LOCUS       sequence-44772-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F2-GCT
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..35
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             36..101
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 actgccccat ggctccaaag aagaagcgta aggtagacta caaagaccat gacggtgatt
       61 ataaagatca tgacatcgat tacaaggatg acgatgacaa gggtacccgc ccatatgctt
      121 gccctgtcga gtcctgtgac cgccgcttca gtcagtctgg tgacctgacc cgtcacatcc
      181 ggattcacac aggccagaag cccttccagt gtcgcatctg catgaggaac ttcagccacc
      241 ggcagagcct gacacggcac atccgcaccc acacaggcga gaagcctttt gcatgcgaca
      301 tctgcggcag gaagttcgcg cgcagcgaca acctctctga gcacaccaag atccacacag
      361 gtggatccca actagtcaaa agtgaactgg aggagaagaa atctgaactt cgtcataaat
      421 tgaaatatgt gcctcatgaa tatattgaat taattgaaat tgccagaaat tccactcagg
      481 atagaattct tgaaatgaag gtaatggaat tttttatgaa agtttatgga tatagaggta
      541 aacatttggg tggatcaagg aaaccggacg gagcaattta tactgtcgga tctcctattg
      601 attacggtgt gatcgtggat actaaagctt atagcggagg ttataatctg ccaattggcc
      661 aagcagatga aatgcaagac tatgtcgaag aaaatcaaac acgaaacaaa catatcaacc
      721 ctaatgaatg gtggaaagtc tatccatctt ctgtaacgga atttaagttt ttatttgtga
      781 gtggtcactt taaaggaaac tacaaagctc agcttacacg attaaatcat atcactaatt
      841 gtaatggagc tgttcttagt gtagaagagc ttttaattgg tggagaaatg a
//
