasmid for 
            PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCMV-T7-PEmax-P2A-EGFP (LM1589)
FEATURES             Location/Qualifiers
     source          1..10587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        434..637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     588..608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     679..698
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             768..4868
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4926..4946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7047..7067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7077..7103
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7122..7178
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7179..7181,7182..7184,7185..7898)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7179..7181,7182..7184,7185..7895)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7485..7504)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7832..7853
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7904..7921
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7944..7961)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7950..8174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8245..8261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8245..8261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8258..8280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8269..8285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8293..8299,8300..8317,8318..8323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8338..8359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8476..8493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8647..9235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8727..8746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9406..10197,10198..10266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10029..10048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10267..10371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10450..10469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      661 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga
      721 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
      781 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      841 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      901 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      961 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1021 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1081 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1141 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1201 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1261 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1321 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1381 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1441 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1501 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1561 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1621 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1681 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1741 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1801 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1861 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1921 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg
     1981 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2041 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2101 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2161 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2221 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2281 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2341 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2401 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2461 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2521 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2581 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2641 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2701 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2761 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2821 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2881 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2941 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3001 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3061 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3121 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3181 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3241 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3301 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3361 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3421 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3481 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3541 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3601 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3661 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3721 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3781 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3841 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3901 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     3961 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4021 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4081 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4141 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4201 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4261 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4321 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4381 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4441 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4501 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4561 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4621 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4681 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4741 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4801 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4861 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccgacggc tctgaattcg
     4921 agagccctaa gaagaaaaga aaggtgagcg gaggctctag cggcggaagc accctgaaca
     4981 ttgaagacga gtatagactg catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca
     5041 cctggctgtc cgactttccc caggcctggg ccgagacagg aggaatgggc ctggccgtgc
     5101 ggcaggcacc cctgatcatc cctctgaagg ccacctctac acccgtgagc atcaagcagt
     5161 accctatgtc tcaggaggcc agactgggca tcaagcctca catccagagg ctgctggacc
     5221 agggcatcct ggtgccatgc cagagcccct ggaacacacc actgctgccc gtgaagaagc
     5281 caggcaccaa tgactataga cccgtgcagg atctgagaga ggtgaacaag agggtggagg
     5341 atatccaccc caccgtgccc aacccttaca atctgctgtc cggcctgccc ccttctcacc
     5401 agtggtatac agtgctggac ctgaaggatg ccttcttttg tctgagactg caccctacca
     5461 gccagccact gttcgccttt gagtggaggg accctgagat gggcatctct ggccagctga
     5521 cctggacacg cctgcctcag ggcttcaaga atagcccaac actgtttaac gaggccctgc
     5581 accgcgacct ggcagatttc cggatccagc acccagatct gatcctgctg cagtacgtgg
     5641 acgatctgct gctggccgcc accagcgagc tggattgcca gcagggaaca cgcgccctgc
     5701 tgcagaccct gggaaacctg ggatataggg catccgccaa gaaggcccag atctgtcaga
     5761 agcaggtgaa gtacctgggc tatctgctga aggagggcca gagatggctg acagaggcca
     5821 ggaaggagac agtgatgggc cagccaacac ccaagacccc aagacagctg agggagttcc
     5881 tgggcaaagc aggattttgc aggctgttca tcccaggatt cgcagagatg gcagcacctc
     5941 tgtacccact gaccaagccg ggcaccctgt ttaattgggg ccctgaccag cagaaggcct
     6001 atcaggagat caagcaggcc ctgctgacag caccagccct gggcctgcca gacctgacca
     6061 agcctttcga gctgtttgtg gatgagaagc agggctacgc caagggcgtg ctgacccaga
     6121 agctgggacc atggagacgg cccgtggcct atctgtccaa gaagctggac ccagtggcag
     6181 caggatggcc accatgcctg aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg
     6241 gcaagctgac catgggacag ccactggtca tcctggcacc acacgcagtg gaggccctgg
     6301 tgaagcagcc tccagatcgc tggctgtcta acgcccggat gacacactac caggccctgc
     6361 tgctggacac cgatcgcgtg cagtttggcc ctgtggtggc cctgaatcca gccaccctgc
     6421 tgcctctgcc agaggagggc ctgcagcaca actgtctgga catcctggca gaggcacacg
     6481 gaacaaggcc agacctgacc gatcagcccc tgcctgacgc cgatcacaca tggtataccg
     6541 atggaagctc cctgctgcag gagggccaga ggaaggcagg agcagcagtg accacagaga
     6601 cagaagtgat ctgggccaag gccctgccag caggcacatc cgcccagcgg gccgagctga
     6661 tcgccctgac ccaggccctg aagatggccg agggcaagaa gctgaacgtg tacacagact
     6721 ccagatatgc cttcgccacc gcacacatcc acggagagat ctacaggcgc cggggctggc
     6781 tgacctctga gggcaaggag atcaagaaca aggatgagat cctggccctg ctgaaggccc
     6841 tgtttctgcc caagcggctg agcatcatcc actgtcctgg acaccagaag ggacactccg
     6901 ccgaggcaag gggcaatcgg atggccgacc aggccgccag aaaggctgct attactgaaa
     6961 ctcccgacac ttccactctg ctgattgaaa actcctcccc ttctggcggc tcaaaaagaa
     7021 ccgccgacgg cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg
     7081 ccgctaagag agtgaagctg gacggaggcg gtggaagcgg cgcaacaaac ttctctctgc
     7141 tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggtgagcaag ggcgaggagc
     7201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7381 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7441 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     7621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     7681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     7741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     7801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     7861 ccgggatcac tctcggcatg gacgagctgt acaagtaacc ggtcatcatc accatcacca
     7921 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7981 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8041 ataaaatgag aaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8101 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8161 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc
     8221 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8281 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg
     8341 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8401 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9061 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9241 tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat
     9301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10381 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
    10441 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    10501 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    10561 gaccgacaat tgcatgaaga atctgct
//
