LOCUS       sequence-447650-        8451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KRRCKV 
            amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCMV-T7-SpCas9(KRRCKV)-P2A-EGFP (RAS3014)
FEATURES             Location/Qualifiers
     source          1..8451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(72..89)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(243..831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(323..342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1002..1793,1794..1862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1625..1644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1863..1967)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2046..2065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2237..2616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2617..2820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2771..2791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2862..2881
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2862..2880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2888..2897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2894..6997
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFKRPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARCLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYVSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7052..7072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7085..7150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7169..7225
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7226..7228,7229..7231,7232..7945)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7226..7228,7229..7231,7232..7942)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7532..7551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7879..7900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7951..7968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7991..8008)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7997..8221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8292..8308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8292..8308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8305..8327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8316..8332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8340..8346,8347..8364,8365..8370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8385..8406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
       61 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1981 aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact
     2041 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     2101 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     2161 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     2221 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     2281 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2821 ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg
     2881 gagagccgcc accatggaca agaagtacag catcggcctg gacatcggca ccaactctgt
     2941 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     3001 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     3061 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     3121 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     3181 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     3241 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     3301 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     3361 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     3421 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     3481 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     3541 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     3601 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     3661 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     3721 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     3781 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     3841 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     3901 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     3961 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     4021 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     4081 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     4141 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     4201 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     4261 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     4321 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     4381 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     4441 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     4501 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     4561 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     4621 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     4681 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     4741 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     4801 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     4861 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     4921 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     4981 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     5041 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     5101 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     5161 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     5221 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     5281 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     5341 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     5401 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     5461 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     5521 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     5581 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     5641 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     5701 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     5761 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     5821 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     5881 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     5941 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     6001 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     6061 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     6121 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     6181 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     6241 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcaagag
     6301 acccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     6361 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     6421 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     6481 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     6541 tgccagatgc ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     6601 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     6661 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     6721 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     6781 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     6841 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaagta
     6901 cgtgagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     6961 cgagacacgg atcgacctgt ctcagctggg aggcgacgga tccggcggag gcggaagcgg
     7021 gaaaagaacc gccgacggca gcgaattcga gcccaagaag aagaggaaag tctcgagcgg
     7081 aggcgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     7141 cgatgacaag ggaggcggtg gaagcggcgc aacaaacttc tctctgctga aacaagccgg
     7201 agatgtcgaa gagaatcctg gaccgatggt gagcaagggc gaggagctgt tcaccggggt
     7261 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7321 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7381 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     7441 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7501 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7561 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7621 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     7681 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     7741 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7801 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     7861 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     7921 cggcatggac gagctgtaca agtgaccggt catcatcacc atcaccattg agtttaaacc
     7981 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8041 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8101 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8161 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     8221 gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg
     8281 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8341 aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc
     8401 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc g
//
