LOCUS       sequence-447723-        9381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for stable transformation of Geminino 1.0-eGFP flanked by two
            MAR10 insulators and next to a Hygromycin resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
FEATURES             Location/Qualifiers
     source          1..9381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(229..481)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(505..1530)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(505..1530)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1565..1744)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(2924..2926,2927..2929,2930..3640)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2924..2926,2927..2929,2930..3640)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2969..2990)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3230..3249)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3577..3598
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4657..5001
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4945..4965
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(6588..7379)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(7601..8263)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      8264..9035
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    9255..9279
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    9306..9330
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgcg agtcggtccc attattgaag catttatcag ggttattgtc tcatgagcgg
       61 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      121 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      181 gcgtatcacg aggccctttc gtcttcaaga attagcttgg caattcccga tctagtaaca
      241 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
      301 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
      361 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatcg
      421 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
      481 cgggatcgaa gatccattcc aagctcattc ctttgccctc ggacgagtgc tggggcgtcg
      541 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
      601 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
      661 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
      721 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
      781 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
      841 ggcgacctcg tattgggaat ccccgaacat tgcctcgctc cagtcaatga ccgctgttat
      901 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
      961 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     1021 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     1081 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     1141 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     1201 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     1261 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
     1321 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     1381 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     1441 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
     1501 agacgtcgcg gtgagttcag gctttttcat tgcctcgatc gagttgagag tgaatatgag
     1561 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
     1621 tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
     1681 tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt caacgttgcg
     1741 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataccgt
     1801 gactccctta attctcatgt atctccgtca ggagtggcta ttgttgttat catcaaaatc
     1861 tatattgata ctattatact cgatatgtta tagactttat taaaaaaaac tttgtttgtt
     1921 tgcagaaagt ggatttttaa tcaaatttaa ttgtgatgat tacaatgtca attggttatg
     1981 caagatatta attaatatgt ttaagatttt tcacttttaa taagtaaaat aaaatacgtc
     2041 aaaaaaattt cctaaagata atgccaaaaa ggttaaatta tagaaattaa actaaaatat
     2101 attaaaagtt gacttaaaaa tgcaatgttg accaccgacc taggggaaaa caaatttacc
     2161 gtatctggga tagaatctct tctgtatcat ctcagataaa gattactaca taaacacact
     2221 ttacagtttt acgacgccca aaattcaatt cccggagatt aaaaacccta aaacaaacac
     2281 tggcgattcg attttccctc ccaattcaaa tcatctctta atcttcttct tctcactatc
     2341 ctctcaaatt cgccgccgat cttccatcaa ttttgatttc tcgcaccgaa atttcgactt
     2401 ttcatctcct atcgacgaaa ccctaatttt tcacactcca gaaatcgaac tttgacgacg
     2461 atgttaatgg ctaaaccccg ctgtcaggag tagcagaagg catgttgttg tgactccgag
     2521 gggttgcctc aaactctatc ttataaccgg cgtggaggca tggaggcagg ggtattttgg
     2581 tcattttaat agatagtgga aaatgacgtg gaatttactt aaagacgaag tctttgcgac
     2641 aagggggggc ccacgccgaa tttaatatta ccggcgtggc ccccccttat cgcgagtgct
     2701 ttagcacgag cggtccagat ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt
     2761 tcacactata aaagcatata cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt
     2821 atttccgttg gatacgaatt attcgtacga ccctcctaat taacctgact caaaatctac
     2881 cctcataatt gttgtttgat attggtcttg tattttgcag gtaatggtga gcaagggcga
     2941 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3001 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3061 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3121 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3181 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3241 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3301 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3361 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3421 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3481 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3541 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3601 cgccgccggg atcactctcg gcatggacga gctgtacaag gacgagctgt aagcttcggc
     3661 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
     3721 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
     3781 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
     3841 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctataaaatg attattttat
     3901 gaatatattt cattgtgcaa gtagatagaa attacatatg ttacataaca cacgaaataa
     3961 acaaaaaaag acaatccaaa aacaaacacc ccaaaaaaaa taatcacttt agataaactc
     4021 gtatgaggag aggcacgttc aggtaactag agccaagctg atctcctttg ccccggagat
     4081 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
     4141 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
     4201 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
     4261 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
     4321 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
     4381 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
     4441 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
     4501 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
     4561 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
     4621 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     4681 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     4741 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     4801 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     4861 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     4921 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     4981 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     5041 acaaacaaca ttacaattta ctattctagt cgaagaaagg taagccatct tcctgcttat
     5101 ttttataatg aacatagaaa taggaagttg tgcagagaaa cgctgtcagg aggtgccaag
     5161 cttgcatgcc tgcaggtagc agaaggcatg ttgttgtgac tccgaggggt tgcctcaaac
     5221 tctatcttat aaccggcgtg gaggcatgga ggcaggggta ttttggtcat tttaatagat
     5281 agtggaaaat gacgtggaat ttacttaaag acgaagtctt tgcgacaagg gggggcccac
     5341 gccgaattta atattaccgg cgtggccccc ccttatcgcg agtgctttag cacgagcggt
     5401 ccagatttaa agtagaaaat ttcccgccca ctagggttaa aggtgttcac actataaaag
     5461 catatacgat gtgatggtat ttgatggagc gtatattgta tcaggtattt ccgttggata
     5521 cgaattattc gtacgaccct ccgctgtcag gagtggctat tgttgttatc atcaaaatct
     5581 atattgatac tattatactc gatatgttat agactttatt aaaaaaaact ttgtttgttt
     5641 gcagaaagtg gatttttaat caaatttaat tgtgatgatt acaatgtcaa ttggttatgc
     5701 aagatattaa ttaatatgtt taagattttt cacttttaat aagtaaaata aaatacgtca
     5761 aaaaaatttc ctaaagataa tgccaaaaag gttaaattat agaaattaaa ctaaaatata
     5821 ttaaaagttg acttaaaaat gcaatgttga ccaccgacct aggggaaaac aaatttaccg
     5881 tatctgggat agaatctctt ctgtatcatc tcagataaag attactacat aaacacactt
     5941 tacagtttta cgacgcccaa aattcaattc ccggagatta aaaaccctaa aacaaacact
     6001 ggcgattcga ttttccctcc caattcaaat catctcttaa tcttcttctt ctcactatcc
     6061 tctcaaattc gccgccgatc ttccatcaat tttgatttct cgcaccgaaa tttcgacttt
     6121 tcatctccta tcgacgaaac cctaattttt cacactccag aaatcgaact ttgacgacga
     6181 tgttaatggc taaaccccgc tgtcaggagt ctcaatggta actttactct ttatttaacc
     6241 atacattttt ttttattttt ttcactttgt tcttcatcca ctattgttct ttgttcatct
     6301 tgaacaaaag ctccctcctt ctttgttctt catccaccat tgttcttcat caatcatttc
     6361 gctgtcagga gaccggacaa ggatgcgagc tagcctgcag gaattgttga ttttgtgatg
     6421 actgatggca ggatatatgc ggttgtaatt catttttatt gtctaaattt ctgtatttgt
     6481 ttgtttgttc ggttgtaaat ttttttggaa gaacaagaaa agaaaaaaca cccgttaggg
     6541 tgtttttagt tagtgtggcg cgccgacttg cgacatgcgg tcctttgtta tttgccgact
     6601 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     6661 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     6721 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     6781 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     6841 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     6901 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     6961 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     7021 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     7081 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     7141 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     7201 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     7261 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     7321 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg
     7381 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
     7441 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     7501 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     7561 cgttcggtca aggttcgatc ggattggcgg ttatgcggtt ctaccggcgc ggcagcgtta
     7621 cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca tcgggcatcg
     7681 gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg
     7741 gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg gtcggtagtt
     7801 gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt
     7861 cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg cgcaactggt
     7921 cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg gtgccggggc
     7981 cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact gcgtattgga
     8041 ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc accacggcgt
     8101 tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg
     8161 caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct
     8221 tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc tccatgcggt
     8281 aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg gcagcgcgac
     8341 aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa cctacgcaat
     8401 gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt aagttgttga
     8461 tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa gaagggcacc
     8521 cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag tggcccctcc
     8581 ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct gcccccttgg
     8641 aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc gcccttcgac
     8701 tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg
     8761 tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca
     8821 ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc
     8881 aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc
     8941 cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc
     9001 cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac
     9061 gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg
     9121 ggccatcatc agttcggtgg tcttccgacg aacaataagg ccgcaaatcg cggccttttt
     9181 tattgataac aaaaccggct cagttctgcg tagaaaccaa catgcaagct ccaccgggtg
     9241 caaagcggca gcggcggcag gatatattca attgtaaatg gcttcatgtc cgggaaatct
     9301 actggtggca ggatatattg tggtgtaaac aatggagaaa aagattaatt aagtactagt
     9361 agcctggtcg aaacggtctc a
//
