LOCUS       sequence-447661-        8451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK 
            amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)
FEATURES             Location/Qualifiers
     source          1..8451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1020..1811,1812..1880))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1643..1662
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1881..1985)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2064..2083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2255..2634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2635..2838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2789..2809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2880..2899
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2880..2898
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2912..7015
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARYLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKEYKSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7070..7090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7103..7168
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7187..7243
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7244..7246,7247..7249,7250..7963)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7244..7246,7247..7249,7250..7960)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7550..7569)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7897..7918
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7969..7986
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8009..8026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8015..8239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8310..8326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8310..8326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8323..8345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8334..8350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8358..8364,8365..8382,8383..8388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8403..8424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
       61 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1981 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc
     2041 gatcccctag ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     2101 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     2161 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     2221 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     2281 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     2341 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2401 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2461 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2521 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2581 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2641 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2701 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2761 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2821 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct
     2881 aatacgactc actataggga gagccgccac catggacaag aagtacagca tcggcctgga
     2941 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
     3001 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
     3061 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
     3121 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
     3181 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
     3241 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     3301 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     3361 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     3421 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     3481 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     3541 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     3601 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     3661 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     3721 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     3781 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     3841 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     3901 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     3961 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     4021 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     4081 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     4141 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     4201 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     4261 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     4321 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     4381 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     4441 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     4501 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     4561 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     4621 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     4681 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     4741 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     4801 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     4861 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     4921 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     4981 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     5041 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     5101 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     5161 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     5221 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     5281 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     5341 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     5401 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     5461 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     5521 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     5581 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     5641 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     5701 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     5761 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     5821 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     5881 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     5941 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     6001 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     6061 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     6121 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     6181 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     6241 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     6301 gtacggcggc ttcctgtggc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     6361 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     6421 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     6481 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     6541 gaagagaatg ctggcctctg ccagatacct gcagaaggga aacgaactgg ccctgccctc
     6601 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     6661 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     6721 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     6781 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     6841 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     6901 catcgaccgg aaggagtaca agagcaccaa agaggtgctg gacgccaccc tgatccacca
     6961 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacggatc
     7021 cggcggaggc ggaagcggga aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa
     7081 gaggaaagtc tcgagcggag gcgactacaa agaccatgac ggtgattata aagatcatga
     7141 catcgattac aaggatgacg atgacaaggg aggcggtgga agcggcgcaa caaacttctc
     7201 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggtga gcaagggcga
     7261 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7321 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     7381 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     7441 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     7501 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     7561 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7621 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     7681 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     7741 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7801 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7861 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     7921 cgccgccggg atcactctcg gcatggacga gctgtacaag tgaccggtca tcatcaccat
     7981 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8041 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8101 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8161 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8221 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     8281 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8341 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc
     8401 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt c
//
