LOCUS       sequence-447730-        3040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OCS terminator flanked by two flippase recognition target (FRT) 
            sites located at position 112 of the SlDFR minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Garcia-Perez E, Vazquez-Vilar M, Lozano-Duran R, Orzaez D
  TITLE     CuBe: a geminivirus-based copper-regulated expression system 
            suitable for post-harvest activation.
  JOURNAL   Plant Biotechnol J. 2025 Jan;23(1):141-155. doi: 10.1111/pbi.14485. 
            Epub 2024 Oct 22.
  PUBMED    39435699
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14485";
            journalName: "Plant Biotechnol J"; date: "2025-01"; volume: "23"; 
            issue: "1"; pages: "141-155"
COMMENT     Sequence Label: pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..99)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(120..779)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     694..713
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     941..959
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(963..1006)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1009..1030
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    1162..1195
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1196..1903
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1904..1937
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      2022..2079
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2103..2120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2274..2862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2354..2373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
       61 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      121 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
      181 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      241 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      301 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
      361 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      421 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      481 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      541 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      601 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      661 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      721 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      781 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      841 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      961 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     1021 gcttctagag cgatgagtgg tctcgtcccg tcctaatacc tctacgtgca agcagtataa
     1081 aaacaaaata cataaacata actaaaatct catatcgttg taattgtcat atactagtgg
     1141 taattaagtc cagttacact tgaagttcct attctctaga aagtatagga acttcctgct
     1201 ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt ctgttgtgca
     1261 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct
     1321 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac
     1381 tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata ttgtgctgaa
     1441 taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc gttttattat
     1501 tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac tgattacata
     1561 aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca ccgagcggcg
     1621 aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg ggtgagattc
     1681 cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat tcaacccggt
     1741 ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca tttttttggt
     1801 gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc
     1861 gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaagttc ctattctcta
     1921 gaaagtatag gaacttcaag gttaaatttg ctgactcttt tttacctttc atttctgaaa
     1981 aatgtgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag
     2041 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
     2101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     2281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3001 taaagtatat atgagtaaac ttggtctgac agctcgaggc
//
