LOCUS       sequence-447666-        8451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCKS 
            amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term 
            BPNLS-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Silverstein RA, Kim N, Kroell AS, Walton RT, Delano J, Butcher RM, 
            Pacesa M, Smith BK, Christie KA, Ha LL, Meis RJ, Clark AB, Spinner 
            AD, Lazzarotto CR, Li Y, Matsubara A, Urbina EO, Dahl GA, Correia 
            BE, Marks DS, Tsai SQ, Pinello L, De Ravin SS, Liu Q, Kleinstiver BP
  TITLE     Custom CRISPR-Cas9 PAM variants via scalable engineering and machine
            learning.
  JOURNAL   Nature. 2025 Apr 22. doi: 10.1038/s41586-025-09021-y.
  PUBMED    40262634
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Apr 22. doi: 10.1038/s41586-025-09021-y."
COMMENT     Sequence Label: pCMV-T7-SpCas9(MKRCKS)-P2A-EGFP (AHK140)
FEATURES             Location/Qualifiers
     source          1..8451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(76..93)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(247..835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(327..346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1006..1797,1798..1866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1629..1648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1867..1971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2050..2069)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2241..2620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2621..2824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2775..2795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2866..2885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2866..2884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2892..2901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2898..7001
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFMKPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARCLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKKYSSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7056..7076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7089..7154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7173..7229
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7230..7232,7233..7235,7236..7949)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7230..7232,7233..7235,7236..7946)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7536..7555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7883..7904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7955..7972
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7995..8012)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8001..8225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8296..8312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8296..8312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8309..8331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8320..8336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8344..8350,8351..8368,8369..8374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8389..8410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
       61 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1141 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1321 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1621 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1981 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
     2041 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     2101 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     2161 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     2221 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     2281 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2341 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2401 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2461 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2521 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2581 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2641 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2701 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2761 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2821 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
     2881 tagggagagc cgccaccatg gacaagaagt acagcatcgg cctggacatc ggcaccaact
     2941 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     3001 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     3061 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     3121 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     3181 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     3241 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     3301 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     3361 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     3421 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     3481 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     3541 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     3601 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     3661 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     3721 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     3781 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     3841 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     3901 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     3961 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     4021 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     4081 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     4141 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     4201 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     4261 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     4321 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     4381 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     4441 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     4501 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     4561 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     4621 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     4681 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     4741 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     4801 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     4861 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     4921 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     4981 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     5041 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     5101 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     5161 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     5221 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     5281 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     5341 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     5401 actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     5461 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     5521 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     5581 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     5641 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     5701 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     5761 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     5821 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     5881 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     5941 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     6001 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     6061 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     6121 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     6181 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     6241 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttca
     6301 tgaagcccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     6361 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     6421 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     6481 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     6541 cctctgccag atgcctgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     6601 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     6661 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     6721 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     6781 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     6841 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     6901 agtacagcag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     6961 tgtacgagac acggatcgac ctgtctcagc tgggaggcga cggatccggc ggaggcggaa
     7021 gcgggaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg aaagtctcga
     7081 gcggaggcga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
     7141 atgacgatga caagggaggc ggtggaagcg gcgcaacaaa cttctctctg ctgaaacaag
     7201 ccggagatgt cgaagagaat cctggaccga tggtgagcaa gggcgaggag ctgttcaccg
     7261 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     7321 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     7381 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     7441 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     7501 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     7561 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7621 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7681 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     7741 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7801 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     7861 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7921 ctctcggcat ggacgagctg tacaagtgac cggtcatcat caccatcacc attgagttta
     7981 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8041 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8101 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8161 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8221 tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag
     8281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8341 acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta
     8401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc t
//
