LOCUS       sequence-447728-        3070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OCS terminator flanked by two att sites of PhiC31 integrase located 
            at position 112 of the SlDFR minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
FEATURES             Location/Qualifiers
     source          1..3070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..99)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(120..779)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     694..713
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     941..959
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(963..1006)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1009..1030
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1209..1917
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1926..1959
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      2052..2109
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2133..2150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2304..2892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2384..2403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
       61 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      121 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
      181 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      241 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      301 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
      361 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      421 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      481 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      541 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      601 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      661 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      721 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      781 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      841 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      961 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     1021 gcttctagag cgatgagtgg tctcgtcccg tcctaatacc tctacgtgca agcagtataa
     1081 aaacaaaata cataaacata actaaaatct catatcgttg taattgtcat atactagtgg
     1141 taattaagtc cagttacact tgtgccccaa ctggggtaac ctttgagttc tctcagttgg
     1201 gggcgtagct gctttaatga gatatgcgag aagcctatga tcgcatgata tttgctttca
     1261 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
     1321 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga aataatattc
     1381 tccgttcaat ttactgattg taccctacta cttatatgta caatattaaa atgaaaacaa
     1441 tatattgtgc tgaataggtt tatagcgaca tctatgatag agcgccacaa taacaaacaa
     1501 ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg cagaaccggt caaacctaaa
     1561 agactgatta cataaatctt attcaaattt caaaagtgcc ccaggggcta gtatctacga
     1621 cacaccgagc ggcgaactaa taacgctcac tgaagggaac tccggttccc cgccggcgcg
     1681 catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa gttacgggca
     1741 ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg agaattatgc
     1801 agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg
     1861 acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc tcagcagcgg
     1921 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacaag gttaaatttg
     1981 ctgactcttt tttacctttc atttctgaaa aatgtgagac cactcatcgc gactagtagc
     2041 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     2101 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     2161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2281 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     2341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3061 agctcgaggc
//
